Restriction map for
pFN21AE5717
(Linear) (Six-base) MAPSORT of: KIEE5717.seq Check: 5997 from: 1 to: 1971
KIEE5717.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1537 1971
Size: 1537 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1971
Size: 325 1646
AfeI AGC'GCT
Cuts at: 0 595 1971
Size: 595 1376
AflII C'TTAA_G
Cuts at: 0 1128 1971
Size: 1128 843
AflIII A'CryG_T
Cuts at: 0 212 1971
Size: 212 1759
AhdI GACnn_n'nnGTC
Cuts at: 0 129 1971
Size: 129 1842
AlwNI CAG_nnn'CTG
Cuts at: 0 194 399 606 1585 1645 1765 1825 1971
Size: 194 205 207 979 60 120 60 146
Fragments arranged by size:
979 207 205 194 146 120 60 60
ApaI G_GGCC'C
Cuts at: 0 863 1034 1529 1971
Size: 863 171 495 442
Fragments arranged by size:
863 495 442 171
ApaLI G'TGCA_C
Cuts at: 0 811 1971
Size: 811 1160
ApoI r'AATT_y
Cuts at: 0 1236 1971
Size: 1236 735
AvaI C'yCGr_G
Cuts at: 0 673 910 946 1971
Size: 673 237 36 1025
Fragments arranged by size:
1025 673 237 36
BamHI G'GATC_C
Cuts at: 0 536 1971
Size: 536 1435
BanI G'GyrC_C
Cuts at: 0 687 1423 1466 1537 1971
Size: 687 736 43 71 434
Fragments arranged by size:
736 687 434 71 43
BanII G_rGCy'C
Cuts at: 0 518 863 919 1034 1055 1127 1529 1552
Size: 518 345 56 115 21 72 402 23
Cuts at: 1552 1612 1672 1732 1792 1852 1971
Size: 60 60 60 60 60 119
Fragments arranged by size:
518 402 345 119 115 72 60 60
60 60 60 56 23 21
BbeI G_GCGC'C
Cuts at: 0 691 1971
Size: 691 1280
BbsI GAAGACnn'nnnn_
Cuts at: 0 1940 1971
Size: 1940 31
BbvCI CC'TCA_GC
Cuts at: 0 1255 1971
Size: 1255 716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 62 1971
Size: 28 34 1909
Fragments arranged by size:
1909 34 28
BglI GCCn_nnn'nGGC
Cuts at: 0 1351 1971
Size: 1351 620
BlpI GC'TnA_GC
Cuts at: 0 1165 1971
Size: 1165 806
Bme1580I G_kGCm'C
Cuts at: 0 565 759 815 863 1034 1254 1428 1469
Size: 565 194 56 48 171 220 174 41
Cuts at: 1469 1529 1971
Size: 60 442
Fragments arranged by size:
565 442 220 194 174 171 60 56
48 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 730 903 1093 1180 1971
Size: 565 165 173 190 87 791
Fragments arranged by size:
791 565 190 173 165 87
Bpu10I CC'TnA_GC
Cuts at: 0 451 1255 1971
Size: 451 804 716
Fragments arranged by size:
804 716 451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 634 1971
Size: 391 243 1337
Fragments arranged by size:
1337 391 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 1552 1612 1672 1732 1792 1852 1971
Size: 1552 60 60 60 60 60 119
Fragments arranged by size:
1552 119 60 60 60 60 60
BsaHI Gr'CG_yC
Cuts at: 0 688 1971
Size: 688 1283
BsaWI w'CCGG_w
Cuts at: 0 237 1971
Size: 237 1734
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 367 397 1334 1364 1971
Size: 4 30 333 30 937 30 607
Fragments arranged by size:
937 607 333 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 55 142 151 727 946 1092 1516
Size: 46 9 87 9 576 219 146 424
Cuts at: 1516 1971
Size: 455
Fragments arranged by size:
576 455 424 219 146 87 46 9
9
BseYI C'CCAG_C
Cuts at: 0 157 522 657 666 889 1034 1393 1570
Size: 157 365 135 9 223 145 359 177
Cuts at: 1570 1575 1635 1690 1755 1870 1971
Size: 5 60 55 65 115 101
Fragments arranged by size:
365 359 223 177 157 145 135 115
101 65 60 55 9 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1971
Size: 1339 632
BsiHKAI G_wGCw'C
Cuts at: 0 518 579 680 815 830 1971
Size: 518 61 101 135 15 1141
Fragments arranged by size:
1141 518 135 101 61 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 709 1971
Size: 709 1262
Bsp1286I G_dGCh'C
Cuts at: 0 518 565 579 680 759 815 830 863
Size: 518 47 14 101 79 56 15 33
Cuts at: 863 919 1034 1055 1127 1254 1428 1469 1529
Size: 56 115 21 72 127 174 41 60
Cuts at: 1529 1552 1612 1672 1732 1792 1852 1971
Size: 23 60 60 60 60 60 119
Fragments arranged by size:
518 174 127 119 115 101 79 72
60 60 60 60 60 60 56 56
47 41 33 23 21 15 14
BspEI T'CCGG_A
Cuts at: 0 237 1971
Size: 237 1734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1593 1653 1773 1833 1971
Size: 1593 60 120 60 138
Fragments arranged by size:
1593 138 120 60 60
BsrBI CCG'CTC
Cuts at: 0 495 909 1971
Size: 495 414 1062
Fragments arranged by size:
1062 495 414
BsrDI GCAATG_nn'
Cuts at: 0 267 1971
Size: 267 1704
BsrFI r'CCGG_y
Cuts at: 0 91 1169 1971
Size: 91 1078 802
Fragments arranged by size:
1078 802 91
BssSI C'ACGA_G
Cuts at: 0 204 961 1971
Size: 204 757 1010
Fragments arranged by size:
1010 757 204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1585 1645 1765 1825 1971
Size: 1585 60 120 60 146
Fragments arranged by size:
1585 146 120 60 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 927 1971
Size: 927 1044
BstYI r'GATC_y
Cuts at: 0 240 299 485 536 1971
Size: 240 59 186 51 1435
Fragments arranged by size:
1435 240 186 59 51
Bsu36I CC'TnA_GG
Cuts at: 0 1270 1971
Size: 1270 701
BtgI C'CryG_G
Cuts at: 0 142 1039 1104 1971
Size: 142 897 65 867
Fragments arranged by size:
897 867 142 65
BtsI GCAGTG_nn'
Cuts at: 0 1322 1971
Size: 1322 649
DraI TTT'AAA
Cuts at: 0 471 1971
Size: 471 1500
EaeI y'GGCC_r
Cuts at: 0 1352 1412 1971
Size: 1352 60 559
Fragments arranged by size:
1352 559 60
EarI CTCTTCn'nnn_
Cuts at: 0 1144 1971
Size: 1144 827
EcoICRI GAG'CTC
Cuts at: 0 516 1971
Size: 516 1455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 565 730 903 1093 1180 1971
Size: 325 240 165 173 190 87 791
Fragments arranged by size:
791 325 240 190 173 165 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 1258 1971
Size: 370 888 713
Fragments arranged by size:
888 713 370
EcoO109I rG'GnC_Cy
Cuts at: 0 859 860 951 1030 1031 1066 1198 1280
Size: 859 1 91 79 1 35 132 82
Cuts at: 1280 1302 1448 1525 1567 1582 1627 1642 1687
Size: 22 146 77 42 15 45 15 45
Cuts at: 1687 1702 1747 1762 1807 1822 1867 1882 1971
Size: 15 45 15 45 15 45 15 89
Fragments arranged by size:
859 146 132 91 89 82 79 77
45 45 45 45 45 42 35 22
15 15 15 15 15 15 1 1
HaeII r_GCGC'y
Cuts at: 0 597 691 801 1971
Size: 597 94 110 1170
Fragments arranged by size:
1170 597 110 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 115 147 367 399 1597 1629
Size: 4 32 79 32 220 32 1198 32
Cuts at: 1629 1657 1689 1717 1749 1777 1809 1837 1869
Size: 28 32 28 32 28 32 28 32
Cuts at: 1869 1930 1962 1971
Size: 61 32 9
Fragments arranged by size:
1198 220 79 61 32 32 32 32
32 32 32 32 32 28 28 28
28 9 4
KasI G'GCGC_C
Cuts at: 0 687 1971
Size: 687 1284
KpnI G_GTAC'C
Cuts at: 0 1541 1971
Size: 1541 430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 961 1971
Size: 113 848 1010
Fragments arranged by size:
1010 848 113
MscI TGG'CCA
Cuts at: 0 1414 1971
Size: 1414 557
MspA1I CmG'CkG
Cuts at: 0 144 693 807 842 1971
Size: 144 549 114 35 1129
Fragments arranged by size:
1129 549 144 114 35
NaeI GCC'GGC
Cuts at: 0 93 1171 1971
Size: 93 1078 800
Fragments arranged by size:
1078 800 93
NarI GG'CG_CC
Cuts at: 0 688 1971
Size: 688 1283
NgoMIV G'CCGG_C
Cuts at: 0 91 1169 1971
Size: 91 1078 802
Fragments arranged by size:
1078 802 91
NspI r_CATG'y
Cuts at: 0 216 436 1971
Size: 216 220 1535
Fragments arranged by size:
1535 220 216
PasI CC'CwG_GG
Cuts at: 0 975 1306 1338 1543 1971
Size: 975 331 32 205 428
Fragments arranged by size:
975 428 331 205 32
PciI A'CATG_T
Cuts at: 0 212 1971
Size: 212 1759
PflMI CCAn_nnn'nTGG
Cuts at: 0 194 1290 1338 1434 1971
Size: 194 1096 48 96 537
Fragments arranged by size:
1096 537 194 96 48
PfoI T'CCnGG_A
Cuts at: 0 488 707 919 1971
Size: 488 219 212 1052
Fragments arranged by size:
1052 488 219 212
PpuMI rG'GwC_Cy
Cuts at: 0 951 1066 1280 1302 1448 1582 1642 1702
Size: 951 115 214 22 146 134 60 60
Cuts at: 1702 1762 1822 1882 1971
Size: 60 60 60 89
Fragments arranged by size:
951 214 146 134 115 89 60 60
60 60 60 22
PspOMI G'GGCC_C
Cuts at: 0 859 1030 1525 1971
Size: 859 171 495 446
Fragments arranged by size:
859 495 446 171
PspXI vC'TCGA_Gb
Cuts at: 0 673 910 1971
Size: 673 237 1061
Fragments arranged by size:
1061 673 237
PstI C_TGCA'G
Cuts at: 0 1590 1650 1770 1830 1971
Size: 1590 60 120 60 141
Fragments arranged by size:
1590 141 120 60 60
PvuII CAG'CTG
Cuts at: 0 807 1971
Size: 807 1164
SacI G_AGCT'C
Cuts at: 0 518 1971
Size: 518 1453
SacII CC_GC'GG
Cuts at: 0 145 1971
Size: 145 1826
SanDI GG'GwC_CC
Cuts at: 0 951 1066 1280 1448 1971
Size: 951 115 214 168 523
Fragments arranged by size:
951 523 214 168 115
SbfI CC_TGCA'GG
Cuts at: 0 1590 1650 1770 1830 1971
Size: 1590 60 120 60 141
Fragments arranged by size:
1590 141 120 60 60
SfcI C'TryA_G
Cuts at: 0 288 1586 1646 1766 1826 1971
Size: 288 1298 60 120 60 145
Fragments arranged by size:
1298 288 145 120 60 60
SfoI GGC'GCC
Cuts at: 0 689 1971
Size: 689 1282
SgrAI Cr'CCGG_yG
Cuts at: 0 91 1971
Size: 91 1880
SmaI CCC'GGG
Cuts at: 0 948 1971
Size: 948 1023
SmlI C'TyrA_G
Cuts at: 0 370 649 673 910 1128 1971
Size: 370 279 24 237 218 843
Fragments arranged by size:
843 370 279 237 218 24
SphI G_CATG'C
Cuts at: 0 436 1971
Size: 436 1535
StyI C'CwwG_G
Cuts at: 0 1433 1472 1904 1971
Size: 1433 39 432 67
Fragments arranged by size:
1433 432 67 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 649 988 1971
Size: 566 83 339 983
Fragments arranged by size:
983 566 339 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1334 1366 1971
Size: 1334 32 605
Fragments arranged by size:
1334 605 32
Tth111I GACn'n_nGTC
Cuts at: 0 183 1971
Size: 183 1788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 425 1971
Size: 425 1546
XhoI C'TCGA_G
Cuts at: 0 673 910 1971
Size: 673 237 1061
Fragments arranged by size:
1061 673 237
XmaI C'CCGG_G
Cuts at: 0 946 1971
Size: 946 1025
XmnI GAAnn'nnTTC
Cuts at: 0 595 1971
Size: 595 1376
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AhdI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
KpnI MmeI MscI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SbfI SfcI SfoI SgrAI
SmaI SmlI SphI StyI TaqII TstI Tth111I XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AleI AlfI AloI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BsaAI BsaBI BsiEI
BsiWI BsmI BspHI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SapI ScaI SexAI SfiI SgfI
SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI
ZraI