Restriction map for
pFN21AE5715
(Linear) (Six-base) MAPSORT of: KIEE5715.seq Check: 4497 from: 1 to: 1893
KIEE5715.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1893
Size: 1181 712
AflII C'TTAA_G
Cuts at: 0 619 651 826 1893
Size: 619 32 175 1067
Fragments arranged by size:
1067 619 175 32
AflIII A'CryG_T
Cuts at: 0 161 1893
Size: 161 1732
AgeI A'CCGG_T
Cuts at: 0 1876 1893
Size: 1876 17
AhdI GACnn_n'nnGTC
Cuts at: 0 1451 1893
Size: 1451 442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 743 1442 1604 1893
Size: 743 699 162 289
Fragments arranged by size:
743 699 289 162
AlwNI CAG_nnn'CTG
Cuts at: 0 358 1471 1893
Size: 358 1113 422
Fragments arranged by size:
1113 422 358
ApaI G_GGCC'C
Cuts at: 0 107 647 1893
Size: 107 540 1246
Fragments arranged by size:
1246 540 107
ApoI r'AATT_y
Cuts at: 0 216 588 779 1893
Size: 216 372 191 1114
Fragments arranged by size:
1114 372 216 191
AseI AT'TA_AT
Cuts at: 0 329 1893
Size: 329 1564
AvaI C'yCGr_G
Cuts at: 0 1774 1893
Size: 1774 119
BanI G'GyrC_C
Cuts at: 0 1303 1893
Size: 1303 590
BanII G_rGCy'C
Cuts at: 0 107 647 767 798 1893
Size: 107 540 120 31 1095
Fragments arranged by size:
1095 540 120 107 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1893
Size: 605 1288
BbvCI CC'TCA_GC
Cuts at: 0 1544 1893
Size: 1544 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 285 319 1893
Size: 285 34 1574
Fragments arranged by size:
1574 285 34
BclI T'GATC_A
Cuts at: 0 137 1893
Size: 137 1756
BlpI GC'TnA_GC
Cuts at: 0 1103 1893
Size: 1103 790
Bme1580I G_kGCm'C
Cuts at: 0 107 647 1178 1893
Size: 107 540 531 715
Fragments arranged by size:
715 540 531 107
BmgBI CAC'GTC
Cuts at: 0 63 1459 1893
Size: 63 1396 434
Fragments arranged by size:
1396 434 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 1328 1893
Size: 1328 565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 1766 1893
Size: 1611 155 127
Fragments arranged by size:
1611 155 127
Bpu10I CC'TnA_GC
Cuts at: 0 1298 1544 1893
Size: 1298 246 349
Fragments arranged by size:
1298 349 246
BsaI GGTCTCn'nnnn_
Cuts at: 0 263 1374 1893
Size: 263 1111 519
Fragments arranged by size:
1111 519 263
BsaAI yAC'GTr
Cuts at: 0 42 1893
Size: 42 1851
BsaWI w'CCGG_w
Cuts at: 0 119 929 1876 1893
Size: 119 810 947 17
Fragments arranged by size:
947 810 119 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 1011 1637 1893
Size: 141 870 626 256
Fragments arranged by size:
870 626 256 141
BseYI C'CCAG_C
Cuts at: 0 7 16 1717 1893
Size: 7 9 1701 176
Fragments arranged by size:
1701 176 9 7
BsiHKAI G_wGCw'C
Cuts at: 0 767 798 1191 1356 1893
Size: 767 31 393 165 537
Fragments arranged by size:
767 537 393 165 31
BsmI GAATG_Cn'
Cuts at: 0 485 1893
Size: 485 1408
BsmBI CGTCTCn'nnnn_
Cuts at: 0 931 1893
Size: 931 962
Bsp1286I G_dGCh'C
Cuts at: 0 107 647 767 798 1178 1191 1356 1893
Size: 107 540 120 31 380 13 165 537
Fragments arranged by size:
540 537 380 165 120 107 31 13
BspEI T'CCGG_A
Cuts at: 0 119 929 1893
Size: 119 810 964
Fragments arranged by size:
964 810 119
BspHI T'CATG_A
Cuts at: 0 1208 1893
Size: 1208 685
BsrFI r'CCGG_y
Cuts at: 0 1876 1893
Size: 1876 17
BsrGI T'GTAC_A
Cuts at: 0 426 1599 1893
Size: 426 1173 294
Fragments arranged by size:
1173 426 294
BssSI C'ACGA_G
Cuts at: 0 1403 1893
Size: 1403 490
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1471 1893
Size: 1471 422
BstBI TT'CG_AA
Cuts at: 0 279 1893
Size: 279 1614
BstYI r'GATC_y
Cuts at: 0 122 624 1893
Size: 122 502 1269
Fragments arranged by size:
1269 502 122
BtgI C'CryG_G
Cuts at: 0 1510 1893
Size: 1510 383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 88 1893
Size: 88 1805
EaeI y'GGCC_r
Cuts at: 0 1073 1893
Size: 1073 820
EarI CTCTTCn'nnn_
Cuts at: 0 1109 1893
Size: 1109 784
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1333 1893
Size: 1333 560
EcoICRI GAG'CTC
Cuts at: 0 765 796 1893
Size: 765 31 1097
Fragments arranged by size:
1097 765 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1611 1766 1893
Size: 1181 430 155 127
Fragments arranged by size:
1181 430 155 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1196 1893
Size: 1196 697
EcoO109I rG'GnC_Cy
Cuts at: 0 48 103 1893
Size: 48 55 1790
Fragments arranged by size:
1790 55 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 562 645 1893
Size: 562 83 1248
Fragments arranged by size:
1248 562 83
FspI TGC'GCA
Cuts at: 0 1501 1893
Size: 1501 392
FspAI rTGC'GCAy
Cuts at: 0 1501 1893
Size: 1501 392
HaeII r_GCGC'y
Cuts at: 0 618 1122 1893
Size: 618 504 771
Fragments arranged by size:
771 618 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1437 1469 1893
Size: 1437 32 424
Fragments arranged by size:
1437 424 32
HincII GTy'rAC
Cuts at: 0 1415 1866 1893
Size: 1415 451 27
Fragments arranged by size:
1415 451 27
HindIII A'AGCT_T
Cuts at: 0 708 1893
Size: 708 1185
HpaI GTT'AAC
Cuts at: 0 1866 1893
Size: 1866 27
MluI A'CGCG_T
Cuts at: 0 161 1893
Size: 161 1732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 489 1721 1893
Size: 179 310 1232 172
Fragments arranged by size:
1232 310 179 172
MscI TGG'CCA
Cuts at: 0 1075 1893
Size: 1075 818
MslI CAynn'nnrTG
Cuts at: 0 725 1556 1732 1893
Size: 725 831 176 161
Fragments arranged by size:
831 725 176 161
MspA1I CmG'CkG
Cuts at: 0 146 451 1216 1468 1893
Size: 146 305 765 252 425
Fragments arranged by size:
765 425 305 252 146
NspI r_CATG'y
Cuts at: 0 202 1737 1893
Size: 202 1535 156
Fragments arranged by size:
1535 202 156
PpuMI rG'GwC_Cy
Cuts at: 0 48 1893
Size: 48 1845
PshAI GACnn'nnGTC
Cuts at: 0 1216 1893
Size: 1216 677
PsiI TTA'TAA
Cuts at: 0 229 1893
Size: 229 1664
PspOMI G'GGCC_C
Cuts at: 0 103 643 1893
Size: 103 540 1250
Fragments arranged by size:
1250 540 103
PspXI vC'TCGA_Gb
Cuts at: 0 1774 1893
Size: 1774 119
PstI C_TGCA'G
Cuts at: 0 130 1467 1485 1893
Size: 130 1337 18 408
Fragments arranged by size:
1337 408 130 18
PvuII CAG'CTG
Cuts at: 0 451 1216 1468 1893
Size: 451 765 252 425
Fragments arranged by size:
765 451 425 252
SacI G_AGCT'C
Cuts at: 0 767 798 1893
Size: 767 31 1095
Fragments arranged by size:
1095 767 31
SapI GCTCTTCn'nnn_
Cuts at: 0 1109 1893
Size: 1109 784
ScaI AGT'ACT
Cuts at: 0 1374 1893
Size: 1374 519
SexAI A'CCwGG_T
Cuts at: 0 872 1893
Size: 872 1021
SfcI C'TryA_G
Cuts at: 0 126 740 1463 1481 1814 1893
Size: 126 614 723 18 333 79
Fragments arranged by size:
723 614 333 126 79 18
SmlI C'TyrA_G
Cuts at: 0 619 651 826 1774 1893
Size: 619 32 175 948 119
Fragments arranged by size:
948 619 175 119 32
SpeI A'CTAG_T
Cuts at: 0 961 1893
Size: 961 932
SphI G_CATG'C
Cuts at: 0 202 1893
Size: 202 1691
StyI C'CwwG_G
Cuts at: 0 410 1893
Size: 410 1483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 685 1893
Size: 685 1208
TatI w'GTAC_w
Cuts at: 0 426 1372 1599 1821 1893
Size: 426 946 227 222 72
Fragments arranged by size:
946 426 227 222 72
Tth111I GACn'n_nGTC
Cuts at: 0 1280 1893
Size: 1280 613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1286 1893
Size: 1286 607
XhoI C'TCGA_G
Cuts at: 0 1774 1893
Size: 1774 119
Enzymes that do cut:
AcuI AflII AflIII AgeI AhdI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BbvCI BcgI
BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI MluI MmeI MscI MslI MspA1I NspI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SpeI SphI StyI TaqII
TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BciVI
BfrBI BglI BglII BmtI BplI BpuEI BsaBI BsaHI
BsaXI BsgI BsiEI BsiWI BspMI BsrBI BsrDI BssHII
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI KasI KpnI
MfeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SspI StuI SwaI TstI XbaI XmaI XmnI ZraI