Restriction map for
pFN21AE5712
(Linear) (Six-base) MAPSORT of: KIEE5712.seq Check: 605 from: 1 to: 2196
KIEE5712.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AatII G_ACGT'C
Cuts at: 0 551 2196
Size: 551 1645
AccI GT'mk_AC
Cuts at: 0 944 1009 2196
Size: 944 65 1187
Fragments arranged by size:
1187 944 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 601 2196
Size: 278 323 1595
Fragments arranged by size:
1595 323 278
AfeI AGC'GCT
Cuts at: 0 410 2196
Size: 410 1786
AleI CACnn'nnGTG
Cuts at: 0 1180 2196
Size: 1180 1016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 1105 1414 2196
Size: 682 423 309 782
Fragments arranged by size:
782 682 423 309
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1694 1726 2196
Size: 1694 32 470
Fragments arranged by size:
1694 470 32
AlwNI CAG_nnn'CTG
Cuts at: 0 15 241 360 1161 1817 1919 2196
Size: 15 226 119 801 656 102 277
Fragments arranged by size:
801 656 277 226 119 102 15
ApaI G_GGCC'C
Cuts at: 0 97 340 756 1529 2196
Size: 97 243 416 773 667
Fragments arranged by size:
773 667 416 243 97
ApaLI G'TGCA_C
Cuts at: 0 136 2196
Size: 136 2060
AvaI C'yCGr_G
Cuts at: 0 28 263 2196
Size: 28 235 1933
Fragments arranged by size:
1933 235 28
BanI G'GyrC_C
Cuts at: 0 298 2196
Size: 298 1898
BanII G_rGCy'C
Cuts at: 0 29 97 340 756 800 881 925 1370
Size: 29 68 243 416 44 81 44 445
Cuts at: 1370 1529 1864 2196
Size: 159 335 332
Fragments arranged by size:
445 416 335 332 243 159 81 68
44 44 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 1996 2196
Size: 635 1361 200
Fragments arranged by size:
1361 635 200
BbvCI CC'TCA_GC
Cuts at: 0 1933 2196
Size: 1933 263
BclI T'GATC_A
Cuts at: 0 1838 2196
Size: 1838 358
BglI GCCn_nnn'nGGC
Cuts at: 0 1822 2091 2196
Size: 1822 269 105
Fragments arranged by size:
1822 269 105
BlpI GC'TnA_GC
Cuts at: 0 19 76 277 919 1142 2196
Size: 19 57 201 642 223 1054
Fragments arranged by size:
1054 642 223 201 57 19
Bme1580I G_kGCm'C
Cuts at: 0 97 140 340 756 1189 1502 1529 2036
Size: 97 43 200 416 433 313 27 507
Cuts at: 2036 2067 2196
Size: 31 129
Fragments arranged by size:
507 433 416 313 200 129 97 43
31 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 642 1395 2196
Size: 642 753 801
Fragments arranged by size:
801 753 642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 900 1083 2196
Size: 900 183 1113
Fragments arranged by size:
1113 900 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 382 433 779 1229 1375 1466 1696
Size: 347 35 51 346 450 146 91 230
Cuts at: 1696 2196
Size: 500
Fragments arranged by size:
500 450 347 346 230 146 91 51
35
Bpu10I CC'TnA_GC
Cuts at: 0 58 1933 2196
Size: 58 1875 263
Fragments arranged by size:
1875 263 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 730 2053 2196
Size: 373 357 1323 143
Fragments arranged by size:
1323 373 357 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1798 2196
Size: 253 1545 398
Fragments arranged by size:
1545 398 253
BsaAI yAC'GTr
Cuts at: 0 852 2196
Size: 852 1344
BsaBI GATnn'nnATC
Cuts at: 0 1843 2196
Size: 1843 353
BsaHI Gr'CG_yC
Cuts at: 0 548 2196
Size: 548 1648
BsaWI w'CCGG_w
Cuts at: 0 128 380 803 2196
Size: 128 252 423 1393
Fragments arranged by size:
1393 423 252 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1694 1724 1834 1864 2128 2158 2196
Size: 1694 30 110 30 264 30 38
Fragments arranged by size:
1694 264 110 38 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 871 1372 2196
Size: 46 825 501 824
Fragments arranged by size:
825 824 501 46
BseYI C'CCAG_C
Cuts at: 0 1053 1279 1563 2196
Size: 1053 226 284 633
Fragments arranged by size:
1053 633 284 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 2196
Size: 1450 746
BsiHKAI G_wGCw'C
Cuts at: 0 140 671 881 1712 2171 2196
Size: 140 531 210 831 459 25
Fragments arranged by size:
831 531 459 210 140 25
BsmI GAATG_Cn'
Cuts at: 0 2166 2196
Size: 2166 30
Bsp1286I G_dGCh'C
Cuts at: 0 29 97 140 340 671 756 800 881
Size: 29 68 43 200 331 85 44 81
Cuts at: 881 925 1189 1370 1502 1529 1712 1864 2036
Size: 44 264 181 132 27 183 152 172
Cuts at: 2036 2067 2171 2196
Size: 31 104 25
Fragments arranged by size:
331 264 200 183 181 172 152 132
104 85 81 68 44 44 43 31
29 27 25
BspHI T'CATG_A
Cuts at: 0 2156 2196
Size: 2156 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 824 956 2196
Size: 249 575 132 1240
Fragments arranged by size:
1240 575 249 132
BsrDI GCAATG_nn'
Cuts at: 0 1665 2196
Size: 1665 531
BsrFI r'CCGG_y
Cuts at: 0 1151 2024 2196
Size: 1151 873 172
Fragments arranged by size:
1151 873 172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 1919 2196
Size: 241 1678 277
Fragments arranged by size:
1678 277 241
BstEII G'GTnAC_C
Cuts at: 0 102 1017 2196
Size: 102 915 1179
Fragments arranged by size:
1179 915 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 1789 2196
Size: 543 1246 407
Fragments arranged by size:
1246 543 407
BstYI r'GATC_y
Cuts at: 0 1073 2196
Size: 1073 1123
BstZ17I GTA'TAC
Cuts at: 0 1010 2196
Size: 1010 1186
Bsu36I CC'TnA_GG
Cuts at: 0 1982 2196
Size: 1982 214
BtgI C'CryG_G
Cuts at: 0 1502 1518 1619 1761 2196
Size: 1502 16 101 142 435
Fragments arranged by size:
1502 435 142 101 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 575 1830 2196
Size: 575 1255 366
Fragments arranged by size:
1255 575 366
BtsI GCAGTG_nn'
Cuts at: 0 1544 1703 1920 2196
Size: 1544 159 217 276
Fragments arranged by size:
1544 276 217 159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1608 1643 1721 1756 2196
Size: 1608 35 78 35 440
Fragments arranged by size:
1608 440 78 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 146 2196
Size: 146 2050
EaeI y'GGCC_r
Cuts at: 0 120 1629 2196
Size: 120 1509 567
Fragments arranged by size:
1509 567 120
EarI CTCTTCn'nnn_
Cuts at: 0 341 912 2196
Size: 341 571 1284
Fragments arranged by size:
1284 571 341
EcoICRI GAG'CTC
Cuts at: 0 879 2196
Size: 879 1317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 347 382 433 601 779 1229 1375
Size: 278 69 35 51 168 178 450 146
Cuts at: 1375 1466 1696 2196
Size: 91 230 500
Fragments arranged by size:
500 450 278 230 178 168 146 91
69 51 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1319 1357 2196
Size: 1319 38 839
Fragments arranged by size:
1319 839 38
EcoO109I rG'GnC_Cy
Cuts at: 0 54 159 238 337 717 753 1158 1251
Size: 54 105 79 99 380 36 405 93
Cuts at: 1251 1525 1526 1814 2196
Size: 274 1 288 382
Fragments arranged by size:
405 382 380 288 274 105 99 93
79 54 36 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1602 2196
Size: 1602 594
FspI TGC'GCA
Cuts at: 0 319 2196
Size: 319 1877
HaeII r_GCGC'y
Cuts at: 0 83 412 510 2196
Size: 83 329 98 1686
Fragments arranged by size:
1686 329 98 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 715 747 957 989 2128 2160
Size: 205 32 478 32 210 32 1139 32
Cuts at: 2160 2196
Size: 36
Fragments arranged by size:
1139 478 210 205 36 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 1241 2196
Size: 1241 955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 2196
Size: 1315 881
MscI TGG'CCA
Cuts at: 0 1631 2196
Size: 1631 565
MslI CAynn'nnrTG
Cuts at: 0 664 703 1180 1396 1673 1727 2196
Size: 664 39 477 216 277 54 469
Fragments arranged by size:
664 477 469 277 216 54 39
MspA1I CmG'CkG
Cuts at: 0 990 1053 1111 1207 2196
Size: 990 63 58 96 989
Fragments arranged by size:
990 989 96 63 58
NaeI GCC'GGC
Cuts at: 0 1153 2196
Size: 1153 1043
NcoI C'CATG_G
Cuts at: 0 1502 1761 2196
Size: 1502 259 435
Fragments arranged by size:
1502 435 259
NgoMIV G'CCGG_C
Cuts at: 0 1151 2196
Size: 1151 1045
PasI CC'CwG_GG
Cuts at: 0 340 2196
Size: 340 1856
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 1125 2196
Size: 201 924 1071
Fragments arranged by size:
1071 924 201
PfoI T'CCnGG_A
Cuts at: 0 359 1712 2196
Size: 359 1353 484
Fragments arranged by size:
1353 484 359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1694 1726 2196
Size: 340 32 1322 32 470
Fragments arranged by size:
1322 470 340 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 159 238 717 1158 1251 2196
Size: 159 79 479 441 93 945
Fragments arranged by size:
945 479 441 159 93 79
PshAI GACnn'nnGTC
Cuts at: 0 932 2196
Size: 932 1264
PspOMI G'GGCC_C
Cuts at: 0 93 336 752 1525 2196
Size: 93 243 416 773 671
Fragments arranged by size:
773 671 416 243 93
PstI C_TGCA'G
Cuts at: 0 246 953 1084 1323 1469 2196
Size: 246 707 131 239 146 727
Fragments arranged by size:
727 707 246 239 146 131
PvuII CAG'CTG
Cuts at: 0 990 1053 1111 1207 2196
Size: 990 63 58 96 989
Fragments arranged by size:
990 989 96 63 58
SacI G_AGCT'C
Cuts at: 0 881 2196
Size: 881 1315
SanDI GG'GwC_CC
Cuts at: 0 717 2196
Size: 717 1479
SbfI CC_TGCA'GG
Cuts at: 0 246 2196
Size: 246 1950
SexAI A'CCwGG_T
Cuts at: 0 200 2196
Size: 200 1996
SfcI C'TryA_G
Cuts at: 0 242 949 1080 1319 1402 1465 1809 2196
Size: 242 707 131 239 83 63 344 387
Fragments arranged by size:
707 387 344 242 239 131 83 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1822 2196
Size: 1822 374
SmaI CCC'GGG
Cuts at: 0 265 2196
Size: 265 1931
SmlI C'TyrA_G
Cuts at: 0 388 709 2068 2196
Size: 388 321 1359 128
Fragments arranged by size:
1359 388 321 128
SpeI A'CTAG_T
Cuts at: 0 1599 2196
Size: 1599 597
StyI C'CwwG_G
Cuts at: 0 512 542 1013 1502 1761 2196
Size: 512 30 471 489 259 435
Fragments arranged by size:
512 489 471 435 259 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1192 1293 1335 1741 2131 2196
Size: 1192 101 42 406 390 65
Fragments arranged by size:
1192 406 390 101 65 42
XmaI C'CCGG_G
Cuts at: 0 263 2196
Size: 263 1933
XmnI GAAnn'nnTTC
Cuts at: 0 355 2196
Size: 355 1841
ZraI GAC'GTC
Cuts at: 0 549 2196
Size: 549 1647
Enzymes that do cut:
AatII AccI AcuI AfeI AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BbvCI BclI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SbfI SexAI SfcI SfiI SmaI SmlI SpeI StyI
TaqII XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BglII BmgBI BmtI BsiEI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI ClaI DraI
DrdI EagI EciI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
ScaI SfoI SgfI SgrAI SnaBI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI