Restriction map for
pFN21AE5711
(Linear) (Six-base) MAPSORT of: KIEE5711.seq Check: 1193 from: 1 to: 2415
KIEE5711.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 345 677 920 2415
Size: 345 332 243 1495
Fragments arranged by size:
1495 345 332 243
AclI AA'CG_TT
Cuts at: 0 2401 2415
Size: 2401 14
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 847 2415
Size: 179 668 1568
Fragments arranged by size:
1568 668 179
AflII C'TTAA_G
Cuts at: 0 1872 2415
Size: 1872 543
AflIII A'CryG_T
Cuts at: 0 1268 1661 2415
Size: 1268 393 754
Fragments arranged by size:
1268 754 393
AleI CACnn'nnGTG
Cuts at: 0 391 895 2415
Size: 391 504 1520
Fragments arranged by size:
1520 504 391
AlwNI CAG_nnn'CTG
Cuts at: 0 33 206 430 537 1040 1154 1992 2083
Size: 33 173 224 107 503 114 838 91
Cuts at: 2083 2415
Size: 332
Fragments arranged by size:
838 503 332 224 173 114 107 91
33
ApaI G_GGCC'C
Cuts at: 0 490 716 861 1612 2415
Size: 490 226 145 751 803
Fragments arranged by size:
803 751 490 226 145
ApoI r'AATT_y
Cuts at: 0 2116 2415
Size: 2116 299
AvaI C'yCGr_G
Cuts at: 0 893 1225 1561 2415
Size: 893 332 336 854
Fragments arranged by size:
893 854 336 332
BamHI G'GATC_C
Cuts at: 0 826 2415
Size: 826 1589
BanI G'GyrC_C
Cuts at: 0 985 2415
Size: 985 1430
BanII G_rGCy'C
Cuts at: 0 407 490 716 861 1121 1612 1913 2415
Size: 407 83 226 145 260 491 301 502
Fragments arranged by size:
502 491 407 301 260 226 145 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 647 1074 1626 1635 2415
Size: 647 427 552 9 780
Fragments arranged by size:
780 647 552 427 9
BbvCI CC'TCA_GC
Cuts at: 0 1144 2415
Size: 1144 1271
BglI GCCn_nnn'nGGC
Cuts at: 0 2063 2415
Size: 2063 352
Bme1580I G_kGCm'C
Cuts at: 0 490 716 861 892 1612 2415
Size: 490 226 145 31 720 803
Fragments arranged by size:
803 720 490 226 145 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 783 1050 1891 2415
Size: 783 267 841 524
Fragments arranged by size:
841 783 524 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 121 354 452 668 1135 2014 2170
Size: 105 16 233 98 216 467 879 156
Cuts at: 2170 2233 2415
Size: 63 182
Fragments arranged by size:
879 467 233 216 182 156 105 98
63 16
Bpu10I CC'TnA_GC
Cuts at: 0 1144 2415
Size: 1144 1271
BsaAI yAC'GTr
Cuts at: 0 1271 2415
Size: 1271 1144
BsaBI GATnn'nnATC
Cuts at: 0 1946 2415
Size: 1946 469
BsaWI w'CCGG_w
Cuts at: 0 1550 2415
Size: 1550 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 1834 1864 2087 2117 2415
Size: 859 30 945 30 223 30 298
Fragments arranged by size:
945 859 298 223 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1389 1720 2383 2388 2415
Size: 1389 331 663 5 27
Fragments arranged by size:
1389 663 331 27 5
BseYI C'CCAG_C
Cuts at: 0 42 582 1391 2099 2286 2415
Size: 42 540 809 708 187 129
Fragments arranged by size:
809 708 540 187 129 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 863 1834 2415
Size: 96 767 971 581
Fragments arranged by size:
971 767 581 96
BsiHKAI G_wGCw'C
Cuts at: 0 407 1121 1655 1913 2415
Size: 407 714 534 258 502
Fragments arranged by size:
714 534 502 407 258
BsmI GAATG_Cn'
Cuts at: 0 9 1329 2415
Size: 9 1320 1086
Fragments arranged by size:
1320 1086 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 2415
Size: 169 2246
Bsp1286I G_dGCh'C
Cuts at: 0 407 490 716 861 892 1121 1612 1655
Size: 407 83 226 145 31 229 491 43
Cuts at: 1655 1913 2415
Size: 258 502
Fragments arranged by size:
502 491 407 258 229 226 145 83
43 31
BspEI T'CCGG_A
Cuts at: 0 1550 2415
Size: 1550 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1496 2415
Size: 1496 919
BsrDI GCAATG_nn'
Cuts at: 0 624 2415
Size: 624 1791
BsrFI r'CCGG_y
Cuts at: 0 1130 2415
Size: 1130 1285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 33 2415
Size: 33 2382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2306 2415
Size: 2306 109
BstYI r'GATC_y
Cuts at: 0 826 1109 1844 2415
Size: 826 283 735 571
Fragments arranged by size:
826 735 571 283
Bsu36I CC'TnA_GG
Cuts at: 0 20 301 952 2415
Size: 20 281 651 1463
Fragments arranged by size:
1463 651 281 20
BtgI C'CryG_G
Cuts at: 0 543 2385 2415
Size: 543 1842 30
Fragments arranged by size:
1842 543 30
DraIII CAC_nnn'GTG
Cuts at: 0 731 2415
Size: 731 1684
DrdI GACnn_nn'nnGTC
Cuts at: 0 195 433 916 1174 2415
Size: 195 238 483 258 1241
Fragments arranged by size:
1241 483 258 238 195
EaeI y'GGCC_r
Cuts at: 0 1670 1916 2415
Size: 1670 246 499
Fragments arranged by size:
1670 499 246
EarI CTCTTCn'nnn_
Cuts at: 0 412 1100 1342 1994 2348 2415
Size: 412 688 242 652 354 67
Fragments arranged by size:
688 652 412 354 242 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 2415
Size: 478 1937
EcoICRI GAG'CTC
Cuts at: 0 405 1119 1911 2415
Size: 405 714 792 504
Fragments arranged by size:
792 714 504 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 121 179 354 452 668 847 1135
Size: 105 16 58 175 98 216 179 288
Cuts at: 1135 2014 2170 2233 2415
Size: 879 156 63 182
Fragments arranged by size:
879 288 216 182 179 175 156 105
98 63 58 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 1753 2415
Size: 865 888 662
Fragments arranged by size:
888 865 662
EcoO109I rG'GnC_Cy
Cuts at: 0 130 188 486 713 858 1499 1609 1821
Size: 130 58 298 227 145 641 110 212
Cuts at: 1821 1989 2415
Size: 168 426
Fragments arranged by size:
641 426 298 227 212 168 145 130
110 58
EcoRI G'AATT_C
Cuts at: 0 2116 2415
Size: 2116 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 974 1006 2415
Size: 664 32 278 32 1409
Fragments arranged by size:
1409 664 278 32 32
HincII GTy'rAC
Cuts at: 0 1332 2415
Size: 1332 1083
MscI TGG'CCA
Cuts at: 0 1672 2415
Size: 1672 743
MslI CAynn'nnrTG
Cuts at: 0 391 895 2415
Size: 391 504 1520
Fragments arranged by size:
1520 504 391
MspA1I CmG'CkG
Cuts at: 0 203 268 797 1415 2148 2290 2415
Size: 203 65 529 618 733 142 125
Fragments arranged by size:
733 618 529 203 142 125 65
NcoI C'CATG_G
Cuts at: 0 543 2415
Size: 543 1872
NspI r_CATG'y
Cuts at: 0 579 1665 2127 2415
Size: 579 1086 462 288
Fragments arranged by size:
1086 579 462 288
PasI CC'CwG_GG
Cuts at: 0 483 2306 2415
Size: 483 1823 109
Fragments arranged by size:
1823 483 109
PciI A'CATG_T
Cuts at: 0 1661 2415
Size: 1661 754
PflMI CCAn_nnn'nTGG
Cuts at: 0 537 2415
Size: 537 1878
PfoI T'CCnGG_A
Cuts at: 0 647 822 1112 2159 2415
Size: 647 175 290 1047 256
Fragments arranged by size:
1047 647 290 256 175
PpuMI rG'GwC_Cy
Cuts at: 0 188 1499 1821 1989 2415
Size: 188 1311 322 168 426
Fragments arranged by size:
1311 426 322 188 168
PshAI GACnn'nnGTC
Cuts at: 0 341 2415
Size: 341 2074
PspOMI G'GGCC_C
Cuts at: 0 486 712 857 1608 2415
Size: 486 226 145 751 807
Fragments arranged by size:
807 751 486 226 145
PspXI vC'TCGA_Gb
Cuts at: 0 893 1225 2415
Size: 893 332 1190
Fragments arranged by size:
1190 893 332
PstI C_TGCA'G
Cuts at: 0 1259 1829 2147 2415
Size: 1259 570 318 268
Fragments arranged by size:
1259 570 318 268
PvuII CAG'CTG
Cuts at: 0 203 268 2148 2290 2415
Size: 203 65 1880 142 125
Fragments arranged by size:
1880 203 142 125 65
SacI G_AGCT'C
Cuts at: 0 407 1121 1913 2415
Size: 407 714 792 502
Fragments arranged by size:
792 714 502 407
SanDI GG'GwC_CC
Cuts at: 0 1499 2415
Size: 1499 916
SapI GCTCTTCn'nnn_
Cuts at: 0 412 2415
Size: 412 2003
SbfI CC_TGCA'GG
Cuts at: 0 1829 2415
Size: 1829 586
SexAI A'CCwGG_T
Cuts at: 0 1169 2415
Size: 1169 1246
SfcI C'TryA_G
Cuts at: 0 72 792 977 1255 1825 2143 2189 2415
Size: 72 720 185 278 570 318 46 226
Fragments arranged by size:
720 570 318 278 226 185 72 46
SmlI C'TyrA_G
Cuts at: 0 893 1225 1872 2415
Size: 893 332 647 543
Fragments arranged by size:
893 647 543 332
StyI C'CwwG_G
Cuts at: 0 543 1421 2415
Size: 543 878 994
Fragments arranged by size:
994 878 543
TatI w'GTAC_w
Cuts at: 0 1310 1364 2415
Size: 1310 54 1051
Fragments arranged by size:
1310 1051 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 859 891 2415
Size: 859 32 1524
Fragments arranged by size:
1524 859 32
Tth111I GACn'n_nGTC
Cuts at: 0 1784 2415
Size: 1784 631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 1197 1428 1602 2415
Size: 456 741 231 174 813
Fragments arranged by size:
813 741 456 231 174
XhoI C'TCGA_G
Cuts at: 0 893 1225 2415
Size: 893 332 1190
Fragments arranged by size:
1190 893 332
XmnI GAAnn'nnTTC
Cuts at: 0 1663 2415
Size: 1663 752
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AleI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI
Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SmlI StyI TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaHI BsiEI BsiWI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI EagI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SacII SalI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
XbaI XmaI ZraI