Restriction map for
pFN21AE5708
(Linear) (Six-base) MAPSORT of: KIEE5708.seq Check: 6279 from: 1 to: 2211
KIEE5708.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 998 1151 2106 2115 2139 2211
Size: 998 153 955 9 24 72
Fragments arranged by size:
998 955 153 72 24 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 455 968 1184 1284 1385 2211
Size: 242 213 513 216 100 101 826
Fragments arranged by size:
826 513 242 216 213 101 100
AflIII A'CryG_T
Cuts at: 0 391 855 1536 2211
Size: 391 464 681 675
Fragments arranged by size:
681 675 464 391
AhdI GACnn_n'nnGTC
Cuts at: 0 503 2211
Size: 503 1708
AlwNI CAG_nnn'CTG
Cuts at: 0 1653 2211
Size: 1653 558
ApoI r'AATT_y
Cuts at: 0 250 2211
Size: 250 1961
AseI AT'TA_AT
Cuts at: 0 758 2211
Size: 758 1453
BanI G'GyrC_C
Cuts at: 0 107 2211
Size: 107 2104
BbsI GAAGACnn'nnnn_
Cuts at: 0 956 2160 2211
Size: 956 1204 51
Fragments arranged by size:
1204 956 51
BciVI GTATCCnnnnn_n'
Cuts at: 0 1952 2211
Size: 1952 259
BclI T'GATC_A
Cuts at: 0 1739 2211
Size: 1739 472
BfrBI ATG'CAT
Cuts at: 0 1681 2211
Size: 1681 530
BglI GCCn_nnn'nGGC
Cuts at: 0 2069 2211
Size: 2069 142
BglII A'GATC_T
Cuts at: 0 282 2211
Size: 282 1929
BmrI ACTGGGnnnn_n'
Cuts at: 0 1239 2211
Size: 1239 972
Bpu10I CC'TnA_GC
Cuts at: 0 2130 2211
Size: 2130 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 2211
Size: 521 1690
BsaI GGTCTCn'nnnn_
Cuts at: 0 376 1118 1183 1601 2211
Size: 376 742 65 418 610
Fragments arranged by size:
742 610 418 376 65
BsaAI yAC'GTr
Cuts at: 0 394 2211
Size: 394 1817
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 67 394 1382 1385 2049 2211
Size: 4 63 327 988 3 664 162
Fragments arranged by size:
988 664 327 162 63 4 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1691 2211
Size: 1691 520
BsiEI CG_ry'CG
Cuts at: 0 2097 2211
Size: 2097 114
BsmI GAATG_Cn'
Cuts at: 0 1245 2211
Size: 1245 966
BspHI T'CATG_A
Cuts at: 0 924 2211
Size: 924 1287
BsrBI CCG'CTC
Cuts at: 0 629 2211
Size: 629 1582
BsrDI GCAATG_nn'
Cuts at: 0 1262 2211
Size: 1262 949
BsrFI r'CCGG_y
Cuts at: 0 2063 2072 2096 2211
Size: 2063 9 24 115
Fragments arranged by size:
2063 115 24 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1678 1687 2211
Size: 1678 9 524
Fragments arranged by size:
1678 524 9
BstBI TT'CG_AA
Cuts at: 0 768 2211
Size: 768 1443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1426 1708 1773 2211
Size: 1426 282 65 438
Fragments arranged by size:
1426 438 282 65
BstYI r'GATC_y
Cuts at: 0 282 837 2211
Size: 282 555 1374
Fragments arranged by size:
1374 555 282
BstZ17I GTA'TAC
Cuts at: 0 999 2211
Size: 999 1212
Bsu36I CC'TnA_GG
Cuts at: 0 1397 2211
Size: 1397 814
BtgI C'CryG_G
Cuts at: 0 1347 2211
Size: 1347 864
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1405 2211
Size: 1405 806
BtsI GCAGTG_nn'
Cuts at: 0 1679 1853 2211
Size: 1679 174 358
Fragments arranged by size:
1679 358 174
ClaI AT'CG_AT
Cuts at: 0 2175 2211
Size: 2175 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1064 1099 1319 1354 1718 1753 2211
Size: 1064 35 220 35 364 35 458
Fragments arranged by size:
1064 458 364 220 35 35 35
DraI TTT'AAA
Cuts at: 0 1317 1533 2211
Size: 1317 216 678
Fragments arranged by size:
1317 678 216
DrdI GACnn_nn'nnGTC
Cuts at: 0 2123 2211
Size: 2123 88
EaeI y'GGCC_r
Cuts at: 0 828 1094 1214 2094 2211
Size: 828 266 120 880 117
Fragments arranged by size:
880 828 266 120 117
EagI C'GGCC_G
Cuts at: 0 2094 2211
Size: 2094 117
EarI CTCTTCn'nnn_
Cuts at: 0 431 1655 2211
Size: 431 1224 556
Fragments arranged by size:
1224 556 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 455 968 1184 1284 1385 2211
Size: 242 213 513 216 100 101 826
Fragments arranged by size:
826 513 242 216 213 101 100
EcoRI G'AATT_C
Cuts at: 0 250 2211
Size: 250 1961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 238 270 489 521 1393 1425
Size: 115 32 91 32 219 32 872 32
Cuts at: 1425 1724 1755 1756 1787 2211
Size: 299 31 1 31 424
Fragments arranged by size:
872 424 299 219 115 91 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 1044 1371 1495 2116 2140 2211
Size: 1044 327 124 621 24 71
Fragments arranged by size:
1044 621 327 124 71 24
HindIII A'AGCT_T
Cuts at: 0 448 1719 2211
Size: 448 1271 492
Fragments arranged by size:
1271 492 448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 771 1032 1659 1976 2211
Size: 679 92 261 627 317 235
Fragments arranged by size:
679 627 317 261 235 92
MscI TGG'CCA
Cuts at: 0 1096 1216 2211
Size: 1096 120 995
Fragments arranged by size:
1096 995 120
MslI CAynn'nnrTG
Cuts at: 0 113 2211
Size: 113 2098
MspA1I CmG'CkG
Cuts at: 0 1805 2211
Size: 1805 406
NsiI A_TGCA'T
Cuts at: 0 1683 2211
Size: 1683 528
NspI r_CATG'y
Cuts at: 0 859 2211
Size: 859 1352
PciI A'CATG_T
Cuts at: 0 855 2211
Size: 855 1356
PmlI CAC'GTG
Cuts at: 0 394 2211
Size: 394 1817
PstI C_TGCA'G
Cuts at: 0 824 1692 2211
Size: 824 868 519
Fragments arranged by size:
868 824 519
PvuII CAG'CTG
Cuts at: 0 1805 2211
Size: 1805 406
SalI G'TCGA_C
Cuts at: 0 2114 2138 2211
Size: 2114 24 73
Fragments arranged by size:
2114 73 24
SexAI A'CCwGG_T
Cuts at: 0 798 1373 2211
Size: 798 575 838
Fragments arranged by size:
838 798 575
SfcI C'TryA_G
Cuts at: 0 820 1152 1688 2211
Size: 820 332 536 523
Fragments arranged by size:
820 536 523 332
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2069 2211
Size: 2069 142
SmlI C'TyrA_G
Cuts at: 0 536 2211
Size: 536 1675
SspI AAT'ATT
Cuts at: 0 1999 2211
Size: 1999 212
StyI C'CwwG_G
Cuts at: 0 47 176 2211
Size: 47 129 2035
Fragments arranged by size:
2035 129 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1879 2211
Size: 1879 332
Tth111I GACn'n_nGTC
Cuts at: 0 2111 2211
Size: 2111 100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 823 1252 1596 2211
Size: 823 429 344 615
Fragments arranged by size:
823 615 429 344
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApoI AseI BanI
BbsI BciVI BclI BfrBI BglI BglII BmrI Bpu10I
BpuEI BsaI BsaAI BseRI BsgI BsiEI BsmI BspHI
BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI Eco57MI EcoRI Hin4I HincII HindIII
MmeI MscI MslI MspA1I NsiI NspI PciI PmlI
PstI PvuII SalI SexAI SfcI SfiI SmlI SspI
StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BlpI Bme1580I
BmgBI BmtI BplI BpmI BsaBI BsaHI BsaWI BsaXI
BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrGI
BssHII BssSI BstEII DraIII EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI