Restriction map for
pFN21AE5707
(Linear) (Six-base) MAPSORT of: KIEE5707.seq Check: 5453 from: 1 to: 1962
KIEE5707.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 1419 1962
Size: 1419 543
AclI AA'CG_TT
Cuts at: 0 740 1962
Size: 740 1222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 508 553 832 1850 1962
Size: 263 245 45 279 1018 112
Fragments arranged by size:
1018 279 263 245 112 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1073 1962
Size: 1073 889
ApoI r'AATT_y
Cuts at: 0 299 718 1327 1948 1962
Size: 299 419 609 621 14
Fragments arranged by size:
621 609 419 299 14
AseI AT'TA_AT
Cuts at: 0 1241 1962
Size: 1241 721
AvaI C'yCGr_G
Cuts at: 0 1403 1962
Size: 1403 559
BanI G'GyrC_C
Cuts at: 0 1822 1962
Size: 1822 140
BanII G_rGCy'C
Cuts at: 0 59 1962
Size: 59 1903
BbsI GAAGACnn'nnnn_
Cuts at: 0 1789 1962
Size: 1789 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 1157 1191 1962
Size: 283 34 840 34 771
Fragments arranged by size:
840 771 283 34 34
BfrBI ATG'CAT
Cuts at: 0 1060 1962
Size: 1060 902
BlpI GC'TnA_GC
Cuts at: 0 53 1962
Size: 53 1909
BmrI ACTGGGnnnn_n'
Cuts at: 0 419 738 791 1962
Size: 419 319 53 1171
Fragments arranged by size:
1171 419 319 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1285 1376 1962
Size: 712 573 91 586
Fragments arranged by size:
712 586 573 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 1962
Size: 70 1892
BsaHI Gr'CG_yC
Cuts at: 0 37 1962
Size: 37 1925
BsaWI w'CCGG_w
Cuts at: 0 161 594 1962
Size: 161 433 1368
Fragments arranged by size:
1368 433 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 1962
Size: 565 30 1367
Fragments arranged by size:
1367 565 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 221 1874 1962
Size: 15 206 1653 88
Fragments arranged by size:
1653 206 88 15
BseYI C'CCAG_C
Cuts at: 0 395 1889 1962
Size: 395 1494 73
Fragments arranged by size:
1494 395 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 1033 1962
Size: 768 265 929
Fragments arranged by size:
929 768 265
BsiEI CG_ry'CG
Cuts at: 0 11 47 1962
Size: 11 36 1915
Fragments arranged by size:
1915 36 11
BsiHKAI G_wGCw'C
Cuts at: 0 59 1962
Size: 59 1903
Bsp1286I G_dGCh'C
Cuts at: 0 59 1962
Size: 59 1903
BspEI T'CCGG_A
Cuts at: 0 161 1962
Size: 161 1801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 1962
Size: 390 1572
BstYI r'GATC_y
Cuts at: 0 154 471 1962
Size: 154 317 1491
Fragments arranged by size:
1491 317 154
Bsu36I CC'TnA_GG
Cuts at: 0 27 1962
Size: 27 1935
BtgI C'CryG_G
Cuts at: 0 47 1962
Size: 47 1915
ClaI AT'CG_AT
Cuts at: 0 870 1962
Size: 870 1092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 741 776 812 847 1962
Size: 741 35 36 35 1115
Fragments arranged by size:
1115 741 36 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 732 1962
Size: 732 1230
EaeI y'GGCC_r
Cuts at: 0 8 44 1962
Size: 8 36 1918
Fragments arranged by size:
1918 36 8
EagI C'GGCC_G
Cuts at: 0 8 44 1962
Size: 8 36 1918
Fragments arranged by size:
1918 36 8
EarI CTCTTCn'nnn_
Cuts at: 0 236 572 1181 1962
Size: 236 336 609 781
Fragments arranged by size:
781 609 336 236
EcoICRI GAG'CTC
Cuts at: 0 57 1962
Size: 57 1905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 508 553 712 832 1285 1376 1850
Size: 263 245 45 159 120 453 91 474
Cuts at: 1850 1962
Size: 112
Fragments arranged by size:
474 453 263 245 159 120 112 91
45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 203 1962
Size: 203 1759
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1243 1962
Size: 1243 719
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1657 1689 1962
Size: 463 32 1162 32 273
Fragments arranged by size:
1162 463 273 32 32
HindIII A'AGCT_T
Cuts at: 0 275 1114 1905 1962
Size: 275 839 791 57
Fragments arranged by size:
839 791 275 57
MfeI C'AATT_G
Cuts at: 0 939 1962
Size: 939 1023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1962
Size: 554 1408
MslI CAynn'nnrTG
Cuts at: 0 845 1055 1805 1962
Size: 845 210 750 157
Fragments arranged by size:
845 750 210 157
MspA1I CmG'CkG
Cuts at: 0 291 1041 1893 1962
Size: 291 750 852 69
Fragments arranged by size:
852 750 291 69
NotI GC'GGCC_GC
Cuts at: 0 8 1962
Size: 8 1954
NsiI A_TGCA'T
Cuts at: 0 1062 1962
Size: 1062 900
NspI r_CATG'y
Cuts at: 0 544 1064 1962
Size: 544 520 898
Fragments arranged by size:
898 544 520
PfoI T'CCnGG_A
Cuts at: 0 1654 1962
Size: 1654 308
PshAI GACnn'nnGTC
Cuts at: 0 1018 1962
Size: 1018 944
PsiI TTA'TAA
Cuts at: 0 1945 1962
Size: 1945 17
PstI C_TGCA'G
Cuts at: 0 387 1962
Size: 387 1575
PvuII CAG'CTG
Cuts at: 0 291 1893 1962
Size: 291 1602 69
Fragments arranged by size:
1602 291 69
SacI G_AGCT'C
Cuts at: 0 59 1962
Size: 59 1903
SapI GCTCTTCn'nnn_
Cuts at: 0 572 1962
Size: 572 1390
SfcI C'TryA_G
Cuts at: 0 383 965 1420 1962
Size: 383 582 455 542
Fragments arranged by size:
582 542 455 383
SspI AAT'ATT
Cuts at: 0 1494 1962
Size: 1494 468
StuI AGG'CCT
Cuts at: 0 91 1962
Size: 91 1871
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 720 1903 1962
Size: 720 1183 59
Fragments arranged by size:
1183 720 59
Tth111I GACn'n_nGTC
Cuts at: 0 771 1006 1962
Size: 771 235 956
Fragments arranged by size:
956 771 235
XbaI T'CTAG_A
Cuts at: 0 513 775 1962
Size: 513 262 1187
Fragments arranged by size:
1187 513 262
XmnI GAAnn'nnTTC
Cuts at: 0 521 722 1962
Size: 521 201 1240
Fragments arranged by size:
1240 521 201
Enzymes that do cut:
AccI AclI AcuI AlfI ApoI AseI AvaI BanI
BanII BbsI BcgI BfrBI BlpI BmrI BpmI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BstYI Bsu36I BtgI ClaI CspCI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI FalI
Hin4I HindIII MfeI MmeI MslI MspA1I NotI NsiI
NspI PfoI PshAI PsiI PstI PvuII SacI SapI
SfcI SspI StuI TaqII Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BglI BglII
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI DraI DrdI EciI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StyI SwaI TatI
TstI XcmI XhoI XmaI ZraI