Restriction map for
pFN21AE5704
(Linear) (Six-base) MAPSORT of: KIEE5704.seq Check: 9059 from: 1 to: 1986
KIEE5704.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 670 762 1986
Size: 670 92 1224
Fragments arranged by size:
1224 670 92
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 986 1986
Size: 562 424 1000
Fragments arranged by size:
1000 562 424
AlwNI CAG_nnn'CTG
Cuts at: 0 1801 1986
Size: 1801 185
ApoI r'AATT_y
Cuts at: 0 613 949 1207 1259 1821 1930 1986
Size: 613 336 258 52 562 109 56
Fragments arranged by size:
613 562 336 258 109 56 52
BanII G_rGCy'C
Cuts at: 0 693 1986
Size: 693 1293
BbvCI CC'TCA_GC
Cuts at: 0 1283 1986
Size: 1283 703
BciVI GTATCCnnnnn_n'
Cuts at: 0 1607 1986
Size: 1607 379
BclI T'GATC_A
Cuts at: 0 701 1986
Size: 701 1285
BfrBI ATG'CAT
Cuts at: 0 1560 1986
Size: 1560 426
Bme1580I G_kGCm'C
Cuts at: 0 753 1236 1986
Size: 753 483 750
Fragments arranged by size:
753 750 483
BmrI ACTGGGnnnn_n'
Cuts at: 0 763 1145 1986
Size: 763 382 841
Fragments arranged by size:
841 763 382
BmtI G_CTAG'C
Cuts at: 0 1651 1986
Size: 1651 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1986
Size: 1297 689
Bpu10I CC'TnA_GC
Cuts at: 0 1283 1751 1986
Size: 1283 468 235
Fragments arranged by size:
1283 468 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1688 1986
Size: 1688 298
BsaWI w'CCGG_w
Cuts at: 0 903 1986
Size: 903 1083
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 629 659 1131 1161 1334 1364 1986
Size: 629 30 472 30 173 30 622
Fragments arranged by size:
629 622 472 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1271 1764 1986
Size: 1271 493 222
Fragments arranged by size:
1271 493 222
BsiEI CG_ry'CG
Cuts at: 0 137 141 1986
Size: 137 4 1845
Fragments arranged by size:
1845 137 4
BsmI GAATG_Cn'
Cuts at: 0 1560 1986
Size: 1560 426
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1670 1986
Size: 1670 316
Bsp1286I G_dGCh'C
Cuts at: 0 693 753 1236 1986
Size: 693 60 483 750
Fragments arranged by size:
750 693 483 60
BspEI T'CCGG_A
Cuts at: 0 903 1986
Size: 903 1083
BsrBI CCG'CTC
Cuts at: 0 52 1986
Size: 52 1934
BsrDI GCAATG_nn'
Cuts at: 0 870 1814 1986
Size: 870 944 172
Fragments arranged by size:
944 870 172
BsrFI r'CCGG_y
Cuts at: 0 88 1986
Size: 88 1898
BssSI C'ACGA_G
Cuts at: 0 199 1986
Size: 199 1787
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1536 1986
Size: 1536 450
BstYI r'GATC_y
Cuts at: 0 537 900 1986
Size: 537 363 1086
Fragments arranged by size:
1086 537 363
BstZ17I GTA'TAC
Cuts at: 0 671 1986
Size: 671 1315
BtgI C'CryG_G
Cuts at: 0 113 1986
Size: 113 1873
BtsI GCAGTG_nn'
Cuts at: 0 606 1986
Size: 606 1380
DraI TTT'AAA
Cuts at: 0 948 1986
Size: 948 1038
EaeI y'GGCC_r
Cuts at: 0 138 170 1986
Size: 138 32 1816
Fragments arranged by size:
1816 138 32
EagI C'GGCC_G
Cuts at: 0 138 1986
Size: 138 1848
EarI CTCTTCn'nnn_
Cuts at: 0 155 794 1013 1904 1986
Size: 155 639 219 891 82
Fragments arranged by size:
891 639 219 155 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 62 1986
Size: 62 1924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 986 1297 1986
Size: 562 424 311 689
Fragments arranged by size:
689 562 424 311
EcoO109I rG'GnC_Cy
Cuts at: 0 1276 1986
Size: 1276 710
EcoRI G'AATT_C
Cuts at: 0 1207 1986
Size: 1207 779
EcoRV GAT'ATC
Cuts at: 0 744 1986
Size: 744 1242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1977 1986
Size: 1977 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 749 781 1635 1667 1879 1911 1986
Size: 749 32 854 32 212 32 75
Fragments arranged by size:
854 749 212 75 32 32 32
HincII GTy'rAC
Cuts at: 0 1904 1986
Size: 1904 82
HpaI GTT'AAC
Cuts at: 0 1904 1986
Size: 1904 82
MfeI C'AATT_G
Cuts at: 0 1100 1986
Size: 1100 886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1001 1737 1986
Size: 8 993 736 249
Fragments arranged by size:
993 736 249 8
MslI CAynn'nnrTG
Cuts at: 0 756 1986
Size: 756 1230
MspA1I CmG'CkG
Cuts at: 0 41 1039 1986
Size: 41 998 947
Fragments arranged by size:
998 947 41
NaeI GCC'GGC
Cuts at: 0 90 1986
Size: 90 1896
NgoMIV G'CCGG_C
Cuts at: 0 88 1986
Size: 88 1898
NheI G'CTAG_C
Cuts at: 0 1647 1986
Size: 1647 339
NsiI A_TGCA'T
Cuts at: 0 1562 1986
Size: 1562 424
NspI r_CATG'y
Cuts at: 0 258 1519 1986
Size: 258 1261 467
Fragments arranged by size:
1261 467 258
PpuMI rG'GwC_Cy
Cuts at: 0 1276 1986
Size: 1276 710
PvuII CAG'CTG
Cuts at: 0 1039 1986
Size: 1039 947
SapI GCTCTTCn'nnn_
Cuts at: 0 1013 1986
Size: 1013 973
ScaI AGT'ACT
Cuts at: 0 1310 1986
Size: 1310 676
SexAI A'CCwGG_T
Cuts at: 0 146 1986
Size: 146 1840
SfcI C'TryA_G
Cuts at: 0 436 1321 1779 1986
Size: 436 885 458 207
Fragments arranged by size:
885 458 436 207
SmlI C'TyrA_G
Cuts at: 0 1703 1986
Size: 1703 283
SspI AAT'ATT
Cuts at: 0 321 394 555 1986
Size: 321 73 161 1431
Fragments arranged by size:
1431 321 161 73
StyI C'CwwG_G
Cuts at: 0 734 1986
Size: 734 1252
SwaI ATTT'AAAT
Cuts at: 0 948 1986
Size: 948 1038
TatI w'GTAC_w
Cuts at: 0 582 1308 1986
Size: 582 726 678
Fragments arranged by size:
726 678 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 1986
Size: 398 32 1556
Fragments arranged by size:
1556 398 32
XmnI GAAnn'nnTTC
Cuts at: 0 7 1986
Size: 7 1979
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanII BbvCI BciVI BclI
BfrBI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BsiEI BsmI BsmBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI BstZ17I BtgI BtsI
DraI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HpaI MfeI MmeI MslI
MspA1I NaeI NgoMIV NheI NsiI NspI PpuMI PvuII
SapI ScaI SexAI SfcI SmlI SspI StyI SwaI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BglI BglII BlpI BmgBI BplI BsaI BsaAI
BsaBI BsaHI BseYI BsgI BsiHKAI BsiWI BspHI BspMI
BsrGI BssHII BstBI BstEII BstXI Bsu36I BtgZI ClaI
CspCI DraIII DrdI EcoICRI EcoNI FseI FspI FspAI
HaeII HindIII KasI KpnI MluI MscI NarI NcoI
NdeI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI