Restriction map for
pFN21AE5703
(Linear) (Six-base) MAPSORT of: KIEE5703.seq Check: 211 from: 1 to: 1941
KIEE5703.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AatII G_ACGT'C
Cuts at: 0 1117 1941
Size: 1117 824
AccI GT'mk_AC
Cuts at: 0 869 1941
Size: 869 1072
Acc65I G'GTAC_C
Cuts at: 0 1511 1941
Size: 1511 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 1285 1348 1619 1762 1795 1840 1941
Size: 909 376 63 271 143 33 45 101
Fragments arranged by size:
909 376 271 143 101 63 45 33
AfeI AGC'GCT
Cuts at: 0 44 1369 1941
Size: 44 1325 572
Fragments arranged by size:
1325 572 44
AflII C'TTAA_G
Cuts at: 0 910 1941
Size: 910 1031
AlwNI CAG_nnn'CTG
Cuts at: 0 91 288 859 1236 1818 1941
Size: 91 197 571 377 582 123
Fragments arranged by size:
582 571 377 197 123 91
ApaI G_GGCC'C
Cuts at: 0 235 1417 1426 1941
Size: 235 1182 9 515
Fragments arranged by size:
1182 515 235 9
ApaLI G'TGCA_C
Cuts at: 0 1387 1941
Size: 1387 554
ApoI r'AATT_y
Cuts at: 0 409 1941
Size: 409 1532
AvaI C'yCGr_G
Cuts at: 0 228 304 469 497 647 1651 1839 1941
Size: 228 76 165 28 150 1004 188 102
Fragments arranged by size:
1004 228 188 165 150 102 76 28
BamHI G'GATC_C
Cuts at: 0 1487 1941
Size: 1487 454
BanI G'GyrC_C
Cuts at: 0 312 827 1511 1941
Size: 312 515 684 430
Fragments arranged by size:
684 515 430 312
BanII G_rGCy'C
Cuts at: 0 235 1015 1286 1417 1426 1504 1941
Size: 235 780 271 131 9 78 437
Fragments arranged by size:
780 437 271 235 131 78 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1569 1941
Size: 1569 372
BbvCI CC'TCA_GC
Cuts at: 0 416 1941
Size: 416 1525
BglI GCCn_nnn'nGGC
Cuts at: 0 706 1421 1941
Size: 706 715 520
Fragments arranged by size:
715 706 520
BglII A'GATC_T
Cuts at: 0 1472 1941
Size: 1472 469
BlpI GC'TnA_GC
Cuts at: 0 4 1503 1941
Size: 4 1499 438
Fragments arranged by size:
1499 438 4
Bme1580I G_kGCm'C
Cuts at: 0 235 315 594 830 1391 1417 1426 1941
Size: 235 80 279 236 561 26 9 515
Fragments arranged by size:
561 515 279 236 235 80 26 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 550 697 1796 1941
Size: 550 147 1099 145
Fragments arranged by size:
1099 550 147 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 1941
Size: 61 1880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1072 1116 1720 1941
Size: 506 566 44 604 221
Fragments arranged by size:
604 566 506 221 44
Bpu10I CC'TnA_GC
Cuts at: 0 416 1285 1295 1941
Size: 416 869 10 646
Fragments arranged by size:
869 646 416 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 494 1534 1640 1924 1941
Size: 494 1040 106 284 17
Fragments arranged by size:
1040 494 284 106 17
BsaBI GATnn'nnATC
Cuts at: 0 385 1941
Size: 385 1556
BsaHI Gr'CG_yC
Cuts at: 0 1114 1758 1941
Size: 1114 644 183
Fragments arranged by size:
1114 644 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 198 228 1941
Size: 198 30 1713
Fragments arranged by size:
1713 198 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 433 457 1093 1171 1941
Size: 154 279 24 636 78 770
Fragments arranged by size:
770 636 279 154 78 24
BseYI C'CCAG_C
Cuts at: 0 16 202 364 696 1021 1179 1250 1641
Size: 16 186 162 332 325 158 71 391
Cuts at: 1641 1941
Size: 300
Fragments arranged by size:
391 332 325 300 186 162 158 71
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1941
Size: 1159 782
BsiEI CG_ry'CG
Cuts at: 0 137 389 1941
Size: 137 252 1552
Fragments arranged by size:
1552 252 137
BsiHKAI G_wGCw'C
Cuts at: 0 1015 1286 1391 1941
Size: 1015 271 105 550
Fragments arranged by size:
1015 550 271 105
BsmI GAATG_Cn'
Cuts at: 0 753 1941
Size: 753 1188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 1105 1941
Size: 124 981 836
Fragments arranged by size:
981 836 124
Bsp1286I G_dGCh'C
Cuts at: 0 235 315 594 830 1015 1286 1391 1417
Size: 235 80 279 236 185 271 105 26
Cuts at: 1417 1426 1504 1941
Size: 9 78 437
Fragments arranged by size:
437 279 271 236 235 185 105 80
78 26 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1094 1117 1253 1941
Size: 1094 23 136 688
Fragments arranged by size:
1094 688 136 23
BsrBI CCG'CTC
Cuts at: 0 8 13 1855 1941
Size: 8 5 1842 86
Fragments arranged by size:
1842 86 8 5
BsrDI GCAATG_nn'
Cuts at: 0 686 1941
Size: 686 1255
BsrFI r'CCGG_y
Cuts at: 0 1334 1941
Size: 1334 607
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 1941
Size: 288 1653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 637 1941
Size: 637 1304
BstYI r'GATC_y
Cuts at: 0 1142 1472 1487 1703 1941
Size: 1142 330 15 216 238
Fragments arranged by size:
1142 330 238 216 15
Bsu36I CC'TnA_GG
Cuts at: 0 1418 1941
Size: 1418 523
BtgI C'CryG_G
Cuts at: 0 567 1006 1478 1604 1902 1941
Size: 567 439 472 126 298 39
Fragments arranged by size:
567 472 439 298 126 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 782 1941
Size: 782 1159
BtsI GCAGTG_nn'
Cuts at: 0 295 936 1050 1374 1941
Size: 295 641 114 324 567
Fragments arranged by size:
641 567 324 295 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 909 944 1941
Size: 909 35 997
Fragments arranged by size:
997 909 35
DraIII CAC_nnn'GTG
Cuts at: 0 675 1941
Size: 675 1266
DrdI GACnn_nn'nnGTC
Cuts at: 0 1215 1941
Size: 1215 726
EaeI y'GGCC_r
Cuts at: 0 356 506 788 888 1941
Size: 356 150 282 100 1053
Fragments arranged by size:
1053 356 282 150 100
EarI CTCTTCn'nnn_
Cuts at: 0 259 272 1941
Size: 259 13 1669
Fragments arranged by size:
1669 259 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 50 933 1941
Size: 50 883 1008
Fragments arranged by size:
1008 883 50
EcoICRI GAG'CTC
Cuts at: 0 1013 1284 1941
Size: 1013 271 657
Fragments arranged by size:
1013 657 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 909 1072 1116 1285 1348 1619 1720
Size: 506 403 163 44 169 63 271 101
Cuts at: 1720 1762 1795 1840 1941
Size: 42 33 45 101
Fragments arranged by size:
506 403 271 169 163 101 101 63
45 44 42 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 1941
Size: 628 1313
EcoO109I rG'GnC_Cy
Cuts at: 0 232 562 1413 1414 1422 1587 1941
Size: 232 330 851 1 8 165 354
Fragments arranged by size:
851 354 330 232 165 8 1
FspI TGC'GCA
Cuts at: 0 577 1941
Size: 577 1364
HaeII r_GCGC'y
Cuts at: 0 46 1371 1941
Size: 46 1325 570
Fragments arranged by size:
1325 570 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 1941
Size: 651 32 1258
Fragments arranged by size:
1258 651 32
KpnI G_GTAC'C
Cuts at: 0 1515 1941
Size: 1515 426
MscI TGG'CCA
Cuts at: 0 358 508 790 890 1941
Size: 358 150 282 100 1051
Fragments arranged by size:
1051 358 282 150 100
MspA1I CmG'CkG
Cuts at: 0 98 171 288 691 1008 1179 1233 1941
Size: 98 73 117 403 317 171 54 708
Fragments arranged by size:
708 403 317 171 117 98 73 54
NaeI GCC'GGC
Cuts at: 0 1336 1941
Size: 1336 605
NcoI C'CATG_G
Cuts at: 0 1478 1604 1941
Size: 1478 126 337
Fragments arranged by size:
1478 337 126
NgoMIV G'CCGG_C
Cuts at: 0 1334 1941
Size: 1334 607
NspI r_CATG'y
Cuts at: 0 909 1395 1941
Size: 909 486 546
Fragments arranged by size:
909 546 486
PasI CC'CwG_GG
Cuts at: 0 1256 1404 1941
Size: 1256 148 537
Fragments arranged by size:
1256 537 148
PflMI CCAn_nnn'nTGG
Cuts at: 0 1179 1941
Size: 1179 762
PspOMI G'GGCC_C
Cuts at: 0 231 1413 1422 1941
Size: 231 1182 9 519
Fragments arranged by size:
1182 519 231 9
PvuI CG_AT'CG
Cuts at: 0 137 389 1941
Size: 137 252 1552
Fragments arranged by size:
1552 252 137
PvuII CAG'CTG
Cuts at: 0 98 1179 1941
Size: 98 1081 762
Fragments arranged by size:
1081 762 98
SacI G_AGCT'C
Cuts at: 0 1015 1286 1941
Size: 1015 271 655
Fragments arranged by size:
1015 655 271
SacII CC_GC'GG
Cuts at: 0 1009 1941
Size: 1009 932
SapI GCTCTTCn'nnn_
Cuts at: 0 272 1941
Size: 272 1669
SexAI A'CCwGG_T
Cuts at: 0 671 1941
Size: 671 1270
SfcI C'TryA_G
Cuts at: 0 1317 1941
Size: 1317 624
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1421 1941
Size: 1421 520
SmaI CCC'GGG
Cuts at: 0 649 1941
Size: 649 1292
SmlI C'TyrA_G
Cuts at: 0 910 1941
Size: 910 1031
StyI C'CwwG_G
Cuts at: 0 434 1478 1604 1941
Size: 434 1044 126 337
Fragments arranged by size:
1044 434 337 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1396 1941
Size: 1396 545
TatI w'GTAC_w
Cuts at: 0 1526 1941
Size: 1526 415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 1941
Size: 198 32 1711
Fragments arranged by size:
1711 198 32
XmaI C'CCGG_G
Cuts at: 0 647 1941
Size: 647 1294
ZraI GAC'GTC
Cuts at: 0 1115 1941
Size: 1115 826
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflII AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KpnI MscI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PspOMI PvuI PvuII
SacI SacII SapI SexAI SfcI SfiI SmaI SmlI
StyI TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflIII AgeI AhdI AleI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BmgBI BmtI BpuEI BsaAI BsaWI BsiWI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI DraI EagI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI MslI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI RsrII SalI SanDI SbfI ScaI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmnI