Restriction map for
pFN21AE5692
(Linear) (Six-base) MAPSORT of: KIEE5692.seq Check: 2687 from: 1 to: 1503
KIEE5692.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AatII G_ACGT'C
Cuts at: 0 460 1503
Size: 460 1043
AccI GT'mk_AC
Cuts at: 0 650 1503
Size: 650 853
Acc65I G'GTAC_C
Cuts at: 0 112 798 1503
Size: 112 686 705
Fragments arranged by size:
705 686 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 343 760 916 1117 1503
Size: 190 153 417 156 201 386
Fragments arranged by size:
417 386 201 190 156 153
AfeI AGC'GCT
Cuts at: 0 1435 1503
Size: 1435 68
AhdI GACnn_n'nnGTC
Cuts at: 0 438 1503
Size: 438 1065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 995 1503
Size: 995 508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1503
Size: 1108 32 363
Fragments arranged by size:
1108 363 32
AlwNI CAG_nnn'CTG
Cuts at: 0 488 585 758 783 945 1503
Size: 488 97 173 25 162 558
Fragments arranged by size:
558 488 173 162 97 25
ApaI G_GGCC'C
Cuts at: 0 882 1503
Size: 882 621
ApaLI G'TGCA_C
Cuts at: 0 1168 1503
Size: 1168 335
ApoI r'AATT_y
Cuts at: 0 163 1503
Size: 163 1340
AvaI C'yCGr_G
Cuts at: 0 668 1503
Size: 668 835
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 96 129 1503
Size: 96 33 1374
Fragments arranged by size:
1374 96 33
BanI G'GyrC_C
Cuts at: 0 112 798 994 1447 1503
Size: 112 686 196 453 56
Fragments arranged by size:
686 453 196 112 56
BanII G_rGCy'C
Cuts at: 0 30 98 882 1199 1503
Size: 30 68 784 317 304
Fragments arranged by size:
784 317 304 68 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1091 1197 1503
Size: 1091 106 306
Fragments arranged by size:
1091 306 106
BbvCI CC'TCA_GC
Cuts at: 0 804 1048 1503
Size: 804 244 455
Fragments arranged by size:
804 455 244
BciVI GTATCCnnnnn_n'
Cuts at: 0 43 328 1503
Size: 43 285 1175
Fragments arranged by size:
1175 285 43
Bme1580I G_kGCm'C
Cuts at: 0 122 882 999 1172 1503
Size: 122 760 117 173 331
Fragments arranged by size:
760 331 173 122 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 1006 1503
Size: 1006 497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 679 982 1503
Size: 489 190 303 521
Fragments arranged by size:
521 489 303 190
Bpu10I CC'TnA_GC
Cuts at: 0 547 804 1048 1193 1503
Size: 547 257 244 145 310
Fragments arranged by size:
547 310 257 244 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1503
Size: 253 1250
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 958 1503
Size: 415 543 545
Fragments arranged by size:
545 543 415
BsaHI Gr'CG_yC
Cuts at: 0 457 1503
Size: 457 1046
BsaWI w'CCGG_w
Cuts at: 0 146 1503
Size: 146 1357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 1049 1079 1108 1138 1503
Size: 235 30 784 30 29 30 365
Fragments arranged by size:
784 365 235 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1048 1057 1503
Size: 1048 9 446
Fragments arranged by size:
1048 446 9
BseYI C'CCAG_C
Cuts at: 0 21 762 1436 1459 1503
Size: 21 741 674 23 44
Fragments arranged by size:
741 674 44 23 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1040 1503
Size: 229 811 463
Fragments arranged by size:
811 463 229
BsiEI CG_ry'CG
Cuts at: 0 1218 1503
Size: 1218 285
BsiHKAI G_wGCw'C
Cuts at: 0 30 1172 1503
Size: 30 1142 331
Fragments arranged by size:
1142 331 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 1030 1503
Size: 706 324 473
Fragments arranged by size:
706 473 324
Bsp1286I G_dGCh'C
Cuts at: 0 30 98 122 882 999 1172 1199 1503
Size: 30 68 24 760 117 173 27 304
Fragments arranged by size:
760 304 173 117 68 30 27 24
BspEI T'CCGG_A
Cuts at: 0 146 1503
Size: 146 1357
BspHI T'CATG_A
Cuts at: 0 830 1080 1503
Size: 830 250 423
Fragments arranged by size:
830 423 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 1503
Size: 593 910
BsrDI GCAATG_nn'
Cuts at: 0 41 1503
Size: 41 1462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1503
Size: 585 918
BstYI r'GATC_y
Cuts at: 0 411 1274 1503
Size: 411 863 229
Fragments arranged by size:
863 411 229
Bsu36I CC'TnA_GG
Cuts at: 0 1069 1503
Size: 1069 434
BtgI C'CryG_G
Cuts at: 0 88 137 1131 1503
Size: 88 49 994 372
Fragments arranged by size:
994 372 88 49
DraIII CAC_nnn'GTG
Cuts at: 0 1342 1470 1503
Size: 1342 128 33
Fragments arranged by size:
1342 128 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 698 972 1503
Size: 698 274 531
Fragments arranged by size:
698 531 274
EaeI y'GGCC_r
Cuts at: 0 129 1503
Size: 129 1374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1117 1503
Size: 1117 386
EcoICRI GAG'CTC
Cuts at: 0 28 1503
Size: 28 1475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 343 489 679 760 916 982 1117
Size: 190 153 146 190 81 156 66 135
Cuts at: 1117 1503
Size: 386
Fragments arranged by size:
386 190 190 156 153 146 135 81
66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 589 949 1503
Size: 589 360 554
Fragments arranged by size:
589 554 360
EcoO109I rG'GnC_Cy
Cuts at: 0 582 862 879 942 1503
Size: 582 280 17 63 561
Fragments arranged by size:
582 561 280 63 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 843 993 1503
Size: 843 150 510
Fragments arranged by size:
843 510 150
HaeII r_GCGC'y
Cuts at: 0 1437 1503
Size: 1437 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 424 456 1503
Size: 235 32 157 32 1047
Fragments arranged by size:
1047 235 157 32 32
HincII GTy'rAC
Cuts at: 0 1425 1503
Size: 1425 78
KpnI G_GTAC'C
Cuts at: 0 116 802 1503
Size: 116 686 701
Fragments arranged by size:
701 686 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1503
Size: 99 1404
MscI TGG'CCA
Cuts at: 0 131 1503
Size: 131 1372
MslI CAynn'nnrTG
Cuts at: 0 194 1503
Size: 194 1309
MspA1I CmG'CkG
Cuts at: 0 538 1503
Size: 538 965
NcoI C'CATG_G
Cuts at: 0 88 137 1131 1503
Size: 88 49 994 372
Fragments arranged by size:
994 372 88 49
NspI r_CATG'y
Cuts at: 0 774 1503
Size: 774 729
PasI CC'CwG_GG
Cuts at: 0 849 1295 1321 1503
Size: 849 446 26 182
Fragments arranged by size:
849 446 182 26
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 467 488 945 1295 1503
Size: 138 329 21 457 350 208
Fragments arranged by size:
457 350 329 208 138 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1503
Size: 1108 32 363
Fragments arranged by size:
1108 363 32
PpuMI rG'GwC_Cy
Cuts at: 0 582 942 1503
Size: 582 360 561
Fragments arranged by size:
582 561 360
PspOMI G'GGCC_C
Cuts at: 0 878 1503
Size: 878 625
PvuI CG_AT'CG
Cuts at: 0 1218 1503
Size: 1218 285
PvuII CAG'CTG
Cuts at: 0 538 1503
Size: 538 965
SacI G_AGCT'C
Cuts at: 0 30 1503
Size: 30 1473
ScaI AGT'ACT
Cuts at: 0 226 1503
Size: 226 1277
SfcI C'TryA_G
Cuts at: 0 589 1496 1503
Size: 589 907 7
Fragments arranged by size:
907 589 7
SmaI CCC'GGG
Cuts at: 0 670 1503
Size: 670 833
SmlI C'TyrA_G
Cuts at: 0 268 1503
Size: 268 1235
StyI C'CwwG_G
Cuts at: 0 88 137 640 1131 1503
Size: 88 49 503 491 372
Fragments arranged by size:
503 491 372 88 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 917 937 1074 1503
Size: 917 20 137 429
Fragments arranged by size:
917 429 137 20
TatI w'GTAC_w
Cuts at: 0 224 1503
Size: 224 1279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1503
Size: 1049 32 422
Fragments arranged by size:
1049 422 32
XbaI T'CTAG_A
Cuts at: 0 1017 1503
Size: 1017 486
XmaI C'CCGG_G
Cuts at: 0 668 1503
Size: 668 835
XmnI GAAnn'nnTTC
Cuts at: 0 911 1503
Size: 911 592
ZraI GAC'GTC
Cuts at: 0 458 1503
Size: 458 1045
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI
BstYI Bsu36I BtgI DraIII DrdI EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PpiI PpuMI PspOMI PvuI PvuII SacI ScaI SfcI
SmaI SmlI StyI TaqII TatI TstI XbaI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AleI AscI AseI
AsiSI AvrII BamHI BbeI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsiWI
BsmI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI EagI
EarI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI PstI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XcmI XhoI