Restriction map for
pFN21AE5683
(Linear) (Six-base) MAPSORT of: KIEE5683.seq Check: 5470 from: 1 to: 1656
KIEE5683.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AclI AA'CG_TT
Cuts at: 0 1546 1656
Size: 1546 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 576 1656
Size: 384 192 1080
Fragments arranged by size:
1080 384 192
AflII C'TTAA_G
Cuts at: 0 294 1656
Size: 294 1362
AflIII A'CryG_T
Cuts at: 0 507 1328 1656
Size: 507 821 328
Fragments arranged by size:
821 507 328
ApoI r'AATT_y
Cuts at: 0 907 1205 1656
Size: 907 298 451
Fragments arranged by size:
907 451 298
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 522 555 1656
Size: 522 33 1101
Fragments arranged by size:
1101 522 33
BanI G'GyrC_C
Cuts at: 0 148 1656
Size: 148 1508
BbsI GAAGACnn'nnnn_
Cuts at: 0 1293 1656
Size: 1293 363
BbvCI CC'TCA_GC
Cuts at: 0 763 1656
Size: 763 893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 942 976 986 1020 1656
Size: 942 34 10 34 636
Fragments arranged by size:
942 636 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 34 1656
Size: 34 1622
BfrBI ATG'CAT
Cuts at: 0 3 1558 1656
Size: 3 1555 98
Fragments arranged by size:
1555 98 3
BglI GCCn_nnn'nGGC
Cuts at: 0 157 1656
Size: 157 1499
BlpI GC'TnA_GC
Cuts at: 0 964 1656
Size: 964 692
Bme1580I G_kGCm'C
Cuts at: 0 1393 1656
Size: 1393 263
BmrI ACTGGGnnnn_n'
Cuts at: 0 610 1656
Size: 610 1046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 1656
Size: 79 1577
Bpu10I CC'TnA_GC
Cuts at: 0 763 1656
Size: 763 893
BsaI GGTCTCn'nnnn_
Cuts at: 0 1255 1656
Size: 1255 401
BsaAI yAC'GTr
Cuts at: 0 510 1656
Size: 510 1146
BsaBI GATnn'nnATC
Cuts at: 0 394 1656
Size: 394 1262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 1656
Size: 850 30 776
Fragments arranged by size:
850 776 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1210 1656
Size: 55 1155 446
Fragments arranged by size:
1155 446 55
BseYI C'CCAG_C
Cuts at: 0 654 1381 1656
Size: 654 727 275
Fragments arranged by size:
727 654 275
BsiHKAI G_wGCw'C
Cuts at: 0 203 600 1656
Size: 203 397 1056
Fragments arranged by size:
1056 397 203
Bsp1286I G_dGCh'C
Cuts at: 0 203 600 1393 1656
Size: 203 397 793 263
Fragments arranged by size:
793 397 263 203
BsrDI GCAATG_nn'
Cuts at: 0 1103 1169 1656
Size: 1103 66 487
Fragments arranged by size:
1103 487 66
BsrGI T'GTAC_A
Cuts at: 0 581 621 857 1088 1656
Size: 581 40 236 231 568
Fragments arranged by size:
581 568 236 231 40
BstYI r'GATC_y
Cuts at: 0 282 693 752 1656
Size: 282 411 59 904
Fragments arranged by size:
904 411 282 59
BtgI C'CryG_G
Cuts at: 0 698 1656
Size: 698 958
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 1656
Size: 249 1407
BtsI GCAGTG_nn'
Cuts at: 0 774 1656
Size: 774 882
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 33 68 1590 1625 1656
Size: 33 35 1522 35 31
Fragments arranged by size:
1522 35 35 33 31
DraI TTT'AAA
Cuts at: 0 734 1656
Size: 734 922
DrdI GACnn_nn'nnGTC
Cuts at: 0 483 1656
Size: 483 1173
EarI CTCTTCn'nnn_
Cuts at: 0 1195 1228 1656
Size: 1195 33 428
Fragments arranged by size:
1195 428 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1632 1656
Size: 1632 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 576 1656
Size: 384 192 1080
Fragments arranged by size:
1080 384 192
EcoO109I rG'GnC_Cy
Cuts at: 0 466 803 1656
Size: 466 337 853
Fragments arranged by size:
853 466 337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 397 468 1089 1656
Size: 397 71 621 567
Fragments arranged by size:
621 567 397 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 643 675 1274 1306 1656
Size: 352 32 259 32 599 32 350
Fragments arranged by size:
599 352 350 259 32 32 32
HincII GTy'rAC
Cuts at: 0 1232 1656
Size: 1232 424
HpaI GTT'AAC
Cuts at: 0 1232 1656
Size: 1232 424
MfeI C'AATT_G
Cuts at: 0 517 1361 1656
Size: 517 844 295
Fragments arranged by size:
844 517 295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 684 1039 1521 1656
Size: 554 130 355 482 135
Fragments arranged by size:
554 482 355 135 130
MspA1I CmG'CkG
Cuts at: 0 82 1274 1656
Size: 82 1192 382
Fragments arranged by size:
1192 382 82
NcoI C'CATG_G
Cuts at: 0 698 1656
Size: 698 958
NsiI A_TGCA'T
Cuts at: 0 5 1560 1656
Size: 5 1555 96
Fragments arranged by size:
1555 96 5
NspI r_CATG'y
Cuts at: 0 1332 1562 1656
Size: 1332 230 94
Fragments arranged by size:
1332 230 94
PasI CC'CwG_GG
Cuts at: 0 334 1656
Size: 334 1322
PciI A'CATG_T
Cuts at: 0 1328 1656
Size: 1328 328
PfoI T'CCnGG_A
Cuts at: 0 748 1656
Size: 748 908
PpuMI rG'GwC_Cy
Cuts at: 0 466 1656
Size: 466 1190
PsiI TTA'TAA
Cuts at: 0 1490 1656
Size: 1490 166
PvuII CAG'CTG
Cuts at: 0 82 1274 1656
Size: 82 1192 382
Fragments arranged by size:
1192 382 82
SanDI GG'GwC_CC
Cuts at: 0 466 1656
Size: 466 1190
SapI GCTCTTCn'nnn_
Cuts at: 0 1195 1656
Size: 1195 461
SfcI C'TryA_G
Cuts at: 0 1022 1656
Size: 1022 634
SmlI C'TyrA_G
Cuts at: 0 294 1656
Size: 294 1362
SphI G_CATG'C
Cuts at: 0 1562 1656
Size: 1562 94
StyI C'CwwG_G
Cuts at: 0 69 698 1656
Size: 69 629 958
Fragments arranged by size:
958 629 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 812 1373 1456 1656
Size: 812 561 83 200
Fragments arranged by size:
812 561 200 83
TatI w'GTAC_w
Cuts at: 0 581 621 857 1088 1656
Size: 581 40 236 231 568
Fragments arranged by size:
581 568 236 231 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 1656
Size: 596 32 1028
Fragments arranged by size:
1028 596 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 928 1445 1656
Size: 928 517 211
Fragments arranged by size:
928 517 211
XmnI GAAnn'nnTTC
Cuts at: 0 451 1656
Size: 451 1205
Enzymes that do cut:
AclI AcuI AflII AflIII ApoI BaeI BanI BbsI
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI
BplI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BsrDI BsrGI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EarI EciI Eco57MI EcoO109I FalI
Hin4I HincII HpaI MfeI MmeI MspA1I NcoI NsiI
NspI PasI PciI PfoI PpuMI PsiI PvuII SanDI
SapI SfcI SmlI SphI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanII BbeI BclI BglII BmgBI
BmtI BpmI BpuEI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EaeI EagI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII KasI KpnI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI