Restriction map for
pFN21AE5681
(Linear) (Six-base) MAPSORT of: KIEE5681.seq Check: 7335 from: 1 to: 1566
KIEE5681.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AccI GT'mk_AC
Cuts at: 0 695 1566
Size: 695 871
AclI AA'CG_TT
Cuts at: 0 1268 1566
Size: 1268 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 475 613 1218 1566
Size: 403 72 138 605 348
Fragments arranged by size:
605 403 348 138 72
AflIII A'CryG_T
Cuts at: 0 1460 1566
Size: 1460 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1007 1566
Size: 1007 559
AlwNI CAG_nnn'CTG
Cuts at: 0 369 548 1566
Size: 369 179 1018
Fragments arranged by size:
1018 369 179
ApoI r'AATT_y
Cuts at: 0 171 577 1566
Size: 171 406 989
Fragments arranged by size:
989 406 171
AseI AT'TA_AT
Cuts at: 0 587 1566
Size: 587 979
AvaI C'yCGr_G
Cuts at: 0 72 136 1183 1566
Size: 72 64 1047 383
Fragments arranged by size:
1047 383 72 64
BamHI G'GATC_C
Cuts at: 0 481 1566
Size: 481 1085
BanI G'GyrC_C
Cuts at: 0 76 665 739 1488 1566
Size: 76 589 74 749 78
Fragments arranged by size:
749 589 78 76 74
BanII G_rGCy'C
Cuts at: 0 155 812 1566
Size: 155 657 754
Fragments arranged by size:
754 657 155
BbeI G_GCGC'C
Cuts at: 0 80 1566
Size: 80 1486
BbsI GAAGACnn'nnnn_
Cuts at: 0 601 840 1304 1566
Size: 601 239 464 262
Fragments arranged by size:
601 464 262 239
BbvCI CC'TCA_GC
Cuts at: 0 1360 1393 1566
Size: 1360 33 173
Fragments arranged by size:
1360 173 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 393 427 1566
Size: 393 34 1139
Fragments arranged by size:
1139 393 34
BglII A'GATC_T
Cuts at: 0 620 1566
Size: 620 946
BlpI GC'TnA_GC
Cuts at: 0 49 1566
Size: 49 1517
Bme1580I G_kGCm'C
Cuts at: 0 742 986 1491 1566
Size: 742 244 505 75
Fragments arranged by size:
742 505 244 75
BmgBI CAC'GTC
Cuts at: 0 828 857 987 1566
Size: 828 29 130 579
Fragments arranged by size:
828 579 130 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 558 696 1479 1494 1566
Size: 435 123 138 783 15 72
Fragments arranged by size:
783 435 138 123 72 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 826 1514 1566
Size: 141 685 688 52
Fragments arranged by size:
688 685 141 52
Bpu10I CC'TnA_GC
Cuts at: 0 1360 1393 1566
Size: 1360 33 173
Fragments arranged by size:
1360 173 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 1566
Size: 157 1409
BsaHI Gr'CG_yC
Cuts at: 0 77 698 767 1066 1566
Size: 77 621 69 299 500
Fragments arranged by size:
621 500 299 77 69
BsaWI w'CCGG_w
Cuts at: 0 179 1566
Size: 179 1387
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 755 785 794 824 1566
Size: 755 30 9 30 742
Fragments arranged by size:
755 742 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 802 1093 1566
Size: 802 291 473
Fragments arranged by size:
802 473 291
BseYI C'CCAG_C
Cuts at: 0 1014 1072 1451 1566
Size: 1014 58 379 115
Fragments arranged by size:
1014 379 115 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1566
Size: 808 758
BsiEI CG_ry'CG
Cuts at: 0 1412 1566
Size: 1412 154
BsiHKAI G_wGCw'C
Cuts at: 0 50 238 812 1566
Size: 50 188 574 754
Fragments arranged by size:
754 574 188 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 758 1566
Size: 758 808
Bsp1286I G_dGCh'C
Cuts at: 0 50 155 238 742 812 986 1491 1566
Size: 50 105 83 504 70 174 505 75
Fragments arranged by size:
505 504 174 105 83 75 70 50
BspHI T'CATG_A
Cuts at: 0 1478 1566
Size: 1478 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 635 830 1566
Size: 635 195 736
Fragments arranged by size:
736 635 195
BsrDI GCAATG_nn'
Cuts at: 0 288 1566
Size: 288 1278
BsrFI r'CCGG_y
Cuts at: 0 391 1124 1566
Size: 391 733 442
Fragments arranged by size:
733 442 391
BsrGI T'GTAC_A
Cuts at: 0 539 1566
Size: 539 1027
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 369 1566
Size: 39 330 1197
Fragments arranged by size:
1197 330 39
BstEII G'GTnAC_C
Cuts at: 0 130 1566
Size: 130 1436
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1047 1566
Size: 1047 519
BstYI r'GATC_y
Cuts at: 0 481 620 1566
Size: 481 139 946
Fragments arranged by size:
946 481 139
BtgI C'CryG_G
Cuts at: 0 56 1155 1566
Size: 56 1099 411
Fragments arranged by size:
1099 411 56
BtsI GCAGTG_nn'
Cuts at: 0 253 989 1566
Size: 253 736 577
Fragments arranged by size:
736 577 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 826 1566
Size: 826 740
EaeI y'GGCC_r
Cuts at: 0 976 1175 1566
Size: 976 199 391
Fragments arranged by size:
976 391 199
EarI CTCTTCn'nnn_
Cuts at: 0 1039 1566
Size: 1039 527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 873 1566
Size: 873 693
EcoICRI GAG'CTC
Cuts at: 0 810 1566
Size: 810 756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 403 475 613 826 1218 1514 1566
Size: 141 262 72 138 213 392 296 52
Fragments arranged by size:
392 296 262 213 141 138 72 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1117 1566
Size: 1117 449
EcoO109I rG'GnC_Cy
Cuts at: 0 68 437 1338 1389 1566
Size: 68 369 901 51 177
Fragments arranged by size:
901 369 177 68 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 1218 1566
Size: 217 1001 348
Fragments arranged by size:
1001 348 217
HaeII r_GCGC'y
Cuts at: 0 56 80 872 1376 1566
Size: 56 24 792 504 190
Fragments arranged by size:
792 504 190 56 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 755 787 1566
Size: 755 32 779
Fragments arranged by size:
779 755 32
HincII GTy'rAC
Cuts at: 0 696 1566
Size: 696 870
KasI G'GCGC_C
Cuts at: 0 76 1566
Size: 76 1490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1566
Size: 839 727
MscI TGG'CCA
Cuts at: 0 978 1566
Size: 978 588
MslI CAynn'nnrTG
Cuts at: 0 535 1168 1566
Size: 535 633 398
Fragments arranged by size:
633 535 398
MspA1I CmG'CkG
Cuts at: 0 36 58 1014 1566
Size: 36 22 956 552
Fragments arranged by size:
956 552 36 22
NaeI GCC'GGC
Cuts at: 0 393 1126 1566
Size: 393 733 440
Fragments arranged by size:
733 440 393
NarI GG'CG_CC
Cuts at: 0 77 1566
Size: 77 1489
NgoMIV G'CCGG_C
Cuts at: 0 391 1124 1566
Size: 391 733 442
Fragments arranged by size:
733 442 391
PasI CC'CwG_GG
Cuts at: 0 1258 1342 1566
Size: 1258 84 224
Fragments arranged by size:
1258 224 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 1014 1223 1566
Size: 1014 209 343
Fragments arranged by size:
1014 343 209
PfoI T'CCnGG_A
Cuts at: 0 773 1566
Size: 773 793
PpuMI rG'GwC_Cy
Cuts at: 0 1338 1566
Size: 1338 228
PstI C_TGCA'G
Cuts at: 0 192 632 996 1121 1566
Size: 192 440 364 125 445
Fragments arranged by size:
445 440 364 192 125
PvuII CAG'CTG
Cuts at: 0 36 1014 1566
Size: 36 978 552
Fragments arranged by size:
978 552 36
SacI G_AGCT'C
Cuts at: 0 812 1566
Size: 812 754
SacII CC_GC'GG
Cuts at: 0 59 1566
Size: 59 1507
SalI G'TCGA_C
Cuts at: 0 694 1566
Size: 694 872
SanDI GG'GwC_CC
Cuts at: 0 1338 1566
Size: 1338 228
SbfI CC_TGCA'GG
Cuts at: 0 632 1121 1566
Size: 632 489 445
Fragments arranged by size:
632 489 445
SfcI C'TryA_G
Cuts at: 0 188 628 992 1117 1566
Size: 188 440 364 125 449
Fragments arranged by size:
449 440 364 188 125
SfoI GGC'GCC
Cuts at: 0 78 1566
Size: 78 1488
StuI AGG'CCT
Cuts at: 0 1140 1432 1566
Size: 1140 292 134
Fragments arranged by size:
1140 292 134
StyI C'CwwG_G
Cuts at: 0 1433 1566
Size: 1433 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 1398 1566
Size: 631 767 168
Fragments arranged by size:
767 631 168
TatI w'GTAC_w
Cuts at: 0 539 1566
Size: 539 1027
Tth111I GACn'n_nGTC
Cuts at: 0 769 1566
Size: 769 797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 1566
Size: 732 834
XmnI GAAnn'nnTTC
Cuts at: 0 578 1538 1566
Size: 578 960 28
Fragments arranged by size:
960 578 28
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PflMI PfoI PpuMI PstI PvuII SacI
SacII SalI SanDI SbfI SfcI SfoI StuI StyI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BciVI
BclI BfrBI BglI BmtI BplI BpuEI BsaAI BsaBI
BsiWI BsmI BspEI BsrBI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SapI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XhoI XmaI ZraI