Restriction map for
pFN21AE5679
(Linear) (Six-base) MAPSORT of: KIEE5679.seq Check: 6633 from: 1 to: 1509
KIEE5679.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1363 1509
Size: 799 564 146
Fragments arranged by size:
799 564 146
AflIII A'CryG_T
Cuts at: 0 519 1509
Size: 519 990
AlwNI CAG_nnn'CTG
Cuts at: 0 1231 1322 1509
Size: 1231 91 187
Fragments arranged by size:
1231 187 91
ApaI G_GGCC'C
Cuts at: 0 122 1509
Size: 122 1387
ApoI r'AATT_y
Cuts at: 0 110 837 1509
Size: 110 727 672
Fragments arranged by size:
727 672 110
BanI G'GyrC_C
Cuts at: 0 1026 1509
Size: 1026 483
BanII G_rGCy'C
Cuts at: 0 122 884 1509
Size: 122 762 625
Fragments arranged by size:
762 625 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1509
Size: 294 1215
BbvCI CC'TCA_GC
Cuts at: 0 362 1509
Size: 362 1147
BclI T'GATC_A
Cuts at: 0 266 548 1509
Size: 266 282 961
Fragments arranged by size:
961 282 266
BglI GCCn_nnn'nGGC
Cuts at: 0 324 1509
Size: 324 1185
Bme1580I G_kGCm'C
Cuts at: 0 122 1509
Size: 122 1387
BmgBI CAC'GTC
Cuts at: 0 522 1509
Size: 522 987
BmrI ACTGGGnnnn_n'
Cuts at: 0 444 1096 1509
Size: 444 652 413
Fragments arranged by size:
652 444 413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1358 1509
Size: 1358 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1509
Size: 898 611
Bpu10I CC'TnA_GC
Cuts at: 0 362 1509
Size: 362 1147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1138 1509
Size: 409 729 371
Fragments arranged by size:
729 409 371
BsaI GGTCTCn'nnnn_
Cuts at: 0 847 1486 1509
Size: 847 639 23
Fragments arranged by size:
847 639 23
BsaWI w'CCGG_w
Cuts at: 0 311 1509
Size: 311 1198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1509
Size: 52 1457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1509
Size: 1012 497
BsiHKAI G_wGCw'C
Cuts at: 0 884 1509
Size: 884 625
Bsp1286I G_dGCh'C
Cuts at: 0 122 884 1509
Size: 122 762 625
Fragments arranged by size:
762 625 122
BspHI T'CATG_A
Cuts at: 0 1206 1509
Size: 1206 303
BsrDI GCAATG_nn'
Cuts at: 0 391 443 1509
Size: 391 52 1066
Fragments arranged by size:
1066 391 52
BssSI C'ACGA_G
Cuts at: 0 927 1345 1509
Size: 927 418 164
Fragments arranged by size:
927 418 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 990 1509
Size: 990 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1173 1220 1509
Size: 1173 47 289
Fragments arranged by size:
1173 289 47
BstYI r'GATC_y
Cuts at: 0 3 255 648 1281 1415 1509
Size: 3 252 393 633 134 94
Fragments arranged by size:
633 393 252 134 94 3
BtgI C'CryG_G
Cuts at: 0 23 1509
Size: 23 1486
BtsI GCAGTG_nn'
Cuts at: 0 939 1509
Size: 939 570
DraIII CAC_nnn'GTG
Cuts at: 0 804 1509
Size: 804 705
EaeI y'GGCC_r
Cuts at: 0 20 962 1509
Size: 20 942 547
Fragments arranged by size:
942 547 20
EarI CTCTTCn'nnn_
Cuts at: 0 373 1509
Size: 373 1136
EcoICRI GAG'CTC
Cuts at: 0 882 1509
Size: 882 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 898 1363 1509
Size: 799 99 465 146
Fragments arranged by size:
799 465 146 99
EcoO109I rG'GnC_Cy
Cuts at: 0 118 530 1509
Size: 118 412 979
Fragments arranged by size:
979 412 118
EcoRI G'AATT_C
Cuts at: 0 110 837 1509
Size: 110 727 672
Fragments arranged by size:
727 672 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 560 592 816 848 1509
Size: 560 32 224 32 661
Fragments arranged by size:
661 560 224 32 32
HincII GTy'rAC
Cuts at: 0 232 1509
Size: 232 1277
HindIII A'AGCT_T
Cuts at: 0 103 625 1509
Size: 103 522 884
Fragments arranged by size:
884 522 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1037 1509
Size: 427 610 472
Fragments arranged by size:
610 472 427
MscI TGG'CCA
Cuts at: 0 964 1509
Size: 964 545
MslI CAynn'nnrTG
Cuts at: 0 544 974 1075 1330 1509
Size: 544 430 101 255 179
Fragments arranged by size:
544 430 255 179 101
MspA1I CmG'CkG
Cuts at: 0 416 819 1509
Size: 416 403 690
Fragments arranged by size:
690 416 403
PsiI TTA'TAA
Cuts at: 0 194 736 1509
Size: 194 542 773
Fragments arranged by size:
773 542 194
PspOMI G'GGCC_C
Cuts at: 0 118 1509
Size: 118 1391
PvuII CAG'CTG
Cuts at: 0 819 1509
Size: 819 690
SacI G_AGCT'C
Cuts at: 0 884 1509
Size: 884 625
ScaI AGT'ACT
Cuts at: 0 1219 1509
Size: 1219 290
SfcI C'TryA_G
Cuts at: 0 356 534 1051 1509
Size: 356 178 517 458
Fragments arranged by size:
517 458 356 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 324 1509
Size: 324 1185
SmlI C'TyrA_G
Cuts at: 0 424 1117 1509
Size: 424 693 392
Fragments arranged by size:
693 424 392
StuI AGG'CCT
Cuts at: 0 318 1509
Size: 318 1191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1509
Size: 1047 462
TatI w'GTAC_w
Cuts at: 0 1217 1509
Size: 1217 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 1509
Size: 705 32 772
Fragments arranged by size:
772 705 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1173 1509
Size: 1173 336
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI BanI BanII BbsI
BbvCI BclI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BseRI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
HincII HindIII MmeI MscI MslI MspA1I PsiI PspOMI
PvuII SacI ScaI SfcI SfiI SmlI StuI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BglII BlpI BmtI BsaAI BsaBI BsaHI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI