Restriction map for
pFN21AE5678
(Linear) (Six-base) MAPSORT of: KIEE5678.seq Check: 6331 from: 1 to: 1413
KIEE5678.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1365 1413
Size: 1365 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1293 1413
Size: 874 419 120
Fragments arranged by size:
874 419 120
AhdI GACnn_n'nnGTC
Cuts at: 0 672 1413
Size: 672 741
AlwNI CAG_nnn'CTG
Cuts at: 0 81 582 1032 1413
Size: 81 501 450 381
Fragments arranged by size:
501 450 381 81
ApaI G_GGCC'C
Cuts at: 0 288 1413
Size: 288 1125
ApoI r'AATT_y
Cuts at: 0 152 1413
Size: 152 1261
AvrII C'CTAG_G
Cuts at: 0 280 678 1413
Size: 280 398 735
Fragments arranged by size:
735 398 280
BanI G'GyrC_C
Cuts at: 0 1099 1413
Size: 1099 314
BanII G_rGCy'C
Cuts at: 0 288 1413
Size: 288 1125
BciVI GTATCCnnnnn_n'
Cuts at: 0 703 1413
Size: 703 710
BglII A'GATC_T
Cuts at: 0 815 1413
Size: 815 598
BlpI GC'TnA_GC
Cuts at: 0 950 1413
Size: 950 463
Bme1580I G_kGCm'C
Cuts at: 0 288 1413
Size: 288 1125
BmgBI CAC'GTC
Cuts at: 0 1397 1413
Size: 1397 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 484 543 1413
Size: 484 59 870
Fragments arranged by size:
870 484 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1235 1413
Size: 604 631 178
Fragments arranged by size:
631 604 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 430 1413
Size: 116 314 983
Fragments arranged by size:
983 314 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 1413
Size: 434 979
BsaHI Gr'CG_yC
Cuts at: 0 640 1413
Size: 640 773
BsaWI w'CCGG_w
Cuts at: 0 864 1413
Size: 864 549
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 883 1413
Size: 22 861 530
Fragments arranged by size:
861 530 22
BseYI C'CCAG_C
Cuts at: 0 81 1413
Size: 81 1332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1039 1413
Size: 178 861 374
Fragments arranged by size:
861 374 178
BsmI GAATG_Cn'
Cuts at: 0 1282 1413
Size: 1282 131
Bsp1286I G_dGCh'C
Cuts at: 0 288 1413
Size: 288 1125
BspHI T'CATG_A
Cuts at: 0 773 1413
Size: 773 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1365 1413
Size: 1365 48
BsrDI GCAATG_nn'
Cuts at: 0 428 1413
Size: 428 985
BsrFI r'CCGG_y
Cuts at: 0 350 1413
Size: 350 1063
BstAPI GCAn_nnn'nTGC
Cuts at: 0 976 1413
Size: 976 437
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1141 1413
Size: 1141 272
BstYI r'GATC_y
Cuts at: 0 815 1029 1413
Size: 815 214 384
Fragments arranged by size:
815 384 214
BtsI GCAGTG_nn'
Cuts at: 0 977 1413
Size: 977 436
DraIII CAC_nnn'GTG
Cuts at: 0 1062 1413
Size: 1062 351
EaeI y'GGCC_r
Cuts at: 0 827 1010 1413
Size: 827 183 403
Fragments arranged by size:
827 403 183
EarI CTCTTCn'nnn_
Cuts at: 0 797 1078 1379 1413
Size: 797 281 301 34
Fragments arranged by size:
797 301 281 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 874 1235 1293 1413
Size: 604 270 361 58 120
Fragments arranged by size:
604 361 270 120 58
EcoO109I rG'GnC_Cy
Cuts at: 0 284 924 1413
Size: 284 640 489
Fragments arranged by size:
640 489 284
FspI TGC'GCA
Cuts at: 0 434 1413
Size: 434 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 866 898 1413
Size: 658 32 176 32 515
Fragments arranged by size:
658 515 176 32 32
MfeI C'AATT_G
Cuts at: 0 66 1413
Size: 66 1347
MscI TGG'CCA
Cuts at: 0 829 1012 1413
Size: 829 183 401
Fragments arranged by size:
829 401 183
MslI CAynn'nnrTG
Cuts at: 0 1133 1139 1413
Size: 1133 6 274
Fragments arranged by size:
1133 274 6
MspA1I CmG'CkG
Cuts at: 0 954 1313 1413
Size: 954 359 100
Fragments arranged by size:
954 359 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 582 1413
Size: 582 831
PfoI T'CCnGG_A
Cuts at: 0 1025 1413
Size: 1025 388
PspOMI G'GGCC_C
Cuts at: 0 284 1413
Size: 284 1129
PstI C_TGCA'G
Cuts at: 0 1304 1413
Size: 1304 109
PvuII CAG'CTG
Cuts at: 0 954 1313 1413
Size: 954 359 100
Fragments arranged by size:
954 359 100
SapI GCTCTTCn'nnn_
Cuts at: 0 1379 1413
Size: 1379 34
SfcI C'TryA_G
Cuts at: 0 1300 1413
Size: 1300 113
SmlI C'TyrA_G
Cuts at: 0 131 445 1413
Size: 131 314 968
Fragments arranged by size:
968 314 131
StyI C'CwwG_G
Cuts at: 0 280 528 678 1166 1413
Size: 280 248 150 488 247
Fragments arranged by size:
488 280 248 247 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 230 1413
Size: 230 1183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1308 1340 1413
Size: 1308 32 73
Fragments arranged by size:
1308 73 32
Tth111I GACn'n_nGTC
Cuts at: 0 1117 1413
Size: 1117 296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 803 924 1164 1413
Size: 803 121 240 249
Fragments arranged by size:
803 249 240 121
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApoI AvrII BanI
BanII BciVI BglII BlpI Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI
BtsI DraIII EaeI EarI Eco57MI EcoO109I FspI Hin4I
MfeI MscI MslI MspA1I PflMI PfoI PspOMI PstI
PvuII SapI SfcI SmlI StyI TaqII TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BmtI BplI Bpu10I BsaAI BsaBI BsaXI BsiEI
BsiHKAI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XhoI
XmaI XmnI ZraI