Restriction map for
pFN21AE5670
(Linear) (Six-base) MAPSORT of: KIEE5670.seq Check: 9570 from: 1 to: 1599
KIEE5670.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1427 1599
Size: 1427 172
Acc65I G'GTAC_C
Cuts at: 0 1448 1599
Size: 1448 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1280 1599
Size: 697 583 319
Fragments arranged by size:
697 583 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 1599
Size: 543 1056
AlwNI CAG_nnn'CTG
Cuts at: 0 116 147 489 1599
Size: 116 31 342 1110
Fragments arranged by size:
1110 342 116 31
ApaI G_GGCC'C
Cuts at: 0 64 1599
Size: 64 1535
ApoI r'AATT_y
Cuts at: 0 538 706 865 938 1599
Size: 538 168 159 73 661
Fragments arranged by size:
661 538 168 159 73
AscI GG'CGCG_CC
Cuts at: 0 1409 1599
Size: 1409 190
AseI AT'TA_AT
Cuts at: 0 881 1599
Size: 881 718
AvaI C'yCGr_G
Cuts at: 0 283 1599
Size: 283 1316
AvrII C'CTAG_G
Cuts at: 0 793 1599
Size: 793 806
BanI G'GyrC_C
Cuts at: 0 1448 1599
Size: 1448 151
BanII G_rGCy'C
Cuts at: 0 64 407 748 1599
Size: 64 343 341 851
Fragments arranged by size:
851 343 341 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 235 1599
Size: 235 1364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 1599
Size: 456 34 1109
Fragments arranged by size:
1109 456 34
BclI T'GATC_A
Cuts at: 0 326 1185 1599
Size: 326 859 414
Fragments arranged by size:
859 414 326
BglI GCCn_nnn'nGGC
Cuts at: 0 418 1599
Size: 418 1181
BlpI GC'TnA_GC
Cuts at: 0 121 1599
Size: 121 1478
Bme1580I G_kGCm'C
Cuts at: 0 64 559 581 1599
Size: 64 495 22 1018
Fragments arranged by size:
1018 495 64 22
BmgBI CAC'GTC
Cuts at: 0 672 1470 1599
Size: 672 798 129
Fragments arranged by size:
798 672 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 94 477 546 1599
Size: 58 36 383 69 1053
Fragments arranged by size:
1053 383 69 58 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1247 1314 1599
Size: 911 336 67 285
Fragments arranged by size:
911 336 285 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 936 1599
Size: 936 663
BsaAI yAC'GTr
Cuts at: 0 576 1599
Size: 576 1023
BsaHI Gr'CG_yC
Cuts at: 0 24 188 1599
Size: 24 164 1411
Fragments arranged by size:
1411 164 24
BsaWI w'CCGG_w
Cuts at: 0 1541 1599
Size: 1541 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 1214 1244 1599
Size: 553 30 631 30 355
Fragments arranged by size:
631 553 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 582 1599
Size: 582 1017
BseYI C'CCAG_C
Cuts at: 0 57 79 1599
Size: 57 22 1520
Fragments arranged by size:
1520 57 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1599
Size: 376 1223
BsiHKAI G_wGCw'C
Cuts at: 0 407 748 1599
Size: 407 341 851
Fragments arranged by size:
851 407 341
BsmBI CGTCTCn'nnnn_
Cuts at: 0 200 1599
Size: 200 1399
Bsp1286I G_dGCh'C
Cuts at: 0 64 407 559 581 748 1599
Size: 64 343 152 22 167 851
Fragments arranged by size:
851 343 167 152 64 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1427 1599
Size: 1427 172
BsrDI GCAATG_nn'
Cuts at: 0 641 1155 1599
Size: 641 514 444
Fragments arranged by size:
641 514 444
BsrGI T'GTAC_A
Cuts at: 0 197 1599
Size: 197 1402
BssHII G'CGCG_C
Cuts at: 0 1409 1599
Size: 1409 190
BstAPI GCAn_nnn'nTGC
Cuts at: 0 522 697 1599
Size: 522 175 902
Fragments arranged by size:
902 522 175
BstEII G'GTnAC_C
Cuts at: 0 1084 1599
Size: 1084 515
BtgI C'CryG_G
Cuts at: 0 1125 1160 1599
Size: 1125 35 439
Fragments arranged by size:
1125 439 35
BtsI GCAGTG_nn'
Cuts at: 0 520 1599
Size: 520 1079
DraI TTT'AAA
Cuts at: 0 1521 1599
Size: 1521 78
EaeI y'GGCC_r
Cuts at: 0 1370 1487 1599
Size: 1370 117 112
Fragments arranged by size:
1370 117 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 1599
Size: 35 1564
EcoICRI GAG'CTC
Cuts at: 0 405 746 1599
Size: 405 341 853
Fragments arranged by size:
853 405 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 911 1247 1280 1314 1599
Size: 697 214 336 33 34 285
Fragments arranged by size:
697 336 285 214 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1339 1599
Size: 1339 260
EcoO109I rG'GnC_Cy
Cuts at: 0 735 1599
Size: 735 864
EcoRI G'AATT_C
Cuts at: 0 706 1599
Size: 706 893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 914 946 1599
Size: 914 32 653
Fragments arranged by size:
914 653 32
KpnI G_GTAC'C
Cuts at: 0 1452 1599
Size: 1452 147
MfeI C'AATT_G
Cuts at: 0 962 1599
Size: 962 637
MslI CAynn'nnrTG
Cuts at: 0 525 1165 1599
Size: 525 640 434
Fragments arranged by size:
640 525 434
MspA1I CmG'CkG
Cuts at: 0 147 624 1362 1599
Size: 147 477 738 237
Fragments arranged by size:
738 477 237 147
NcoI C'CATG_G
Cuts at: 0 1125 1160 1599
Size: 1125 35 439
Fragments arranged by size:
1125 439 35
NdeI CA'TA_TG
Cuts at: 0 1144 1599
Size: 1144 455
NspI r_CATG'y
Cuts at: 0 129 520 639 1599
Size: 129 391 119 960
Fragments arranged by size:
960 391 129 119
PasI CC'CwG_GG
Cuts at: 0 15 1599
Size: 15 1584
PflMI CCAn_nnn'nTGG
Cuts at: 0 106 1464 1599
Size: 106 1358 135
Fragments arranged by size:
1358 135 106
PfoI T'CCnGG_A
Cuts at: 0 731 1599
Size: 731 868
PpuMI rG'GwC_Cy
Cuts at: 0 735 1599
Size: 735 864
PspOMI G'GGCC_C
Cuts at: 0 60 1599
Size: 60 1539
PstI C_TGCA'G
Cuts at: 0 152 320 623 1599
Size: 152 168 303 976
Fragments arranged by size:
976 303 168 152
PvuII CAG'CTG
Cuts at: 0 147 1599
Size: 147 1452
SacI G_AGCT'C
Cuts at: 0 407 748 1599
Size: 407 341 851
Fragments arranged by size:
851 407 341
SexAI A'CCwGG_T
Cuts at: 0 1444 1599
Size: 1444 155
SfcI C'TryA_G
Cuts at: 0 148 316 619 1599
Size: 148 168 303 980
Fragments arranged by size:
980 303 168 148
SphI G_CATG'C
Cuts at: 0 129 520 1599
Size: 129 391 1079
Fragments arranged by size:
1079 391 129
StuI AGG'CCT
Cuts at: 0 585 1599
Size: 585 1014
StyI C'CwwG_G
Cuts at: 0 793 1125 1160 1463 1599
Size: 793 332 35 303 136
Fragments arranged by size:
793 332 303 136 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1126 1371 1599
Size: 1126 245 228
Fragments arranged by size:
1126 245 228
TatI w'GTAC_w
Cuts at: 0 197 1599
Size: 197 1402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1214 1246 1599
Size: 1214 32 353
Fragments arranged by size:
1214 353 32
XmnI GAAnn'nnTTC
Cuts at: 0 710 1599
Size: 710 889
Enzymes that do cut:
AarI Acc65I AcuI AlfI AlwNI ApaI ApoI AscI
AseI AvaI AvrII BanI BanII BbsI BcgI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrGI BssHII BstAPI BstEII BtgI
BtsI DraI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI Hin4I KpnI MfeI MslI MspA1I NcoI NdeI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SphI StuI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AsiSI BaeI BamHI BbeI BbvCI
BciVI BfrBI BglII BmtI BplI Bpu10I BpuEI BsaBI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssSI
BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EarI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MluI MmeI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI