Restriction map for
pFN21AE5669
(Linear) (Six-base) MAPSORT of: KIEE5669.seq Check: 4305 from: 1 to: 1602
KIEE5669.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 532 1602
Size: 84 448 1070
Fragments arranged by size:
1070 448 84
AfeI AGC'GCT
Cuts at: 0 709 1602
Size: 709 893
AleI CACnn'nnGTG
Cuts at: 0 874 1602
Size: 874 728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 231 1602
Size: 231 1371
AlwNI CAG_nnn'CTG
Cuts at: 0 459 510 933 1047 1358 1602
Size: 459 51 423 114 311 244
Fragments arranged by size:
459 423 311 244 114 51
AvaI C'yCGr_G
Cuts at: 0 488 1335 1602
Size: 488 847 267
Fragments arranged by size:
847 488 267
BanII G_rGCy'C
Cuts at: 0 293 1602
Size: 293 1309
BbsI GAAGACnn'nnnn_
Cuts at: 0 1031 1602
Size: 1031 571
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1160 1602
Size: 283 877 442
Fragments arranged by size:
877 442 283
BglII A'GATC_T
Cuts at: 0 476 1602
Size: 476 1126
Bme1580I G_kGCm'C
Cuts at: 0 750 1004 1602
Size: 750 254 598
Fragments arranged by size:
750 598 254
BmgBI CAC'GTC
Cuts at: 0 66 1602
Size: 66 1536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1602
Size: 1141 461
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 1602
Size: 331 1271
BsaAI yAC'GTr
Cuts at: 0 621 1523 1602
Size: 621 902 79
Fragments arranged by size:
902 621 79
BsaWI w'CCGG_w
Cuts at: 0 418 1602
Size: 418 1184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1512 1542 1602
Size: 1512 30 60
Fragments arranged by size:
1512 60 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 1047 1602
Size: 751 296 555
Fragments arranged by size:
751 555 296
BseYI C'CCAG_C
Cuts at: 0 1531 1602
Size: 1531 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 256 1093 1244 1602
Size: 34 222 837 151 358
Fragments arranged by size:
837 358 222 151 34
BsiEI CG_ry'CG
Cuts at: 0 278 1602
Size: 278 1324
BsiHKAI G_wGCw'C
Cuts at: 0 1571 1602
Size: 1571 31
Bsp1286I G_dGCh'C
Cuts at: 0 293 750 1004 1571 1602
Size: 293 457 254 567 31
Fragments arranged by size:
567 457 293 254 31
BspEI T'CCGG_A
Cuts at: 0 418 1602
Size: 418 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 1037 1602
Size: 509 528 565
Fragments arranged by size:
565 528 509
BsrDI GCAATG_nn'
Cuts at: 0 683 1602
Size: 683 919
BsrFI r'CCGG_y
Cuts at: 0 218 1602
Size: 218 1384
BssHII G'CGCG_C
Cuts at: 0 10 948 1602
Size: 10 938 654
Fragments arranged by size:
938 654 10
BstEII G'GTnAC_C
Cuts at: 0 579 1602
Size: 579 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1238 1602
Size: 1238 364
BstYI r'GATC_y
Cuts at: 0 476 968 1602
Size: 476 492 634
Fragments arranged by size:
634 492 476
Bsu36I CC'TnA_GG
Cuts at: 0 1573 1602
Size: 1573 29
BtgI C'CryG_G
Cuts at: 0 278 584 1088 1602
Size: 278 306 504 514
Fragments arranged by size:
514 504 306 278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 9 1602
Size: 9 1593
BtsI GCAGTG_nn'
Cuts at: 0 213 806 1013 1377 1602
Size: 213 593 207 364 225
Fragments arranged by size:
593 364 225 213 207
ClaI AT'CG_AT
Cuts at: 0 404 1602
Size: 404 1198
EaeI y'GGCC_r
Cuts at: 0 152 275 284 701 779 803 1193 1602
Size: 152 123 9 417 78 24 390 409
Fragments arranged by size:
417 409 390 152 123 78 24 9
EagI C'GGCC_G
Cuts at: 0 275 1602
Size: 275 1327
EarI CTCTTCn'nnn_
Cuts at: 0 1012 1186 1602
Size: 1012 174 416
Fragments arranged by size:
1012 416 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1505 1576 1602
Size: 1505 71 26
Fragments arranged by size:
1505 71 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 532 1141 1602
Size: 84 448 609 461
Fragments arranged by size:
609 461 448 84
EcoO109I rG'GnC_Cy
Cuts at: 0 1235 1602
Size: 1235 367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1048 1602
Size: 1048 554
HaeII r_GCGC'y
Cuts at: 0 711 1602
Size: 711 891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 1602
Size: 393 32 1177
Fragments arranged by size:
1177 393 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1445 1602
Size: 629 816 157
Fragments arranged by size:
816 629 157
MscI TGG'CCA
Cuts at: 0 703 805 1602
Size: 703 102 797
Fragments arranged by size:
797 703 102
MslI CAynn'nnrTG
Cuts at: 0 874 1602
Size: 874 728
MspA1I CmG'CkG
Cuts at: 0 360 784 1080 1602
Size: 360 424 296 522
Fragments arranged by size:
522 424 360 296
NaeI GCC'GGC
Cuts at: 0 220 1602
Size: 220 1382
NcoI C'CATG_G
Cuts at: 0 584 1088 1602
Size: 584 504 514
Fragments arranged by size:
584 514 504
NgoMIV G'CCGG_C
Cuts at: 0 218 1602
Size: 218 1384
NspI r_CATG'y
Cuts at: 0 212 1602
Size: 212 1390
PfoI T'CCnGG_A
Cuts at: 0 662 1602
Size: 662 940
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1462 1494 1602
Size: 1462 32 108
Fragments arranged by size:
1462 108 32
PstI C_TGCA'G
Cuts at: 0 83 506 992 1020 1363 1400 1602
Size: 83 423 486 28 343 37 202
Fragments arranged by size:
486 423 343 202 83 37 28
PvuII CAG'CTG
Cuts at: 0 360 1080 1602
Size: 360 720 522
Fragments arranged by size:
720 522 360
ScaI AGT'ACT
Cuts at: 0 628 1602
Size: 628 974
SexAI A'CCwGG_T
Cuts at: 0 197 668 1602
Size: 197 471 934
Fragments arranged by size:
934 471 197
SfcI C'TryA_G
Cuts at: 0 79 502 630 988 1016 1359 1365 1396
Size: 79 423 128 358 28 343 6 31
Cuts at: 1396 1602
Size: 206
Fragments arranged by size:
423 358 343 206 128 79 31 28
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 283 1602
Size: 283 1319
SmaI CCC'GGG
Cuts at: 0 490 1602
Size: 490 1112
SnaBI TAC'GTA
Cuts at: 0 1523 1602
Size: 1523 79
SphI G_CATG'C
Cuts at: 0 212 1602
Size: 212 1390
StyI C'CwwG_G
Cuts at: 0 371 584 1088 1173 1231 1602
Size: 371 213 504 85 58 371
Fragments arranged by size:
504 371 371 213 85 58
TatI w'GTAC_w
Cuts at: 0 365 626 1439 1602
Size: 365 261 813 163
Fragments arranged by size:
813 365 261 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1602
Size: 551 32 1019
Fragments arranged by size:
1019 551 32
Tth111I GACn'n_nGTC
Cuts at: 0 670 1602
Size: 670 932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 394 720 1602
Size: 394 326 882
Fragments arranged by size:
882 394 326
XmaI C'CCGG_G
Cuts at: 0 488 1602
Size: 488 1114
XmnI GAAnn'nnTTC
Cuts at: 0 1246 1602
Size: 1246 356
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI AvaI BanII BbsI
BglI BglII Bme1580I BmgBI BpmI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrDI BsrFI BssHII BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI EaeI EagI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PfoI PsrI PstI
PvuII ScaI SexAI SfcI SfiI SmaI SnaBI SphI
StyI TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsaHI BsiWI BsmI BsmBI BspHI BsrBI BsrGI
BssSI BstAPI BstBI BstZ17I CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SfoI SgfI SgrAI SmlI SpeI SrfI SspI StuI
SwaI TaqII XbaI XhoI ZraI