(Linear) (Six-base) MAPSORT of: KIEE5667.seq  Check: 9709  from: 1  to: 1569



KIEE5667.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    1202    1300    1569 
   Size:         70    1132      98     269

  Fragments arranged by size: 

                1132     269      98      70
 
AleI CACnn'nnGTG

Cuts at:      0     205    1569 
   Size:        205    1364
 
AlwNI CAG_nnn'CTG

Cuts at:      0     327    1569 
   Size:        327    1242
 
ApoI r'AATT_y

Cuts at:      0     757     916    1019    1270    1436    1471    1569 
   Size:        757     159     103     251     166      35      98

  Fragments arranged by size: 

                 757     251     166     159     103      98      35
 
AseI AT'TA_AT

Cuts at:      0     515    1166    1569 
   Size:        515     651     403

  Fragments arranged by size: 

                 651     515     403
 
AvaI C'yCGr_G

Cuts at:      0    1264    1569 
   Size:       1264     305
 
BanI G'GyrC_C

Cuts at:      0      20    1569 
   Size:         20    1549
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     494     876    1307    1569 
   Size:        494     382     431     262

  Fragments arranged by size: 

                 494     431     382     262
 
BbvCI CC'TCA_GC

Cuts at:      0       5    1569 
   Size:          5    1564
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     589    1569 
   Size:        589     980
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     309     573    1569 
   Size:        309     264     996

  Fragments arranged by size: 

                 996     309     264
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     791    1525    1569 
   Size:        791     734      44

  Fragments arranged by size: 

                 791     734      44
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    1569 
   Size:          5    1564
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1432    1448    1569 
   Size:       1432      16     121

  Fragments arranged by size: 

                1432     121      16
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1569 
   Size:         10    1559
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     802    1569 
   Size:        802     767
 
BsrGI T'GTAC_A

Cuts at:      0    1014    1569 
   Size:       1014     555
 
BstBI TT'CG_AA

Cuts at:      0      37    1569 
   Size:         37    1532
 
DraI TTT'AAA

Cuts at:      0     338    1569 
   Size:        338    1231
 
EarI CTCTTCn'nnn_

Cuts at:      0    1341    1569 
   Size:       1341     228
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     791    1202    1300    1525    1569 
   Size:         70     721     411      98     225      44

  Fragments arranged by size: 

                 721     411     225      98      70      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     277    1569 
   Size:        277    1292
 
EcoRI G'AATT_C

Cuts at:      0     757    1569 
   Size:        757     812
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     298    1426    1569 
   Size:        298    1128     143

  Fragments arranged by size: 

                1128     298     143
 
HincII GTy'rAC

Cuts at:      0     397    1569 
   Size:        397    1172
 
MslI CAynn'nnrTG

Cuts at:      0     205    1569 
   Size:        205    1364
 
MspA1I CmG'CkG

Cuts at:      0     327     665    1041    1322    1569 
   Size:        327     338     376     281     247

  Fragments arranged by size: 

                 376     338     327     281     247
 
NdeI CA'TA_TG

Cuts at:      0     158    1569 
   Size:        158    1411
 
NspI r_CATG'y

Cuts at:      0     422    1088    1569 
   Size:        422     666     481

  Fragments arranged by size: 

                 666     481     422
 
PpuMI rG'GwC_Cy

Cuts at:      0     277    1569 
   Size:        277    1292
 
PvuII CAG'CTG

Cuts at:      0     327     665    1041    1569 
   Size:        327     338     376     528

  Fragments arranged by size: 

                 528     376     338     327
 
ScaI AGT'ACT

Cuts at:      0    1053    1569 
   Size:       1053     516
 
SmlI C'TyrA_G

Cuts at:      0    1264    1411    1463    1569 
   Size:       1264     147      52     106

  Fragments arranged by size: 

                1264     147     106      52
 
SphI G_CATG'C

Cuts at:      0    1088    1569 
   Size:       1088     481
 
SspI AAT'ATT

Cuts at:      0    1063    1569 
   Size:       1063     506
 
StyI C'CwwG_G

Cuts at:      0     556    1569 
   Size:        556    1013
 
TatI w'GTAC_w

Cuts at:      0     296    1014    1051    1569 
   Size:        296     718      37     518

  Fragments arranged by size: 

                 718     518     296      37
 
XhoI C'TCGA_G

Cuts at:      0    1264    1569 
   Size:       1264     305
 
XmnI GAAnn'nnTTC

Cuts at:      0    1150    1472    1557    1569 
   Size:       1150     322      85      12

  Fragments arranged by size: 

                1150     322      85      12
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     AseI     AvaI     BanI     BbsI
    BbvCI    BciVI     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BseRI
    BsrGI    BstBI     DraI     EarI  Eco57MI EcoO109I    EcoRI     FalI
   HincII     MslI   MspA1I     NdeI     NspI    PpuMI    PvuII     ScaI
     SmlI     SphI     SspI     StyI     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XmaI     ZraI