Restriction map for
pFN21AE5659
(Linear) (Six-base) MAPSORT of: KIEE5659.seq Check: 8943 from: 1 to: 1479
KIEE5659.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 63 1479
Size: 63 1416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1479
Size: 740 739
AfeI AGC'GCT
Cuts at: 0 187 266 844 1479
Size: 187 79 578 635
Fragments arranged by size:
635 578 187 79
AleI CACnn'nnGTG
Cuts at: 0 1087 1479
Size: 1087 392
AlwNI CAG_nnn'CTG
Cuts at: 0 86 1479
Size: 86 1393
ApaLI G'TGCA_C
Cuts at: 0 76 1479
Size: 76 1403
ApoI r'AATT_y
Cuts at: 0 1204 1479
Size: 1204 275
AvaI C'yCGr_G
Cuts at: 0 291 785 1118 1479
Size: 291 494 333 361
Fragments arranged by size:
494 361 333 291
BamHI G'GATC_C
Cuts at: 0 437 1479
Size: 437 1042
BanII G_rGCy'C
Cuts at: 0 220 935 1479
Size: 220 715 544
Fragments arranged by size:
715 544 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 484 1479
Size: 484 995
BbvCI CC'TCA_GC
Cuts at: 0 1411 1479
Size: 1411 68
BciVI GTATCCnnnnn_n'
Cuts at: 0 1132 1450 1479
Size: 1132 318 29
Fragments arranged by size:
1132 318 29
Bme1580I G_kGCm'C
Cuts at: 0 80 1094 1159 1479
Size: 80 1014 65 320
Fragments arranged by size:
1014 320 80 65
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 1159 1479
Size: 322 837 320
Fragments arranged by size:
837 322 320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1334 1479
Size: 628 706 145
Fragments arranged by size:
706 628 145
Bpu10I CC'TnA_GC
Cuts at: 0 1411 1479
Size: 1411 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 898 1479
Size: 898 581
BsaAI yAC'GTr
Cuts at: 0 213 1479
Size: 213 1266
BsaWI w'CCGG_w
Cuts at: 0 398 1479
Size: 398 1081
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 1479
Size: 5 30 1444
Fragments arranged by size:
1444 30 5
BseYI C'CCAG_C
Cuts at: 0 182 664 1479
Size: 182 482 815
Fragments arranged by size:
815 482 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1479
Size: 857 622
BsiHKAI G_wGCw'C
Cuts at: 0 80 758 1479
Size: 80 678 721
Fragments arranged by size:
721 678 80
Bsp1286I G_dGCh'C
Cuts at: 0 80 220 758 935 1094 1159 1479
Size: 80 140 538 177 159 65 320
Fragments arranged by size:
538 320 177 159 140 80 65
BspEI T'CCGG_A
Cuts at: 0 398 1479
Size: 398 1081
BspHI T'CATG_A
Cuts at: 0 944 1479
Size: 944 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 63 366 1109 1479
Size: 63 303 743 370
Fragments arranged by size:
743 370 303 63
BsrBI CCG'CTC
Cuts at: 0 1475 1479
Size: 1475 4
BsrDI GCAATG_nn'
Cuts at: 0 432 1479
Size: 432 1047
BsrFI r'CCGG_y
Cuts at: 0 195 1479
Size: 195 1284
BsrGI T'GTAC_A
Cuts at: 0 848 1479
Size: 848 631
BssHII G'CGCG_C
Cuts at: 0 521 1479
Size: 521 958
BssSI C'ACGA_G
Cuts at: 0 808 1087 1479
Size: 808 279 392
Fragments arranged by size:
808 392 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1038 1479
Size: 1038 441
BstYI r'GATC_y
Cuts at: 0 437 1479
Size: 437 1042
BtgI C'CryG_G
Cuts at: 0 20 1479
Size: 20 1459
EaeI y'GGCC_r
Cuts at: 0 878 1479
Size: 878 601
EarI CTCTTCn'nnn_
Cuts at: 0 139 556 959 1479
Size: 139 417 403 520
Fragments arranged by size:
520 417 403 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 740 1334 1479
Size: 628 112 594 145
Fragments arranged by size:
628 594 145 112
EcoO109I rG'GnC_Cy
Cuts at: 0 103 789 1479
Size: 103 686 690
Fragments arranged by size:
690 686 103
EcoRV GAT'ATC
Cuts at: 0 1404 1479
Size: 1404 75
FspI TGC'GCA
Cuts at: 0 323 1479
Size: 323 1156
FspAI rTGC'GCAy
Cuts at: 0 323 1479
Size: 323 1156
HaeII r_GCGC'y
Cuts at: 0 189 268 846 1479
Size: 189 79 578 633
Fragments arranged by size:
633 578 189 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 721 753 917 949 1130 1162
Size: 274 32 415 32 164 32 181 32
Cuts at: 1162 1479
Size: 317
Fragments arranged by size:
415 317 274 181 164 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1392 1479
Size: 1392 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1121 1479
Size: 122 999 358
Fragments arranged by size:
999 358 122
MslI CAynn'nnrTG
Cuts at: 0 232 1087 1479
Size: 232 855 392
Fragments arranged by size:
855 392 232
MspA1I CmG'CkG
Cuts at: 0 86 1074 1479
Size: 86 988 405
Fragments arranged by size:
988 405 86
PpuMI rG'GwC_Cy
Cuts at: 0 789 1479
Size: 789 690
PvuII CAG'CTG
Cuts at: 0 86 1479
Size: 86 1393
SmaI CCC'GGG
Cuts at: 0 787 1479
Size: 787 692
SspI AAT'ATT
Cuts at: 0 156 1479
Size: 156 1323
StuI AGG'CCT
Cuts at: 0 1322 1479
Size: 1322 157
StyI C'CwwG_G
Cuts at: 0 483 1151 1479
Size: 483 668 328
Fragments arranged by size:
668 483 328
TatI w'GTAC_w
Cuts at: 0 848 1265 1479
Size: 848 417 214
Fragments arranged by size:
848 417 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 1289 1321 1479
Size: 5 32 1252 32 158
Fragments arranged by size:
1252 158 32 32 5
XmaI C'CCGG_G
Cuts at: 0 785 1479
Size: 785 694
XmnI GAAnn'nnTTC
Cuts at: 0 133 1479
Size: 133 1346
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaLI ApoI AvaI
BamHI BanII BbsI BbvCI BciVI Bme1580I BplI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstYI BtgI EaeI EarI Eco57MI
EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII MmeI
MslI MspA1I PpuMI PvuII SmaI SspI StuI StyI
TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaBI BsaHI BseRI BsiEI
BsiWI BsmI BsmBI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FalI FseI HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI