Restriction map for
pFN21AE5654
(Linear) (Six-base) MAPSORT of: KIEE5654.seq Check: 8416 from: 1 to: 1629
KIEE5654.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AatII G_ACGT'C
Cuts at: 0 113 1629
Size: 113 1516
AccI GT'mk_AC
Cuts at: 0 1298 1629
Size: 1298 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 868 1629
Size: 856 12 761
Fragments arranged by size:
856 761 12
AflII C'TTAA_G
Cuts at: 0 688 1629
Size: 688 941
AleI CACnn'nnGTG
Cuts at: 0 1249 1629
Size: 1249 380
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 1629
Size: 193 32 1404
Fragments arranged by size:
1404 193 32
BamHI G'GATC_C
Cuts at: 0 388 1629
Size: 388 1241
BanI G'GyrC_C
Cuts at: 0 79 286 1243 1629
Size: 79 207 957 386
Fragments arranged by size:
957 386 207 79
BanII G_rGCy'C
Cuts at: 0 160 1629
Size: 160 1469
BbeI G_GCGC'C
Cuts at: 0 83 1629
Size: 83 1546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1057 1091 1629
Size: 1057 34 538
Fragments arranged by size:
1057 538 34
BclI T'GATC_A
Cuts at: 0 1512 1629
Size: 1512 117
BglI GCCn_nnn'nGGC
Cuts at: 0 193 1629
Size: 193 1436
Bme1580I G_kGCm'C
Cuts at: 0 1246 1629
Size: 1246 383
BmrI ACTGGGnnnn_n'
Cuts at: 0 1573 1629
Size: 1573 56
Bpu10I CC'TnA_GC
Cuts at: 0 537 1629
Size: 537 1092
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 480 1629
Size: 344 136 1149
Fragments arranged by size:
1149 344 136
BsaHI Gr'CG_yC
Cuts at: 0 80 110 185 1629
Size: 80 30 75 1444
Fragments arranged by size:
1444 80 75 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 1629
Size: 400 30 1199
Fragments arranged by size:
1199 400 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 1629
Size: 424 1205
BseYI C'CCAG_C
Cuts at: 0 60 1629
Size: 60 1569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1629
Size: 130 1499
BsiHKAI G_wGCw'C
Cuts at: 0 997 1629
Size: 997 632
Bsp1286I G_dGCh'C
Cuts at: 0 160 997 1246 1629
Size: 160 837 249 383
Fragments arranged by size:
837 383 249 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 102 1629
Size: 102 1527
BsrDI GCAATG_nn'
Cuts at: 0 675 1305 1629
Size: 675 630 324
Fragments arranged by size:
675 630 324
BstEII G'GTnAC_C
Cuts at: 0 1394 1629
Size: 1394 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1486 1629
Size: 1486 143
BstYI r'GATC_y
Cuts at: 0 388 1629
Size: 388 1241
BstZ17I GTA'TAC
Cuts at: 0 1299 1629
Size: 1299 330
Bsu36I CC'TnA_GG
Cuts at: 0 274 1629
Size: 274 1355
BtgI C'CryG_G
Cuts at: 0 197 205 1629
Size: 197 8 1424
Fragments arranged by size:
1424 197 8
BtsI GCAGTG_nn'
Cuts at: 0 598 1629
Size: 598 1031
DraIII CAC_nnn'GTG
Cuts at: 0 153 1629
Size: 153 1476
EaeI y'GGCC_r
Cuts at: 0 194 248 1549 1629
Size: 194 54 1301 80
Fragments arranged by size:
1301 194 80 54
EarI CTCTTCn'nnn_
Cuts at: 0 332 639 832 1226 1629
Size: 332 307 193 394 403
Fragments arranged by size:
403 394 332 307 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1064 1629
Size: 1064 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 868 1629
Size: 856 12 761
Fragments arranged by size:
856 761 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1629
Size: 691 938
EcoO109I rG'GnC_Cy
Cuts at: 0 64 1450 1629
Size: 64 1386 179
Fragments arranged by size:
1386 179 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 786 1629
Size: 315 471 843
Fragments arranged by size:
843 471 315
FspI TGC'GCA
Cuts at: 0 98 1629
Size: 98 1531
HaeII r_GCGC'y
Cuts at: 0 83 1057 1629
Size: 83 974 572
Fragments arranged by size:
974 572 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 1629
Size: 616 32 981
Fragments arranged by size:
981 616 32
HincII GTy'rAC
Cuts at: 0 366 1629
Size: 366 1263
HindIII A'AGCT_T
Cuts at: 0 235 1288 1629
Size: 235 1053 341
Fragments arranged by size:
1053 341 235
KasI G'GCGC_C
Cuts at: 0 79 1629
Size: 79 1550
MfeI C'AATT_G
Cuts at: 0 445 742 1629
Size: 445 297 887
Fragments arranged by size:
887 445 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1091 1503 1629
Size: 404 687 412 126
Fragments arranged by size:
687 412 404 126
MscI TGG'CCA
Cuts at: 0 196 250 1629
Size: 196 54 1379
Fragments arranged by size:
1379 196 54
MslI CAynn'nnrTG
Cuts at: 0 1249 1629
Size: 1249 380
MspA1I CmG'CkG
Cuts at: 0 36 60 144 207 1081 1629
Size: 36 24 84 63 874 548
Fragments arranged by size:
874 548 84 63 36 24
NarI GG'CG_CC
Cuts at: 0 80 1629
Size: 80 1549
NdeI CA'TA_TG
Cuts at: 0 1041 1629
Size: 1041 588
PflMI CCAn_nnn'nTGG
Cuts at: 0 1179 1629
Size: 1179 450
PfoI T'CCnGG_A
Cuts at: 0 620 1629
Size: 620 1009
PpuMI rG'GwC_Cy
Cuts at: 0 64 1450 1629
Size: 64 1386 179
Fragments arranged by size:
1386 179 64
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 475 507 1629
Size: 475 32 1122
Fragments arranged by size:
1122 475 32
PvuII CAG'CTG
Cuts at: 0 1081 1629
Size: 1081 548
SacII CC_GC'GG
Cuts at: 0 208 1629
Size: 208 1421
SanDI GG'GwC_CC
Cuts at: 0 64 1629
Size: 64 1565
SapI GCTCTTCn'nnn_
Cuts at: 0 639 832 1629
Size: 639 193 797
Fragments arranged by size:
797 639 193
ScaI AGT'ACT
Cuts at: 0 1387 1629
Size: 1387 242
SexAI A'CCwGG_T
Cuts at: 0 149 1629
Size: 149 1480
SfcI C'TryA_G
Cuts at: 0 506 1629
Size: 506 1123
SfoI GGC'GCC
Cuts at: 0 81 1629
Size: 81 1548
SmlI C'TyrA_G
Cuts at: 0 359 495 688 1629
Size: 359 136 193 941
Fragments arranged by size:
941 359 193 136
StuI AGG'CCT
Cuts at: 0 1002 1629
Size: 1002 627
StyI C'CwwG_G
Cuts at: 0 245 638 1247 1327 1629
Size: 245 393 609 80 302
Fragments arranged by size:
609 393 302 245 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1629
Size: 148 1481
TatI w'GTAC_w
Cuts at: 0 1385 1629
Size: 1385 244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1308 1340 1629
Size: 1118 32 158 32 289
Fragments arranged by size:
1118 289 158 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1629
Size: 1254 375
XmnI GAAnn'nnTTC
Cuts at: 0 475 841 926 1629
Size: 475 366 85 703
Fragments arranged by size:
703 475 366 85
ZraI GAC'GTC
Cuts at: 0 111 1629
Size: 111 1518
Enzymes that do cut:
AatII AccI AcuI AflII AleI AloI BamHI BanI
BanII BbeI BcgI BclI BglI Bme1580I BmrI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MslI MspA1I NarI NdeI PflMI PfoI
PpuMI PsrI PvuII SacII SanDI SapI ScaI SexAI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbsI BbvCI BciVI BfrBI BglII BlpI
BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BtgZI ClaI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI
HpaI KpnI MluI NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacI SalI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI