Restriction map for
pFN21AE5652
(Linear) (Six-base) MAPSORT of: KIEE5652.seq Check: 3788 from: 1 to: 1515
KIEE5652.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1131 1515
Size: 307 824 384
Fragments arranged by size:
824 384 307
AflIII A'CryG_T
Cuts at: 0 164 1515
Size: 164 1351
AleI CACnn'nnGTG
Cuts at: 0 1453 1515
Size: 1453 62
AlwNI CAG_nnn'CTG
Cuts at: 0 100 1515
Size: 100 1415
ApaLI G'TGCA_C
Cuts at: 0 982 1515
Size: 982 533
BanI G'GyrC_C
Cuts at: 0 713 1120 1515
Size: 713 407 395
Fragments arranged by size:
713 407 395
BanII G_rGCy'C
Cuts at: 0 7 472 1130 1515
Size: 7 465 658 385
Fragments arranged by size:
658 465 385 7
BbeI G_GCGC'C
Cuts at: 0 717 1515
Size: 717 798
BbsI GAAGACnn'nnnn_
Cuts at: 0 777 960 1033 1515
Size: 777 183 73 482
Fragments arranged by size:
777 482 183 73
BciVI GTATCCnnnnn_n'
Cuts at: 0 440 1515
Size: 440 1075
BclI T'GATC_A
Cuts at: 0 866 1515
Size: 866 649
BglI GCCn_nnn'nGGC
Cuts at: 0 45 97 1515
Size: 45 52 1418
Fragments arranged by size:
1418 52 45
BglII A'GATC_T
Cuts at: 0 344 677 1515
Size: 344 333 838
Fragments arranged by size:
838 344 333
BlpI GC'TnA_GC
Cuts at: 0 101 1515
Size: 101 1414
Bme1580I G_kGCm'C
Cuts at: 0 986 1515
Size: 986 529
BmgBI CAC'GTC
Cuts at: 0 486 1515
Size: 486 1029
BmrI ACTGGGnnnn_n'
Cuts at: 0 169 440 1515
Size: 169 271 1075
Fragments arranged by size:
1075 271 169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 937 1515
Size: 937 578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 406 577 1515
Size: 244 162 171 938
Fragments arranged by size:
938 244 171 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 868 1515
Size: 238 630 647
Fragments arranged by size:
647 630 238
BsaBI GATnn'nnATC
Cuts at: 0 1231 1515
Size: 1231 284
BsaHI Gr'CG_yC
Cuts at: 0 714 1515
Size: 714 801
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 781 811 1515
Size: 781 30 704
Fragments arranged by size:
781 704 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 829 1515
Size: 688 141 686
Fragments arranged by size:
688 686 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1515
Size: 320 1195
BsiHKAI G_wGCw'C
Cuts at: 0 107 472 782 977 986 1130 1515
Size: 107 365 310 195 9 144 385
Fragments arranged by size:
385 365 310 195 144 107 9
BsmI GAATG_Cn'
Cuts at: 0 1460 1515
Size: 1460 55
Bsp1286I G_dGCh'C
Cuts at: 0 7 107 472 782 977 986 1130 1515
Size: 7 100 365 310 195 9 144 385
Fragments arranged by size:
385 365 310 195 144 100 9 7
BspHI T'CATG_A
Cuts at: 0 1064 1515
Size: 1064 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 800 1052 1515
Size: 800 252 463
Fragments arranged by size:
800 463 252
BsrBI CCG'CTC
Cuts at: 0 15 120 611 1515
Size: 15 105 491 904
Fragments arranged by size:
904 491 105 15
BsrGI T'GTAC_A
Cuts at: 0 704 1515
Size: 704 811
BssSI C'ACGA_G
Cuts at: 0 1257 1515
Size: 1257 258
BstEII G'GTnAC_C
Cuts at: 0 816 1515
Size: 816 699
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1515
Size: 894 621
BstYI r'GATC_y
Cuts at: 0 344 677 1515
Size: 344 333 838
Fragments arranged by size:
838 344 333
BtgI C'CryG_G
Cuts at: 0 43 1415 1515
Size: 43 1372 100
Fragments arranged by size:
1372 100 43
ClaI AT'CG_AT
Cuts at: 0 560 1515
Size: 560 955
EaeI y'GGCC_r
Cuts at: 0 83 455 1499 1515
Size: 83 372 1044 16
Fragments arranged by size:
1044 372 83 16
EarI CTCTTCn'nnn_
Cuts at: 0 585 1515
Size: 585 930
EcoICRI GAG'CTC
Cuts at: 0 470 1128 1515
Size: 470 658 387
Fragments arranged by size:
658 470 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1131 1515
Size: 307 824 384
Fragments arranged by size:
824 384 307
EcoO109I rG'GnC_Cy
Cuts at: 0 89 1109 1515
Size: 89 1020 406
Fragments arranged by size:
1020 406 89
HaeII r_GCGC'y
Cuts at: 0 34 717 1515
Size: 34 683 798
Fragments arranged by size:
798 683 34
KasI G'GCGC_C
Cuts at: 0 713 1515
Size: 713 802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1515
Size: 824 691
MscI TGG'CCA
Cuts at: 0 85 1501 1515
Size: 85 1416 14
Fragments arranged by size:
1416 85 14
MslI CAynn'nnrTG
Cuts at: 0 1063 1453 1515
Size: 1063 390 62
Fragments arranged by size:
1063 390 62
MspA1I CmG'CkG
Cuts at: 0 45 210 1515
Size: 45 165 1305
Fragments arranged by size:
1305 165 45
NarI GG'CG_CC
Cuts at: 0 714 1515
Size: 714 801
NcoI C'CATG_G
Cuts at: 0 1415 1515
Size: 1415 100
PasI CC'CwG_GG
Cuts at: 0 1052 1515
Size: 1052 463
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 254 1515
Size: 240 14 1261
Fragments arranged by size:
1261 240 14
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 910 942 1515
Size: 910 32 573
Fragments arranged by size:
910 573 32
PvuII CAG'CTG
Cuts at: 0 210 1515
Size: 210 1305
SacI G_AGCT'C
Cuts at: 0 472 1130 1515
Size: 472 658 385
Fragments arranged by size:
658 472 385
SacII CC_GC'GG
Cuts at: 0 46 1515
Size: 46 1469
SapI GCTCTTCn'nnn_
Cuts at: 0 585 1515
Size: 585 930
SfcI C'TryA_G
Cuts at: 0 155 987 1515
Size: 155 832 528
Fragments arranged by size:
832 528 155
SfoI GGC'GCC
Cuts at: 0 715 1515
Size: 715 800
SmlI C'TyrA_G
Cuts at: 0 259 421 592 1515
Size: 259 162 171 923
Fragments arranged by size:
923 259 171 162
StyI C'CwwG_G
Cuts at: 0 1415 1515
Size: 1415 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1011 1515
Size: 1011 504
TatI w'GTAC_w
Cuts at: 0 425 659 704 1515
Size: 425 234 45 811
Fragments arranged by size:
811 425 234 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1186 1218 1515
Size: 1186 32 297
Fragments arranged by size:
1186 297 32
Tth111I GACn'n_nGTC
Cuts at: 0 175 1515
Size: 175 1340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1515
Size: 207 1308
XmnI GAAnn'nnTTC
Cuts at: 0 1144 1515
Size: 1144 371
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI
BssSI BstEII BstXI BstYI BtgI ClaI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII KasI MmeI MscI MslI
MspA1I NarI NcoI PasI PflMI PsrI PvuII SacI
SacII SapI SfcI SfoI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BfrBI BmtI
BpmI Bpu10I BsaAI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BsrDI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI