Restriction map for
pFN21AE5651
(Linear) (Six-base) MAPSORT of: KIEE5651.seq Check: 9942 from: 1 to: 1569
KIEE5651.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 693 1569
Size: 693 876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1569
Size: 1021 548
AflIII A'CryG_T
Cuts at: 0 1407 1569
Size: 1407 162
AleI CACnn'nnGTG
Cuts at: 0 856 1569
Size: 856 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1364 1569
Size: 1364 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1423 1455 1569
Size: 1423 32 114
Fragments arranged by size:
1423 114 32
AlwNI CAG_nnn'CTG
Cuts at: 0 178 651 1569
Size: 178 473 918
Fragments arranged by size:
918 473 178
ApaI G_GGCC'C
Cuts at: 0 339 1569
Size: 339 1230
ApoI r'AATT_y
Cuts at: 0 1031 1569
Size: 1031 538
AvaI C'yCGr_G
Cuts at: 0 33 260 1280 1569
Size: 33 227 1020 289
Fragments arranged by size:
1020 289 227 33
BamHI G'GATC_C
Cuts at: 0 37 520 1569
Size: 37 483 1049
Fragments arranged by size:
1049 483 37
BanII G_rGCy'C
Cuts at: 0 339 1215 1287 1569
Size: 339 876 72 282
Fragments arranged by size:
876 339 282 72
BclI T'GATC_A
Cuts at: 0 1499 1569
Size: 1499 70
BglI GCCn_nnn'nGGC
Cuts at: 0 334 1276 1569
Size: 334 942 293
Fragments arranged by size:
942 334 293
BlpI GC'TnA_GC
Cuts at: 0 305 1569
Size: 305 1264
Bme1580I G_kGCm'C
Cuts at: 0 256 339 1569
Size: 256 83 1230
Fragments arranged by size:
1230 256 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 573 1569
Size: 546 27 996
Fragments arranged by size:
996 546 27
BmtI G_CTAG'C
Cuts at: 0 943 1569
Size: 943 626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 1569
Size: 198 1371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 729 1569
Size: 422 307 840
Fragments arranged by size:
840 422 307
Bpu10I CC'TnA_GC
Cuts at: 0 947 1569
Size: 947 622
BsaBI GATnn'nnATC
Cuts at: 0 1014 1569
Size: 1014 555
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1394 1424 1569
Size: 874 30 490 30 145
Fragments arranged by size:
874 490 145 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 895 1569
Size: 248 647 674
Fragments arranged by size:
674 647 248
BseYI C'CCAG_C
Cuts at: 0 391 612 1143 1509 1569
Size: 391 221 531 366 60
Fragments arranged by size:
531 391 366 221 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 1569
Size: 1360 209
BsiHKAI G_wGCw'C
Cuts at: 0 1184 1215 1569
Size: 1184 31 354
Fragments arranged by size:
1184 354 31
BsmI GAATG_Cn'
Cuts at: 0 242 1569
Size: 242 1327
BsmBI CGTCTCn'nnnn_
Cuts at: 0 433 1569
Size: 433 1136
Bsp1286I G_dGCh'C
Cuts at: 0 256 339 1184 1215 1287 1569
Size: 256 83 845 31 72 282
Fragments arranged by size:
845 282 256 83 72 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 693 1163 1569
Size: 693 470 406
Fragments arranged by size:
693 470 406
BsrBI CCG'CTC
Cuts at: 0 333 1569
Size: 333 1236
BsrDI GCAATG_nn'
Cuts at: 0 1396 1569
Size: 1396 173
BsrFI r'CCGG_y
Cuts at: 0 1058 1088 1569
Size: 1058 30 481
Fragments arranged by size:
1058 481 30
BstYI r'GATC_y
Cuts at: 0 37 520 750 1491 1569
Size: 37 483 230 741 78
Fragments arranged by size:
741 483 230 78 37
Bsu36I CC'TnA_GG
Cuts at: 0 380 414 443 1569
Size: 380 34 29 1126
Fragments arranged by size:
1126 380 34 29
BtgI C'CryG_G
Cuts at: 0 14 617 1093 1569
Size: 14 603 476 476
Fragments arranged by size:
603 476 476 14
BtsI GCAGTG_nn'
Cuts at: 0 83 1566 1569
Size: 83 1483 3
Fragments arranged by size:
1483 83 3
EaeI y'GGCC_r
Cuts at: 0 11 1090 1226 1569
Size: 11 1079 136 343
Fragments arranged by size:
1079 343 136 11
EarI CTCTTCn'nnn_
Cuts at: 0 541 872 1569
Size: 541 331 697
Fragments arranged by size:
697 541 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 1569
Size: 67 1502
EcoICRI GAG'CTC
Cuts at: 0 1213 1569
Size: 1213 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 729 1021 1569
Size: 422 307 292 548
Fragments arranged by size:
548 422 307 292
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 841 1569
Size: 343 498 728
Fragments arranged by size:
728 498 343
EcoO109I rG'GnC_Cy
Cuts at: 0 336 648 1569
Size: 336 312 921
Fragments arranged by size:
921 336 312
EcoRI G'AATT_C
Cuts at: 0 1031 1569
Size: 1031 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 505 512 537 544 874 906
Size: 22 32 451 7 25 7 330 32
Cuts at: 906 980 1012 1569
Size: 74 32 557
Fragments arranged by size:
557 451 330 74 32 32 32 25
22 7 7
HincII GTy'rAC
Cuts at: 0 493 1569
Size: 493 1076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1569
Size: 262 1307
MscI TGG'CCA
Cuts at: 0 1228 1569
Size: 1228 341
MslI CAynn'nnrTG
Cuts at: 0 856 1136 1569
Size: 856 280 433
Fragments arranged by size:
856 433 280
MspA1I CmG'CkG
Cuts at: 0 277 400 1569
Size: 277 123 1169
Fragments arranged by size:
1169 277 123
NaeI GCC'GGC
Cuts at: 0 1060 1090 1569
Size: 1060 30 479
Fragments arranged by size:
1060 479 30
NcoI C'CATG_G
Cuts at: 0 617 1093 1569
Size: 617 476 476
Fragments arranged by size:
617 476 476
NgoMIV G'CCGG_C
Cuts at: 0 1058 1088 1569
Size: 1058 30 481
Fragments arranged by size:
1058 481 30
NheI G'CTAG_C
Cuts at: 0 939 1569
Size: 939 630
NruI TCG'CGA
Cuts at: 0 26 1569
Size: 26 1543
NspI r_CATG'y
Cuts at: 0 819 1411 1569
Size: 819 592 158
Fragments arranged by size:
819 592 158
PasI CC'CwG_GG
Cuts at: 0 64 645 1569
Size: 64 581 924
Fragments arranged by size:
924 581 64
PciI A'CATG_T
Cuts at: 0 1407 1569
Size: 1407 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 178 651 1569
Size: 178 473 918
Fragments arranged by size:
918 473 178
PfoI T'CCnGG_A
Cuts at: 0 40 1569
Size: 40 1529
PspOMI G'GGCC_C
Cuts at: 0 335 1569
Size: 335 1234
PspXI vC'TCGA_Gb
Cuts at: 0 1280 1569
Size: 1280 289
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1526 1558 1569
Size: 1526 32 11
Fragments arranged by size:
1526 32 11
PstI C_TGCA'G
Cuts at: 0 113 198 638 1170 1569
Size: 113 85 440 532 399
Fragments arranged by size:
532 440 399 113 85
PvuII CAG'CTG
Cuts at: 0 277 400 1569
Size: 277 123 1169
Fragments arranged by size:
1169 277 123
SacI G_AGCT'C
Cuts at: 0 1215 1569
Size: 1215 354
ScaI AGT'ACT
Cuts at: 0 640 1569
Size: 640 929
SfcI C'TryA_G
Cuts at: 0 109 194 634 1166 1569
Size: 109 85 440 532 403
Fragments arranged by size:
532 440 403 109 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 334 1569
Size: 334 1235
SmlI C'TyrA_G
Cuts at: 0 1280 1569
Size: 1280 289
SphI G_CATG'C
Cuts at: 0 819 1569
Size: 819 750
StyI C'CwwG_G
Cuts at: 0 617 1093 1196 1569
Size: 617 476 103 373
Fragments arranged by size:
617 476 373 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 626 1569
Size: 626 943
TatI w'GTAC_w
Cuts at: 0 554 638 1569
Size: 554 84 931
Fragments arranged by size:
931 554 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1203 1569
Size: 1203 366
XhoI C'TCGA_G
Cuts at: 0 1280 1569
Size: 1280 289
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanII BclI BglI BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstYI Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PspOMI PspXI
PsrI PstI PvuII SacI ScaI SfcI SfiI SmlI
SphI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglII BmgBI BpuEI
BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NotI NsiI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XmaI XmnI ZraI