Restriction map for
pFN21AE5648
(Linear) (Six-base) MAPSORT of: KIEE5648.seq Check: 5899 from: 1 to: 1455
KIEE5648.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 32 1455
Size: 32 1423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 725 801 1455
Size: 417 308 76 654
Fragments arranged by size:
654 417 308 76
AflIII A'CryG_T
Cuts at: 0 938 1455
Size: 938 517
AlwNI CAG_nnn'CTG
Cuts at: 0 26 830 1455
Size: 26 804 625
Fragments arranged by size:
804 625 26
AseI AT'TA_AT
Cuts at: 0 1354 1455
Size: 1354 101
AvaI C'yCGr_G
Cuts at: 0 148 178 418 1455
Size: 148 30 240 1037
Fragments arranged by size:
1037 240 148 30
AvrII C'CTAG_G
Cuts at: 0 915 1455
Size: 915 540
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 925 958 1455
Size: 925 33 497
Fragments arranged by size:
925 497 33
BanI G'GyrC_C
Cuts at: 0 44 281 562 1455
Size: 44 237 281 893
Fragments arranged by size:
893 281 237 44
BanII G_rGCy'C
Cuts at: 0 149 1455
Size: 149 1306
BbsI GAAGACnn'nnnn_
Cuts at: 0 706 1455
Size: 706 749
BciVI GTATCCnnnnn_n'
Cuts at: 0 953 1455
Size: 953 502
BfrBI ATG'CAT
Cuts at: 0 1398 1455
Size: 1398 57
BglI GCCn_nnn'nGGC
Cuts at: 0 22 290 1455
Size: 22 268 1165
Fragments arranged by size:
1165 268 22
BglII A'GATC_T
Cuts at: 0 212 1455
Size: 212 1243
BlpI GC'TnA_GC
Cuts at: 0 256 1455
Size: 256 1199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 763 1455
Size: 206 557 692
Fragments arranged by size:
692 557 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1455
Size: 532 923
BsaHI Gr'CG_yC
Cuts at: 0 386 1455
Size: 386 1069
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 113 143 1455
Size: 113 30 1312
Fragments arranged by size:
1312 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 719 1455
Size: 719 736
BsiHKAI G_wGCw'C
Cuts at: 0 149 167 1455
Size: 149 18 1288
Fragments arranged by size:
1288 149 18
Bsp1286I G_dGCh'C
Cuts at: 0 149 167 1455
Size: 149 18 1288
Fragments arranged by size:
1288 149 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 360 1455
Size: 32 328 1095
Fragments arranged by size:
1095 328 32
BsrDI GCAATG_nn'
Cuts at: 0 957 1455
Size: 957 498
BssSI C'ACGA_G
Cuts at: 0 8 1455
Size: 8 1447
BstBI TT'CG_AA
Cuts at: 0 1288 1455
Size: 1288 167
BstYI r'GATC_y
Cuts at: 0 212 591 1455
Size: 212 379 864
Fragments arranged by size:
864 379 212
BtsI GCAGTG_nn'
Cuts at: 0 831 1455
Size: 831 624
DrdI GACnn_nn'nnGTC
Cuts at: 0 1193 1455
Size: 1193 262
EaeI y'GGCC_r
Cuts at: 0 329 969 1455
Size: 329 640 486
Fragments arranged by size:
640 486 329
EarI CTCTTCn'nnn_
Cuts at: 0 275 1158 1318 1455
Size: 275 883 160 137
Fragments arranged by size:
883 275 160 137
EcoICRI GAG'CTC
Cuts at: 0 147 1455
Size: 147 1308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 417 725 763 801 1455
Size: 206 211 308 38 38 654
Fragments arranged by size:
654 308 211 206 38 38
EcoO109I rG'GnC_Cy
Cuts at: 0 23 406 1020 1168 1455
Size: 23 383 614 148 287
Fragments arranged by size:
614 383 287 148 23
HaeII r_GCGC'y
Cuts at: 0 205 1455
Size: 205 1250
HincII GTy'rAC
Cuts at: 0 98 1455
Size: 98 1357
HindIII A'AGCT_T
Cuts at: 0 707 1455
Size: 707 748
MslI CAynn'nnrTG
Cuts at: 0 1381 1455
Size: 1381 74
MspA1I CmG'CkG
Cuts at: 0 1065 1455
Size: 1065 390
NsiI A_TGCA'T
Cuts at: 0 1400 1455
Size: 1400 55
NspI r_CATG'y
Cuts at: 0 130 942 1038 1455
Size: 130 812 96 417
Fragments arranged by size:
812 417 130 96
PasI CC'CwG_GG
Cuts at: 0 881 1455
Size: 881 574
PciI A'CATG_T
Cuts at: 0 938 1455
Size: 938 517
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1455
Size: 1020 435
PspXI vC'TCGA_Gb
Cuts at: 0 148 1455
Size: 148 1307
PstI C_TGCA'G
Cuts at: 0 43 450 1455
Size: 43 407 1005
Fragments arranged by size:
1005 407 43
PvuII CAG'CTG
Cuts at: 0 1065 1455
Size: 1065 390
SacI G_AGCT'C
Cuts at: 0 149 1455
Size: 149 1306
SfcI C'TryA_G
Cuts at: 0 39 446 1455
Size: 39 407 1009
Fragments arranged by size:
1009 407 39
SmaI CCC'GGG
Cuts at: 0 180 420 1455
Size: 180 240 1035
Fragments arranged by size:
1035 240 180
SmlI C'TyrA_G
Cuts at: 0 148 511 1455
Size: 148 363 944
Fragments arranged by size:
944 363 148
SphI G_CATG'C
Cuts at: 0 1038 1455
Size: 1038 417
StyI C'CwwG_G
Cuts at: 0 302 566 915 1455
Size: 302 264 349 540
Fragments arranged by size:
540 349 302 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 182 269 1043 1455
Size: 182 87 774 412
Fragments arranged by size:
774 412 182 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 1455
Size: 113 32 1310
Fragments arranged by size:
1310 113 32
XhoI C'TCGA_G
Cuts at: 0 148 1455
Size: 148 1307
XmaI C'CCGG_G
Cuts at: 0 178 418 1455
Size: 178 240 1037
Fragments arranged by size:
1037 240 178
Enzymes that do cut:
AarI AcuI AflIII AlwNI AseI AvaI AvrII BaeI
BanI BanII BbsI BciVI BfrBI BglI BglII BlpI
BpmI BpuEI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BsrDI BssSI BstBI BstYI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII HincII HindIII MslI MspA1I
NsiI NspI PasI PciI PpuMI PspXI PstI PvuII
SacI SfcI SmaI SmlI SphI StyI TaqII TstI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI
BamHI BbeI BbvCI BcgI BclI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XmnI ZraI