Restriction map for
pFN21AE5646
(Linear) (Six-base) MAPSORT of: KIEE5646.seq Check: 6877 from: 1 to: 1476
KIEE5646.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AatII G_ACGT'C
Cuts at: 0 35 1476
Size: 35 1441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1476
Size: 1199 277
AflIII A'CryG_T
Cuts at: 0 933 1476
Size: 933 543
AlwNI CAG_nnn'CTG
Cuts at: 0 175 1476
Size: 175 1301
ApoI r'AATT_y
Cuts at: 0 78 859 1476
Size: 78 781 617
Fragments arranged by size:
781 617 78
AseI AT'TA_AT
Cuts at: 0 416 517 1476
Size: 416 101 959
Fragments arranged by size:
959 416 101
AvaI C'yCGr_G
Cuts at: 0 1337 1476
Size: 1337 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 832 1121 1476
Size: 832 289 355
Fragments arranged by size:
832 355 289
BbvCI CC'TCA_GC
Cuts at: 0 614 1476
Size: 614 862
BciVI GTATCCnnnnn_n'
Cuts at: 0 1071 1476
Size: 1071 405
BclI T'GATC_A
Cuts at: 0 216 303 1476
Size: 216 87 1173
Fragments arranged by size:
1173 216 87
BglII A'GATC_T
Cuts at: 0 1389 1476
Size: 1389 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 739 1476
Size: 739 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 856 1476
Size: 856 620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 695 1476
Size: 34 661 781
Fragments arranged by size:
781 661 34
Bpu10I CC'TnA_GC
Cuts at: 0 614 1476
Size: 614 862
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 415 677 1298 1415 1476
Size: 10 405 262 621 117 61
Fragments arranged by size:
621 405 262 117 61 10
BsaBI GATnn'nnATC
Cuts at: 0 761 1476
Size: 761 715
BsaHI Gr'CG_yC
Cuts at: 0 32 1476
Size: 32 1444
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 1476
Size: 200 1276
BsiEI CG_ry'CG
Cuts at: 0 64 1476
Size: 64 1412
BsmI GAATG_Cn'
Cuts at: 0 889 1476
Size: 889 587
BsrGI T'GTAC_A
Cuts at: 0 47 132 1476
Size: 47 85 1344
Fragments arranged by size:
1344 85 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 211 1476
Size: 211 1265
BstYI r'GATC_y
Cuts at: 0 313 762 1389 1476
Size: 313 449 627 87
Fragments arranged by size:
627 449 313 87
BtgI C'CryG_G
Cuts at: 0 180 284 1476
Size: 180 104 1192
Fragments arranged by size:
1192 180 104
BtsI GCAGTG_nn'
Cuts at: 0 306 1476
Size: 306 1170
ClaI AT'CG_AT
Cuts at: 0 528 1476
Size: 528 948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1025 1060 1476
Size: 1025 35 416
Fragments arranged by size:
1025 416 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 834 1476
Size: 834 642
EaeI y'GGCC_r
Cuts at: 0 61 177 201 1350 1476
Size: 61 116 24 1149 126
Fragments arranged by size:
1149 126 116 61 24
EagI C'GGCC_G
Cuts at: 0 61 1476
Size: 61 1415
EarI CTCTTCn'nnn_
Cuts at: 0 1175 1476
Size: 1175 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 695 1199 1476
Size: 34 661 504 277
Fragments arranged by size:
661 504 277 34
EcoRV GAT'ATC
Cuts at: 0 459 1230 1476
Size: 459 771 246
Fragments arranged by size:
771 459 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 1108 1140 1476
Size: 179 32 897 32 336
Fragments arranged by size:
897 336 179 32 32
HincII GTy'rAC
Cuts at: 0 432 1476
Size: 432 1044
HindIII A'AGCT_T
Cuts at: 0 225 1476
Size: 225 1251
MscI TGG'CCA
Cuts at: 0 203 1352 1476
Size: 203 1149 124
Fragments arranged by size:
1149 203 124
MspA1I CmG'CkG
Cuts at: 0 613 1476
Size: 613 863
NcoI C'CATG_G
Cuts at: 0 284 1476
Size: 284 1192
NspI r_CATG'y
Cuts at: 0 937 1206 1476
Size: 937 269 270
Fragments arranged by size:
937 270 269
PciI A'CATG_T
Cuts at: 0 933 1476
Size: 933 543
PvuII CAG'CTG
Cuts at: 0 613 1476
Size: 613 863
SfcI C'TryA_G
Cuts at: 0 167 1233 1476
Size: 167 1066 243
Fragments arranged by size:
1066 243 167
SmlI C'TyrA_G
Cuts at: 0 1337 1476
Size: 1337 139
SspI AAT'ATT
Cuts at: 0 106 776 1476
Size: 106 670 700
Fragments arranged by size:
700 670 106
StyI C'CwwG_G
Cuts at: 0 284 1476
Size: 284 1192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1322 1439 1476
Size: 1322 117 37
Fragments arranged by size:
1322 117 37
TatI w'GTAC_w
Cuts at: 0 47 132 720 1476
Size: 47 85 588 756
Fragments arranged by size:
756 588 85 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 165 197 1476
Size: 165 32 1279
Fragments arranged by size:
1279 165 32
Tth111I GACn'n_nGTC
Cuts at: 0 34 1476
Size: 34 1442
XbaI T'CTAG_A
Cuts at: 0 345 781 1476
Size: 345 436 695
Fragments arranged by size:
695 436 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 373 1476
Size: 373 1103
XhoI C'TCGA_G
Cuts at: 0 1337 1476
Size: 1337 139
XmnI GAAnn'nnTTC
Cuts at: 0 889 1476
Size: 889 587
ZraI GAC'GTC
Cuts at: 0 33 1476
Size: 33 1443
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApoI AseI AvaI BbsI
BbvCI BciVI BclI BglII BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BseRI BsiEI BsmI BsrGI BstXI
BstYI BtgI BtsI ClaI CspCI DrdI EaeI EagI
EarI Eco57MI EcoRV Hin4I HincII HindIII MscI MspA1I
NcoI NspI PciI PvuII SfcI SmlI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmtI BpuEI BsaAI BsaWI BsaXI
BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI DraI DraIII EciI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI XmaI