Restriction map for
pFN21AE5645
(Linear) (Six-base) MAPSORT of: KIEE5645.seq Check: 5181 from: 1 to: 1440
KIEE5645.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1375 1440
Size: 1013 362 65
Fragments arranged by size:
1013 362 65
AleI CACnn'nnGTG
Cuts at: 0 623 1440
Size: 623 817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 1440
Size: 358 32 1050
Fragments arranged by size:
1050 358 32
AseI AT'TA_AT
Cuts at: 0 857 1440
Size: 857 583
AvaI C'yCGr_G
Cuts at: 0 223 1440
Size: 223 1217
BamHI G'GATC_C
Cuts at: 0 997 1440
Size: 997 443
BanI G'GyrC_C
Cuts at: 0 1239 1440
Size: 1239 201
BanII G_rGCy'C
Cuts at: 0 1019 1145 1440
Size: 1019 126 295
Fragments arranged by size:
1019 295 126
BbeI G_GCGC'C
Cuts at: 0 1243 1440
Size: 1243 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 349 1020 1440
Size: 125 224 671 420
Fragments arranged by size:
671 420 224 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 767 1440
Size: 733 34 673
Fragments arranged by size:
733 673 34
BclI T'GATC_A
Cuts at: 0 1058 1440
Size: 1058 382
BlpI GC'TnA_GC
Cuts at: 0 725 1440
Size: 725 715
Bme1580I G_kGCm'C
Cuts at: 0 1266 1440
Size: 1266 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1440
Size: 358 1082
Bpu10I CC'TnA_GC
Cuts at: 0 22 1440
Size: 22 1418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1239 1440
Size: 260 979 201
Fragments arranged by size:
979 260 201
BsaBI GATnn'nnATC
Cuts at: 0 242 1440
Size: 242 1198
BsaHI Gr'CG_yC
Cuts at: 0 1240 1440
Size: 1240 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1440
Size: 478 30 932
Fragments arranged by size:
932 478 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 1246 1249 1440
Size: 502 744 3 191
Fragments arranged by size:
744 502 191 3
BseYI C'CCAG_C
Cuts at: 0 1330 1440
Size: 1330 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1440
Size: 68 1372
BsiHKAI G_wGCw'C
Cuts at: 0 28 620 717 1440
Size: 28 592 97 723
Fragments arranged by size:
723 592 97 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1335 1440
Size: 1335 105
Bsp1286I G_dGCh'C
Cuts at: 0 28 620 717 1019 1145 1266 1440
Size: 28 592 97 302 126 121 174
Fragments arranged by size:
592 302 174 126 121 97 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 1408 1440
Size: 362 1046 32
Fragments arranged by size:
1046 362 32
BsrGI T'GTAC_A
Cuts at: 0 303 317 1440
Size: 303 14 1123
Fragments arranged by size:
1123 303 14
BstBI TT'CG_AA
Cuts at: 0 543 1440
Size: 543 897
BstYI r'GATC_y
Cuts at: 0 997 1440
Size: 997 443
BtgI C'CryG_G
Cuts at: 0 843 1145 1440
Size: 843 302 295
Fragments arranged by size:
843 302 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 395 1440
Size: 395 1045
BtsI GCAGTG_nn'
Cuts at: 0 987 1440
Size: 987 453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 990 1025 1440
Size: 990 35 415
Fragments arranged by size:
990 415 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 970 1440
Size: 970 470
EaeI y'GGCC_r
Cuts at: 0 556 1440
Size: 556 884
EarI CTCTTCn'nnn_
Cuts at: 0 473 1333 1379 1413 1440
Size: 473 860 46 34 27
Fragments arranged by size:
860 473 46 34 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1013 1375 1440
Size: 358 655 362 65
Fragments arranged by size:
655 362 358 65
EcoO109I rG'GnC_Cy
Cuts at: 0 228 1440
Size: 228 1212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 569 1440
Size: 569 871
HaeII r_GCGC'y
Cuts at: 0 1243 1440
Size: 1243 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 478 510 1440
Size: 60 32 386 32 930
Fragments arranged by size:
930 386 60 32 32
HincII GTy'rAC
Cuts at: 0 387 1440
Size: 387 1053
KasI G'GCGC_C
Cuts at: 0 1239 1440
Size: 1239 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1440
Size: 528 912
MscI TGG'CCA
Cuts at: 0 558 1440
Size: 558 882
MslI CAynn'nnrTG
Cuts at: 0 623 842 1440
Size: 623 219 598
Fragments arranged by size:
623 598 219
NarI GG'CG_CC
Cuts at: 0 1240 1440
Size: 1240 200
NcoI C'CATG_G
Cuts at: 0 843 1145 1440
Size: 843 302 295
Fragments arranged by size:
843 302 295
NspI r_CATG'y
Cuts at: 0 393 1440
Size: 393 1047
PsiI TTA'TAA
Cuts at: 0 422 1440
Size: 422 1018
PspXI vC'TCGA_Gb
Cuts at: 0 223 1440
Size: 223 1217
PstI C_TGCA'G
Cuts at: 0 1405 1440
Size: 1405 35
SapI GCTCTTCn'nnn_
Cuts at: 0 1379 1440
Size: 1379 61
SexAI A'CCwGG_T
Cuts at: 0 293 1440
Size: 293 1147
SfcI C'TryA_G
Cuts at: 0 1401 1440
Size: 1401 39
SfoI GGC'GCC
Cuts at: 0 1241 1440
Size: 1241 199
SmlI C'TyrA_G
Cuts at: 0 223 275 1254 1440
Size: 223 52 979 186
Fragments arranged by size:
979 223 186 52
StyI C'CwwG_G
Cuts at: 0 843 1145 1440
Size: 843 302 295
Fragments arranged by size:
843 302 295
TatI w'GTAC_w
Cuts at: 0 303 317 1309 1440
Size: 303 14 992 131
Fragments arranged by size:
992 303 131 14
XbaI T'CTAG_A
Cuts at: 0 1303 1440
Size: 1303 137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 766 1440
Size: 766 674
XhoI C'TCGA_G
Cuts at: 0 223 1440
Size: 223 1217
XmnI GAAnn'nnTTC
Cuts at: 0 1388 1440
Size: 1388 52
Enzymes that do cut:
AcuI AleI AloI AseI AvaI BamHI BanI BanII
BbeI BbsI BcgI BclI BlpI Bme1580I BpmI Bpu10I
BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrGI BstBI BstYI BtgI BtgZI
BtsI CspCI DrdI EaeI EarI Eco57MI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MslI NarI
NcoI NspI PsiI PspXI PstI SapI SexAI SfcI
SfoI SmlI StyI TatI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BbvCI BciVI BfrBI BglI BglII
BmgBI BmrI BmtI BplI BsaI BsaAI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I ClaI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XmaI ZraI