Restriction map for
pFN21AE5644
(Linear) (Six-base) MAPSORT of: KIEE5644.seq Check: 4066 from: 1 to: 1431
KIEE5644.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 123 1431
Size: 123 1308
AflII C'TTAA_G
Cuts at: 0 243 1431
Size: 243 1188
AleI CACnn'nnGTG
Cuts at: 0 508 1431
Size: 508 923
AlwNI CAG_nnn'CTG
Cuts at: 0 208 786 840 1431
Size: 208 578 54 591
Fragments arranged by size:
591 578 208 54
ApoI r'AATT_y
Cuts at: 0 1123 1431
Size: 1123 308
BamHI G'GATC_C
Cuts at: 0 592 752 988 1431
Size: 592 160 236 443
Fragments arranged by size:
592 443 236 160
BanI G'GyrC_C
Cuts at: 0 123 1431
Size: 123 1308
BanII G_rGCy'C
Cuts at: 0 1253 1431
Size: 1253 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 1431
Size: 323 1108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 536 1431
Size: 502 34 895
Fragments arranged by size:
895 502 34
BclI T'GATC_A
Cuts at: 0 495 1431
Size: 495 936
Bme1580I G_kGCm'C
Cuts at: 0 39 1431
Size: 39 1392
BmrI ACTGGGnnnn_n'
Cuts at: 0 751 850 1431
Size: 751 99 581
Fragments arranged by size:
751 581 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 936 1296 1348 1431
Size: 814 122 360 52 83
Fragments arranged by size:
814 360 122 83 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 251 275 577 848 1318 1431
Size: 119 132 24 302 271 470 113
Fragments arranged by size:
470 302 271 132 119 113 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 1431
Size: 535 896
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 722 752 1294 1324 1431
Size: 107 30 585 30 542 30 107
Fragments arranged by size:
585 542 107 107 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 553 1431
Size: 133 420 878
Fragments arranged by size:
878 420 133
BseYI C'CCAG_C
Cuts at: 0 38 1359 1431
Size: 38 1321 72
Fragments arranged by size:
1321 72 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 532 1431
Size: 194 338 899
Fragments arranged by size:
899 338 194
BsiHKAI G_wGCw'C
Cuts at: 0 308 1431
Size: 308 1123
BsmI GAATG_Cn'
Cuts at: 0 884 1431
Size: 884 547
Bsp1286I G_dGCh'C
Cuts at: 0 39 308 1253 1431
Size: 39 269 945 178
Fragments arranged by size:
945 269 178 39
BspHI T'CATG_A
Cuts at: 0 806 1431
Size: 806 625
BsrBI CCG'CTC
Cuts at: 0 1370 1431
Size: 1370 61
BssSI C'ACGA_G
Cuts at: 0 743 1431
Size: 743 688
BstEII G'GTnAC_C
Cuts at: 0 619 1431
Size: 619 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 485 1282 1320 1431
Size: 485 797 38 111
Fragments arranged by size:
797 485 111 38
BstYI r'GATC_y
Cuts at: 0 4 204 592 752 931 988 1431
Size: 4 200 388 160 179 57 443
Fragments arranged by size:
443 388 200 179 160 57 4
Bsu36I CC'TnA_GG
Cuts at: 0 536 1431
Size: 536 895
BtgI C'CryG_G
Cuts at: 0 1412 1431
Size: 1412 19
DraI TTT'AAA
Cuts at: 0 1128 1431
Size: 1128 303
EaeI y'GGCC_r
Cuts at: 0 631 1431
Size: 631 800
EarI CTCTTCn'nnn_
Cuts at: 0 1013 1241 1431
Size: 1013 228 190
Fragments arranged by size:
1013 228 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 936 1296 1348 1431
Size: 814 122 360 52 83
Fragments arranged by size:
814 360 122 83 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 659 1273 1431
Size: 659 614 158
Fragments arranged by size:
659 614 158
EcoO109I rG'GnC_Cy
Cuts at: 0 1111 1223 1431
Size: 1111 112 208
Fragments arranged by size:
1111 208 112
HaeII r_GCGC'y
Cuts at: 0 1420 1431
Size: 1420 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 584 616 846 878 1294 1326
Size: 472 32 80 32 230 32 416 32
Cuts at: 1326 1431
Size: 105
Fragments arranged by size:
472 416 230 105 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 762 900 1431
Size: 762 138 531
Fragments arranged by size:
762 531 138
HpaI GTT'AAC
Cuts at: 0 900 1431
Size: 900 531
KpnI G_GTAC'C
Cuts at: 0 127 1431
Size: 127 1304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1431
Size: 1004 427
MscI TGG'CCA
Cuts at: 0 633 1431
Size: 633 798
MslI CAynn'nnrTG
Cuts at: 0 508 1431
Size: 508 923
NspI r_CATG'y
Cuts at: 0 768 1431
Size: 768 663
PflMI CCAn_nnn'nTGG
Cuts at: 0 430 602 840 1431
Size: 430 172 238 591
Fragments arranged by size:
591 430 238 172
PfoI T'CCnGG_A
Cuts at: 0 779 1431
Size: 779 652
PstI C_TGCA'G
Cuts at: 0 832 1382 1431
Size: 832 550 49
Fragments arranged by size:
832 550 49
SapI GCTCTTCn'nnn_
Cuts at: 0 1241 1431
Size: 1241 190
SfcI C'TryA_G
Cuts at: 0 828 1378 1431
Size: 828 550 53
Fragments arranged by size:
828 550 53
SmlI C'TyrA_G
Cuts at: 0 134 243 266 290 556 863 1297 1431
Size: 134 109 23 24 266 307 434 134
Fragments arranged by size:
434 307 266 134 134 109 24 23
SspI AAT'ATT
Cuts at: 0 670 1431
Size: 670 761
StuI AGG'CCT
Cuts at: 0 1147 1431
Size: 1147 284
StyI C'CwwG_G
Cuts at: 0 1148 1431
Size: 1148 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 335 1431
Size: 335 1096
XmnI GAAnn'nnTTC
Cuts at: 0 671 1431
Size: 671 760
Enzymes that do cut:
Acc65I AflII AleI AlwNI ApoI BamHI BanI BanII
BbsI BcgI BclI Bme1580I BmrI BpmI BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BssSI BstEII BstXI BstYI Bsu36I BtgI DraI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HpaI KpnI MmeI MscI MslI NspI PflMI PfoI
PstI SapI SfcI SmlI SspI StuI StyI TaqII
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII KasI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI