Restriction map for
pFN21AE5639
(Linear) (Six-base) MAPSORT of: KIEE5639.seq Check: 7603 from: 1 to: 1686
KIEE5639.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AatII G_ACGT'C
Cuts at: 0 525 1686
Size: 525 1161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1085 1268 1319 1686
Size: 703 382 183 51 367
Fragments arranged by size:
703 382 367 183 51
AlwNI CAG_nnn'CTG
Cuts at: 0 151 171 390 412 642 765 849 966
Size: 151 20 219 22 230 123 84 117
Cuts at: 966 996 1576 1686
Size: 30 580 110
Fragments arranged by size:
580 230 219 151 123 117 110 84
30 22 20
ApaI G_GGCC'C
Cuts at: 0 19 205 262 1052 1074 1686
Size: 19 186 57 790 22 612
Fragments arranged by size:
790 612 186 57 22 19
AvaI C'yCGr_G
Cuts at: 0 460 1686
Size: 460 1226
BanI G'GyrC_C
Cuts at: 0 1470 1686
Size: 1470 216
BanII G_rGCy'C
Cuts at: 0 19 205 262 375 512 1052 1074 1401
Size: 19 186 57 113 137 540 22 327
Cuts at: 1401 1686
Size: 285
Fragments arranged by size:
540 327 285 186 137 113 57 22
19
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 691 1686
Size: 455 236 995
Fragments arranged by size:
995 455 236
BbvCI CC'TCA_GC
Cuts at: 0 413 569 1686
Size: 413 156 1117
Fragments arranged by size:
1117 413 156
BglI GCCn_nnn'nGGC
Cuts at: 0 742 1686
Size: 742 944
BglII A'GATC_T
Cuts at: 0 599 1686
Size: 599 1087
BlpI GC'TnA_GC
Cuts at: 0 487 511 1686
Size: 487 24 1175
Fragments arranged by size:
1175 487 24
Bme1580I G_kGCm'C
Cuts at: 0 19 205 262 1052 1074 1686
Size: 19 186 57 790 22 612
Fragments arranged by size:
790 612 186 57 22 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 1489 1686
Size: 1489 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 451 517 526 694 724 1024 1039
Size: 202 249 66 9 168 30 300 15
Cuts at: 1039 1415 1430 1441 1639 1654 1664 1686
Size: 376 15 11 198 15 10 22
Fragments arranged by size:
376 300 249 202 198 168 66 30
22 15 15 15 11 10 9
Bpu10I CC'TnA_GC
Cuts at: 0 413 569 1686
Size: 413 156 1117
Fragments arranged by size:
1117 413 156
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 1213 1431 1686
Size: 349 864 218 255
Fragments arranged by size:
864 349 255 218
BsaHI Gr'CG_yC
Cuts at: 0 522 1686
Size: 522 1164
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 112 367 430 586 610 682 808
Size: 79 33 255 63 156 24 72 126
Cuts at: 808 1036 1363 1518 1686
Size: 228 327 155 168
Fragments arranged by size:
327 255 228 168 156 155 126 79
72 63 33 24
BseYI C'CCAG_C
Cuts at: 0 75 383 632 1073 1116 1254 1258 1569
Size: 75 308 249 441 43 138 4 311
Cuts at: 1569 1686
Size: 117
Fragments arranged by size:
441 311 308 249 138 117 75 43
4
BsiHKAI G_wGCw'C
Cuts at: 0 512 1686
Size: 512 1174
BsmI GAATG_Cn'
Cuts at: 0 86 543 1686
Size: 86 457 1143
Fragments arranged by size:
1143 457 86
Bsp1286I G_dGCh'C
Cuts at: 0 19 205 262 375 512 1052 1074 1401
Size: 19 186 57 113 137 540 22 327
Cuts at: 1401 1686
Size: 285
Fragments arranged by size:
540 327 285 186 137 113 57 22
19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 491 1449 1686
Size: 14 477 958 237
Fragments arranged by size:
958 477 237 14
BsrBI CCG'CTC
Cuts at: 0 57 819 1686
Size: 57 762 867
Fragments arranged by size:
867 762 57
BsrFI r'CCGG_y
Cuts at: 0 84 1686
Size: 84 1602
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 1686
Size: 642 1044
BstBI TT'CG_AA
Cuts at: 0 122 1686
Size: 122 1564
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 660 702 1325 1686
Size: 240 420 42 623 361
Fragments arranged by size:
623 420 361 240 42
BstYI r'GATC_y
Cuts at: 0 599 1686
Size: 599 1087
Bsu36I CC'TnA_GG
Cuts at: 0 11 62 263 1686
Size: 11 51 201 1423
Fragments arranged by size:
1423 201 51 11
BtgI C'CryG_G
Cuts at: 0 659 854 1686
Size: 659 195 832
Fragments arranged by size:
832 659 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 956 1097 1686
Size: 956 141 589
Fragments arranged by size:
956 589 141
BtsI GCAGTG_nn'
Cuts at: 0 32 1204 1686
Size: 32 1172 482
Fragments arranged by size:
1172 482 32
EaeI y'GGCC_r
Cuts at: 0 230 692 851 1686
Size: 230 462 159 835
Fragments arranged by size:
835 462 230 159
EarI CTCTTCn'nnn_
Cuts at: 0 344 1077 1332 1340 1686
Size: 344 733 255 8 346
Fragments arranged by size:
733 346 344 255 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1686
Size: 760 926
EcoICRI GAG'CTC
Cuts at: 0 510 1686
Size: 510 1176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 451 517 526 694 703 724 1024
Size: 202 249 66 9 168 9 21 300
Cuts at: 1024 1039 1085 1268 1319 1415 1430 1441 1639
Size: 15 46 183 51 96 15 11 198
Cuts at: 1639 1654 1664 1686
Size: 15 10 22
Fragments arranged by size:
300 249 202 198 183 168 96 66
51 46 22 21 15 15 15 11
10 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 592 1301 1686
Size: 10 582 709 385
Fragments arranged by size:
709 582 385 10
EcoO109I rG'GnC_Cy
Cuts at: 0 15 201 202 258 259 501 639 678
Size: 15 186 1 56 1 242 138 39
Cuts at: 678 1048 1070 1508 1686
Size: 370 22 438 178
Fragments arranged by size:
438 370 242 186 178 138 56 39
22 15 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 591 594 623 768 800 905 937
Size: 562 29 3 29 145 32 105 32
Cuts at: 937 1228 1260 1557 1589 1686
Size: 291 32 297 32 97
Fragments arranged by size:
562 297 291 145 105 97 32 32
32 32 29 29 3
HincII GTy'rAC
Cuts at: 0 325 1331 1686
Size: 325 1006 355
Fragments arranged by size:
1006 355 325
HindIII A'AGCT_T
Cuts at: 0 125 1686
Size: 125 1561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1686
Size: 1199 487
MscI TGG'CCA
Cuts at: 0 232 694 853 1686
Size: 232 462 159 833
Fragments arranged by size:
833 462 232 159
MslI CAynn'nnrTG
Cuts at: 0 399 1323 1686
Size: 399 924 363
Fragments arranged by size:
924 399 363
MspA1I CmG'CkG
Cuts at: 0 478 552 568 1029 1077 1258 1539 1686
Size: 478 74 16 461 48 181 281 147
Fragments arranged by size:
478 461 281 181 147 74 48 16
NcoI C'CATG_G
Cuts at: 0 659 854 1686
Size: 659 195 832
Fragments arranged by size:
832 659 195
NruI TCG'CGA
Cuts at: 0 941 1686
Size: 941 745
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 228 966 1686
Size: 171 57 738 720
Fragments arranged by size:
738 720 171 57
PfoI T'CCnGG_A
Cuts at: 0 50 1686
Size: 50 1636
PpuMI rG'GwC_Cy
Cuts at: 0 501 678 1686
Size: 501 177 1008
Fragments arranged by size:
1008 501 177
PspOMI G'GGCC_C
Cuts at: 0 15 201 258 1048 1070 1686
Size: 15 186 57 790 22 616
Fragments arranged by size:
790 616 186 57 22 15
PstI C_TGCA'G
Cuts at: 0 551 647 791 1686
Size: 551 96 144 895
Fragments arranged by size:
895 551 144 96
PvuII CAG'CTG
Cuts at: 0 478 568 1029 1077 1258 1686
Size: 478 90 461 48 181 428
Fragments arranged by size:
478 461 428 181 90 48
SacI G_AGCT'C
Cuts at: 0 512 1686
Size: 512 1174
SfcI C'TryA_G
Cuts at: 0 547 643 787 1686
Size: 547 96 144 899
Fragments arranged by size:
899 547 144 96
StyI C'CwwG_G
Cuts at: 0 588 653 659 854 1686
Size: 588 65 6 195 832
Fragments arranged by size:
832 588 195 65 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 13 646 1111 1686
Size: 13 633 465 575
Fragments arranged by size:
633 575 465 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1542 1574 1686
Size: 1542 32 112
Fragments arranged by size:
1542 112 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1640 1686
Size: 276 1364 46
Fragments arranged by size:
1364 276 46
ZraI GAC'GTC
Cuts at: 0 523 1686
Size: 523 1163
Enzymes that do cut:
AatII AcuI AlwNI ApaI AvaI BanI BanII BbsI
BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I HincII HindIII MmeI MscI MslI MspA1I
NcoI NruI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SfcI StyI TaqII TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI