Restriction map for
pFN21AE5638
(Linear) (Six-base) MAPSORT of: KIEE5638.seq Check: 6881 from: 1 to: 1641
KIEE5638.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 800 1043 1600 1641
Size: 563 237 243 557 41
Fragments arranged by size:
563 557 243 237 41
AflIII A'CryG_T
Cuts at: 0 312 1641
Size: 312 1329
AleI CACnn'nnGTG
Cuts at: 0 580 1641
Size: 580 1061
AlwNI CAG_nnn'CTG
Cuts at: 0 60 1641
Size: 60 1581
ApaI G_GGCC'C
Cuts at: 0 999 1641
Size: 999 642
ApaLI G'TGCA_C
Cuts at: 0 193 1641
Size: 193 1448
ApoI r'AATT_y
Cuts at: 0 168 1495 1641
Size: 168 1327 146
Fragments arranged by size:
1327 168 146
AvrII C'CTAG_G
Cuts at: 0 1566 1641
Size: 1566 75
BamHI G'GATC_C
Cuts at: 0 1441 1641
Size: 1441 200
BanII G_rGCy'C
Cuts at: 0 742 794 999 1641
Size: 742 52 205 642
Fragments arranged by size:
742 642 205 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 629 713 1096 1641
Size: 629 84 383 545
Fragments arranged by size:
629 545 383 84
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 1641
Size: 192 1449
BglI GCCn_nnn'nGGC
Cuts at: 0 1003 1641
Size: 1003 638
Bme1580I G_kGCm'C
Cuts at: 0 197 999 1023 1254 1641
Size: 197 802 24 231 387
Fragments arranged by size:
802 387 231 197 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 752 1641
Size: 235 517 889
Fragments arranged by size:
889 517 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1603 1641
Size: 698 905 38
Fragments arranged by size:
905 698 38
BsaHI Gr'CG_yC
Cuts at: 0 1093 1641
Size: 1093 548
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 637 1054 1641
Size: 637 417 587
Fragments arranged by size:
637 587 417
BseYI C'CCAG_C
Cuts at: 0 60 488 520 640 1641
Size: 60 428 32 120 1001
Fragments arranged by size:
1001 428 120 60 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1641
Size: 536 1105
BsiHKAI G_wGCw'C
Cuts at: 0 197 1641
Size: 197 1444
BsmI GAATG_Cn'
Cuts at: 0 618 1457 1641
Size: 618 839 184
Fragments arranged by size:
839 618 184
Bsp1286I G_dGCh'C
Cuts at: 0 197 742 794 999 1023 1254 1641
Size: 197 545 52 205 24 231 387
Fragments arranged by size:
545 387 231 205 197 52 24
BspHI T'CATG_A
Cuts at: 0 1217 1641
Size: 1217 424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 1641
Size: 275 1366
BsrGI T'GTAC_A
Cuts at: 0 140 1641
Size: 140 1501
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 1641
Size: 48 1593
BstYI r'GATC_y
Cuts at: 0 503 1441 1641
Size: 503 938 200
Fragments arranged by size:
938 503 200
BtgI C'CryG_G
Cuts at: 0 20 980 1641
Size: 20 960 661
Fragments arranged by size:
960 661 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 512 1641
Size: 512 1129
DraIII CAC_nnn'GTG
Cuts at: 0 454 1641
Size: 454 1187
EaeI y'GGCC_r
Cuts at: 0 668 1641
Size: 668 973
EarI CTCTTCn'nnn_
Cuts at: 0 20 242 626 782 1332 1576 1641
Size: 20 222 384 156 550 244 65
Fragments arranged by size:
550 384 244 222 156 65 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 563 752 800 1043 1600 1641
Size: 235 328 189 48 243 557 41
Fragments arranged by size:
557 328 243 235 189 48 41
EcoO109I rG'GnC_Cy
Cuts at: 0 995 1082 1641
Size: 995 87 559
Fragments arranged by size:
995 559 87
EcoRV GAT'ATC
Cuts at: 0 1293 1641
Size: 1293 348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 49 593 1641
Size: 49 544 1048
Fragments arranged by size:
1048 544 49
HaeII r_GCGC'y
Cuts at: 0 415 1641
Size: 415 1226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 899 931 1580 1612 1641
Size: 899 32 649 32 29
Fragments arranged by size:
899 649 32 32 29
HincII GTy'rAC
Cuts at: 0 599 857 1484 1641
Size: 599 258 627 157
Fragments arranged by size:
627 599 258 157
HindIII A'AGCT_T
Cuts at: 0 303 1641
Size: 303 1338
HpaI GTT'AAC
Cuts at: 0 599 1484 1641
Size: 599 885 157
Fragments arranged by size:
885 599 157
MscI TGG'CCA
Cuts at: 0 670 1641
Size: 670 971
MslI CAynn'nnrTG
Cuts at: 0 580 1641
Size: 580 1061
NcoI C'CATG_G
Cuts at: 0 20 1641
Size: 20 1621
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 1167 1641
Size: 60 1107 474
Fragments arranged by size:
1107 474 60
PfoI T'CCnGG_A
Cuts at: 0 560 1641
Size: 560 1081
PpuMI rG'GwC_Cy
Cuts at: 0 1082 1641
Size: 1082 559
PspOMI G'GGCC_C
Cuts at: 0 995 1641
Size: 995 646
SapI GCTCTTCn'nnn_
Cuts at: 0 782 1641
Size: 782 859
SfcI C'TryA_G
Cuts at: 0 162 1641
Size: 162 1479
SmlI C'TyrA_G
Cuts at: 0 713 1618 1641
Size: 713 905 23
Fragments arranged by size:
905 713 23
SspI AAT'ATT
Cuts at: 0 1329 1641
Size: 1329 312
StuI AGG'CCT
Cuts at: 0 107 1641
Size: 107 1534
StyI C'CwwG_G
Cuts at: 0 20 734 1566 1641
Size: 20 714 832 75
Fragments arranged by size:
832 714 75 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 1641
Size: 223 1418
TatI w'GTAC_w
Cuts at: 0 140 1099 1641
Size: 140 959 542
Fragments arranged by size:
959 542 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1433 1465 1641
Size: 1433 32 176
Fragments arranged by size:
1433 176 32
Tth111I GACn'n_nGTC
Cuts at: 0 535 1641
Size: 535 1106
XbaI T'CTAG_A
Cuts at: 0 904 1641
Size: 904 737
XmnI GAAnn'nnTTC
Cuts at: 0 576 1641
Size: 576 1065
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AvrII
BamHI BanII BbsI BciVI BglI Bme1580I BpmI BpuEI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrGI BstAPI BstYI BtgI BtgZI DraIII EaeI
EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MscI MslI NcoI PflMI PfoI PpuMI
PspOMI SapI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvaI BaeI
BanI BbeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XcmI XhoI XmaI ZraI