Restriction map for
pFN21AE5635
(Linear) (Six-base) MAPSORT of: KIEE5635.seq Check: 6143 from: 1 to: 1581
KIEE5635.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AatII G_ACGT'C
Cuts at: 0 218 1581
Size: 218 1363
AccI GT'mk_AC
Cuts at: 0 737 1581
Size: 737 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 364 547 574 1581
Size: 93 271 183 27 1007
Fragments arranged by size:
1007 271 183 93 27
AflIII A'CryG_T
Cuts at: 0 386 696 707 1172 1250 1581
Size: 386 310 11 465 78 331
Fragments arranged by size:
465 386 331 310 78 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 1581
Size: 323 1258
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 1581
Size: 1023 558
ApaI G_GGCC'C
Cuts at: 0 672 1581
Size: 672 909
ApoI r'AATT_y
Cuts at: 0 1102 1581
Size: 1102 479
AvaI C'yCGr_G
Cuts at: 0 665 671 1385 1581
Size: 665 6 714 196
Fragments arranged by size:
714 665 196 6
BanII G_rGCy'C
Cuts at: 0 167 476 672 1581
Size: 167 309 196 909
Fragments arranged by size:
909 309 196 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 1581
Size: 200 1381
BbvCI CC'TCA_GC
Cuts at: 0 640 1581
Size: 640 941
BclI T'GATC_A
Cuts at: 0 312 933 1343 1581
Size: 312 621 410 238
Fragments arranged by size:
621 410 312 238
BfrBI ATG'CAT
Cuts at: 0 1266 1581
Size: 1266 315
BglII A'GATC_T
Cuts at: 0 45 1581
Size: 45 1536
BlpI GC'TnA_GC
Cuts at: 0 763 1581
Size: 763 818
Bme1580I G_kGCm'C
Cuts at: 0 672 1227 1353 1581
Size: 672 555 126 228
Fragments arranged by size:
672 555 228 126
BmgBI CAC'GTC
Cuts at: 0 180 699 1392 1581
Size: 180 519 693 189
Fragments arranged by size:
693 519 189 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1026 1093 1327 1354 1529 1581
Size: 553 473 67 234 27 175 52
Fragments arranged by size:
553 473 234 175 67 52 27
Bpu10I CC'TnA_GC
Cuts at: 0 115 640 950 1581
Size: 115 525 310 631
Fragments arranged by size:
631 525 310 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 838 1117 1581
Size: 637 201 279 464
Fragments arranged by size:
637 464 279 201
BsaI GGTCTCn'nnnn_
Cuts at: 0 758 1581
Size: 758 823
BsaAI yAC'GTr
Cuts at: 0 225 1251 1581
Size: 225 1026 330
Fragments arranged by size:
1026 330 225
BsaBI GATnn'nnATC
Cuts at: 0 286 1581
Size: 286 1295
BsaHI Gr'CG_yC
Cuts at: 0 215 449 1581
Size: 215 234 1132
Fragments arranged by size:
1132 234 215
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 409 827 1581
Size: 21 388 418 754
Fragments arranged by size:
754 418 388 21
BseYI C'CCAG_C
Cuts at: 0 19 238 477 964 1581
Size: 19 219 239 487 617
Fragments arranged by size:
617 487 239 219 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 385 666 1581
Size: 39 346 281 915
Fragments arranged by size:
915 346 281 39
BsiEI CG_ry'CG
Cuts at: 0 101 1581
Size: 101 1480
BsiHKAI G_wGCw'C
Cuts at: 0 764 1487 1573 1581
Size: 764 723 86 8
Fragments arranged by size:
764 723 86 8
BsmI GAATG_Cn'
Cuts at: 0 210 1581
Size: 210 1371
Bsp1286I G_dGCh'C
Cuts at: 0 167 476 672 764 1227 1353 1487 1573
Size: 167 309 196 92 463 126 134 86
Cuts at: 1573 1581
Size: 8
Fragments arranged by size:
463 309 196 167 134 126 92 86
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1169 1581
Size: 1169 412
BsrBI CCG'CTC
Cuts at: 0 464 1581
Size: 464 1117
BsrDI GCAATG_nn'
Cuts at: 0 305 1038 1581
Size: 305 733 543
Fragments arranged by size:
733 543 305
BsrFI r'CCGG_y
Cuts at: 0 1013 1581
Size: 1013 568
BsrGI T'GTAC_A
Cuts at: 0 1202 1581
Size: 1202 379
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1023 1581
Size: 1023 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 1581
Size: 972 609
BstYI r'GATC_y
Cuts at: 0 45 246 398 1373 1496 1581
Size: 45 201 152 975 123 85
Fragments arranged by size:
975 201 152 123 85 45
BtgI C'CryG_G
Cuts at: 0 158 1292 1581
Size: 158 1134 289
Fragments arranged by size:
1134 289 158
BtsI GCAGTG_nn'
Cuts at: 0 315 1581
Size: 315 1266
EaeI y'GGCC_r
Cuts at: 0 98 263 597 881 974 1298 1581
Size: 98 165 334 284 93 324 283
Fragments arranged by size:
334 324 284 283 165 98 93
EagI C'GGCC_G
Cuts at: 0 98 1581
Size: 98 1483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 364 547 553 574 1026 1093 1327
Size: 93 271 183 6 21 452 67 234
Cuts at: 1327 1354 1529 1581
Size: 27 175 52
Fragments arranged by size:
452 271 234 183 175 93 67 52
27 21 6
EcoO109I rG'GnC_Cy
Cuts at: 0 339 636 1196 1581
Size: 339 297 560 385
Fragments arranged by size:
560 385 339 297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 1581
Size: 1080 501
FspI TGC'GCA
Cuts at: 0 286 1581
Size: 286 1295
FspAI rTGC'GCAy
Cuts at: 0 286 1581
Size: 286 1295
HaeII r_GCGC'y
Cuts at: 0 122 523 692 1452 1581
Size: 122 401 169 760 129
Fragments arranged by size:
760 401 169 129 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 1079 1111 1581
Size: 943 32 104 32 470
Fragments arranged by size:
943 470 104 32 32
HincII GTy'rAC
Cuts at: 0 219 1581
Size: 219 1362
MscI TGG'CCA
Cuts at: 0 976 1581
Size: 976 605
MslI CAynn'nnrTG
Cuts at: 0 706 1576 1581
Size: 706 870 5
Fragments arranged by size:
870 706 5
MspA1I CmG'CkG
Cuts at: 0 275 1480 1581
Size: 275 1205 101
Fragments arranged by size:
1205 275 101
NsiI A_TGCA'T
Cuts at: 0 1268 1581
Size: 1268 313
NspI r_CATG'y
Cuts at: 0 390 711 1176 1266 1581
Size: 390 321 465 90 315
Fragments arranged by size:
465 390 321 315 90
PciI A'CATG_T
Cuts at: 0 386 707 1172 1581
Size: 386 321 465 409
Fragments arranged by size:
465 409 386 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 624 1581
Size: 624 957
PfoI T'CCnGG_A
Cuts at: 0 1166 1331 1561 1581
Size: 1166 165 230 20
Fragments arranged by size:
1166 230 165 20
PpuMI rG'GwC_Cy
Cuts at: 0 636 1196 1581
Size: 636 560 385
Fragments arranged by size:
636 560 385
PspOMI G'GGCC_C
Cuts at: 0 668 1581
Size: 668 913
PstI C_TGCA'G
Cuts at: 0 302 437 1028 1581
Size: 302 135 591 553
Fragments arranged by size:
591 553 302 135
PvuII CAG'CTG
Cuts at: 0 1480 1581
Size: 1480 101
SfcI C'TryA_G
Cuts at: 0 270 298 433 1024 1581
Size: 270 28 135 591 557
Fragments arranged by size:
591 557 270 135 28
SmaI CCC'GGG
Cuts at: 0 667 673 1387 1581
Size: 667 6 714 194
Fragments arranged by size:
714 667 194 6
SmlI C'TyrA_G
Cuts at: 0 652 853 1132 1581
Size: 652 201 279 449
Fragments arranged by size:
652 449 279 201
StyI C'CwwG_G
Cuts at: 0 998 1457 1581
Size: 998 459 124
Fragments arranged by size:
998 459 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1222 1581
Size: 486 736 359
Fragments arranged by size:
736 486 359
TatI w'GTAC_w
Cuts at: 0 50 893 1202 1581
Size: 50 843 309 379
Fragments arranged by size:
843 379 309 50
Tth111I GACn'n_nGTC
Cuts at: 0 82 1581
Size: 82 1499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 330 1581
Size: 246 84 1251
Fragments arranged by size:
1251 246 84
XmaI C'CCGG_G
Cuts at: 0 665 671 1385 1581
Size: 665 6 714 196
Fragments arranged by size:
714 665 196 6
ZraI GAC'GTC
Cuts at: 0 216 1581
Size: 216 1365
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI BanII BbsI BbvCI BclI BfrBI BglII BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtsI EaeI EagI Eco57MI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII MscI MslI MspA1I NsiI
NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SfcI SmaI SmlI StyI TaqII TatI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BglI BmrI BmtI BplI
BsaWI BsaXI BsiWI BsmBI BspEI BspHI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmnI