Restriction map for
pFN21AE5634
(Linear) (Six-base) MAPSORT of: KIEE5634.seq Check: 8520 from: 1 to: 1752
KIEE5634.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AccI GT'mk_AC
Cuts at: 0 112 143 1752
Size: 112 31 1609
Fragments arranged by size:
1609 112 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1752
Size: 935 817
AflII C'TTAA_G
Cuts at: 0 961 1752
Size: 961 791
AflIII A'CryG_T
Cuts at: 0 77 1752
Size: 77 1675
AhdI GACnn_n'nnGTC
Cuts at: 0 78 963 1509 1752
Size: 78 885 546 243
Fragments arranged by size:
885 546 243 78
AlwNI CAG_nnn'CTG
Cuts at: 0 1456 1752
Size: 1456 296
ApaLI G'TGCA_C
Cuts at: 0 1572 1752
Size: 1572 180
ApoI r'AATT_y
Cuts at: 0 586 1014 1752
Size: 586 428 738
Fragments arranged by size:
738 586 428
AvaI C'yCGr_G
Cuts at: 0 190 256 1752
Size: 190 66 1496
Fragments arranged by size:
1496 190 66
BamHI G'GATC_C
Cuts at: 0 504 1752
Size: 504 1248
BanI G'GyrC_C
Cuts at: 0 261 618 1081 1752
Size: 261 357 463 671
Fragments arranged by size:
671 463 357 261
BanII G_rGCy'C
Cuts at: 0 1495 1752
Size: 1495 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 926 1752
Size: 41 885 826
Fragments arranged by size:
885 826 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1246 1280 1587 1621 1752
Size: 1246 34 307 34 131
Fragments arranged by size:
1246 307 131 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1734 1752
Size: 1734 18
BglI GCCn_nnn'nGGC
Cuts at: 0 649 1752
Size: 649 1103
Bme1580I G_kGCm'C
Cuts at: 0 467 1043 1576 1752
Size: 467 576 533 176
Fragments arranged by size:
576 533 467 176
BmrI ACTGGGnnnn_n'
Cuts at: 0 1563 1714 1752
Size: 1563 151 38
Fragments arranged by size:
1563 151 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1512 1752
Size: 1512 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 555 823 1389 1752
Size: 368 187 268 566 363
Fragments arranged by size:
566 368 363 268 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1688 1752
Size: 1688 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 317 988 1626 1752
Size: 317 671 638 126
Fragments arranged by size:
671 638 317 126
BsaWI w'CCGG_w
Cuts at: 0 319 420 1752
Size: 319 101 1332
Fragments arranged by size:
1332 319 101
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 557 1189 1485 1752
Size: 557 632 296 267
Fragments arranged by size:
632 557 296 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1752
Size: 1253 499
BsiHKAI G_wGCw'C
Cuts at: 0 1473 1576 1752
Size: 1473 103 176
Fragments arranged by size:
1473 176 103
Bsp1286I G_dGCh'C
Cuts at: 0 467 1043 1473 1495 1576 1752
Size: 467 576 430 22 81 176
Fragments arranged by size:
576 467 430 176 81 22
BspHI T'CATG_A
Cuts at: 0 860 1752
Size: 860 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1077 1752
Size: 1077 675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 268 1752
Size: 268 1484
BstEII G'GTnAC_C
Cuts at: 0 4 1752
Size: 4 1748
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1006 1752
Size: 1006 746
BstYI r'GATC_y
Cuts at: 0 504 1752
Size: 504 1248
BtgI C'CryG_G
Cuts at: 0 1586 1752
Size: 1586 166
BtsI GCAGTG_nn'
Cuts at: 0 1603 1752
Size: 1603 149
DraI TTT'AAA
Cuts at: 0 873 1671 1752
Size: 873 798 81
Fragments arranged by size:
873 798 81
EaeI y'GGCC_r
Cuts at: 0 176 1752
Size: 176 1576
EarI CTCTTCn'nnn_
Cuts at: 0 1531 1752
Size: 1531 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 555 823 935 1389 1752
Size: 368 187 268 112 454 363
Fragments arranged by size:
454 368 363 268 187 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 308 527 1752
Size: 308 219 1225
Fragments arranged by size:
1225 308 219
EcoO109I rG'GnC_Cy
Cuts at: 0 486 789 912 1099 1752
Size: 486 303 123 187 653
Fragments arranged by size:
653 486 303 187 123
EcoRI G'AATT_C
Cuts at: 0 1014 1752
Size: 1014 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 282 314 355 387 949 981
Size: 64 32 186 32 41 32 562 32
Cuts at: 981 1495 1527 1585 1617 1708 1740 1752
Size: 514 32 58 32 91 32 12
Fragments arranged by size:
562 514 186 91 64 58 41 32
32 32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 0 113 144 732 1752
Size: 113 31 588 1020
Fragments arranged by size:
1020 588 113 31
HindIII A'AGCT_T
Cuts at: 0 358 1752
Size: 358 1394
MluI A'CGCG_T
Cuts at: 0 77 1752
Size: 77 1675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 1752
Size: 1676 76
MscI TGG'CCA
Cuts at: 0 178 1752
Size: 178 1574
MspA1I CmG'CkG
Cuts at: 0 268 430 955 1409 1752
Size: 268 162 525 454 343
Fragments arranged by size:
525 454 343 268 162
PasI CC'CwG_GG
Cuts at: 0 460 784 1752
Size: 460 324 968
Fragments arranged by size:
968 460 324
PpuMI rG'GwC_Cy
Cuts at: 0 486 789 912 1099 1752
Size: 486 303 123 187 653
Fragments arranged by size:
653 486 303 187 123
PshAI GACnn'nnGTC
Cuts at: 0 1347 1752
Size: 1347 405
PsiI TTA'TAA
Cuts at: 0 1210 1752
Size: 1210 542
PstI C_TGCA'G
Cuts at: 0 717 1610 1752
Size: 717 893 142
Fragments arranged by size:
893 717 142
PvuII CAG'CTG
Cuts at: 0 268 430 955 1409 1752
Size: 268 162 525 454 343
Fragments arranged by size:
525 454 343 268 162
SalI G'TCGA_C
Cuts at: 0 111 142 1752
Size: 111 31 1610
Fragments arranged by size:
1610 111 31
SanDI GG'GwC_CC
Cuts at: 0 789 1752
Size: 789 963
SapI GCTCTTCn'nnn_
Cuts at: 0 1531 1752
Size: 1531 221
SbfI CC_TGCA'GG
Cuts at: 0 717 1752
Size: 717 1035
SfcI C'TryA_G
Cuts at: 0 713 836 1606 1752
Size: 713 123 770 146
Fragments arranged by size:
770 713 146 123
SmlI C'TyrA_G
Cuts at: 0 961 1703 1752
Size: 961 742 49
Fragments arranged by size:
961 742 49
StuI AGG'CCT
Cuts at: 0 1676 1752
Size: 1676 76
StyI C'CwwG_G
Cuts at: 0 345 563 999 1085 1217 1656 1752
Size: 345 218 436 86 132 439 96
Fragments arranged by size:
439 436 345 218 132 96 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 957 1390 1752
Size: 957 433 362
Fragments arranged by size:
957 433 362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1752
Size: 1033 32 687
Fragments arranged by size:
1033 687 32
Tth111I GACn'n_nGTC
Cuts at: 0 484 1156 1752
Size: 484 672 596
Fragments arranged by size:
672 596 484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 407 1752
Size: 407 1345
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BcgI BciVI BglI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaWI BseRI
BsgI BsiHKAI Bsp1286I BspHI BspMI BstAPI BstEII BstXI
BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HindIII MluI MmeI MscI
MspA1I PasI PpuMI PshAI PsiI PstI PvuII SalI
SanDI SapI SbfI SfcI SmlI StuI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI
ZraI