Restriction map for
pFN21AE5630
(Linear) (Six-base) MAPSORT of: KIEE5630.seq Check: 2023 from: 1 to: 1542
KIEE5630.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1217 1542
Size: 1217 325
Acc65I G'GTAC_C
Cuts at: 0 647 1542
Size: 647 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 555 637 764 1396 1542
Size: 205 350 82 127 632 146
Fragments arranged by size:
632 350 205 146 127 82
AleI CACnn'nnGTG
Cuts at: 0 457 784 1542
Size: 457 327 758
Fragments arranged by size:
758 457 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1542
Size: 1012 32 498
Fragments arranged by size:
1012 498 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 66 351 987 1182 1425 1542
Size: 18 48 285 636 195 243 117
Fragments arranged by size:
636 285 243 195 117 48 18
ApoI r'AATT_y
Cuts at: 0 555 588 756 1429 1542
Size: 555 33 168 673 113
Fragments arranged by size:
673 555 168 113 33
AvaI C'yCGr_G
Cuts at: 0 361 367 1542
Size: 361 6 1175
Fragments arranged by size:
1175 361 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 631 664 1542
Size: 631 33 878
Fragments arranged by size:
878 631 33
BamHI G'GATC_C
Cuts at: 0 912 1542
Size: 912 630
BanI G'GyrC_C
Cuts at: 0 647 1542
Size: 647 895
BanII G_rGCy'C
Cuts at: 0 205 368 443 477 1124 1542
Size: 205 163 75 34 647 418
Fragments arranged by size:
647 418 205 163 75 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 755 1299 1397 1542
Size: 402 353 544 98 145
Fragments arranged by size:
544 402 353 145 98
BclI T'GATC_A
Cuts at: 0 530 1542
Size: 530 1012
Bme1580I G_kGCm'C
Cuts at: 0 1053 1542
Size: 1053 489
BmrI ACTGGGnnnn_n'
Cuts at: 0 975 1542
Size: 975 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 823 1014 1300 1542
Size: 21 802 191 286 242
Fragments arranged by size:
802 286 242 191 21
Bpu10I CC'TnA_GC
Cuts at: 0 1236 1542
Size: 1236 306
BsaI GGTCTCn'nnnn_
Cuts at: 0 1276 1542
Size: 1276 266
BsaWI w'CCGG_w
Cuts at: 0 1148 1542
Size: 1148 394
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1012 1042 1542
Size: 1012 30 500
Fragments arranged by size:
1012 500 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 1542
Size: 136 1406
BseYI C'CCAG_C
Cuts at: 0 500 1542
Size: 500 1042
BsiEI CG_ry'CG
Cuts at: 0 920 1542
Size: 920 622
BsiHKAI G_wGCw'C
Cuts at: 0 235 1234 1542
Size: 235 999 308
Fragments arranged by size:
999 308 235
Bsp1286I G_dGCh'C
Cuts at: 0 205 235 368 443 477 1053 1124 1234
Size: 205 30 133 75 34 576 71 110
Cuts at: 1234 1542
Size: 308
Fragments arranged by size:
576 308 205 133 110 75 71 34
30
BspEI T'CCGG_A
Cuts at: 0 1148 1542
Size: 1148 394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 803 1217 1542
Size: 359 444 414 325
Fragments arranged by size:
444 414 359 325
BssSI C'ACGA_G
Cuts at: 0 1052 1542
Size: 1052 490
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1229 1542
Size: 1229 313
BstEII G'GTnAC_C
Cuts at: 0 450 1084 1542
Size: 450 634 458
Fragments arranged by size:
634 458 450
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 789 909 1198 1542
Size: 243 546 120 289 344
Fragments arranged by size:
546 344 289 243 120
BstYI r'GATC_y
Cuts at: 0 912 1250 1298 1469 1542
Size: 912 338 48 171 73
Fragments arranged by size:
912 338 171 73 48
Bsu36I CC'TnA_GG
Cuts at: 0 376 512 1542
Size: 376 136 1030
Fragments arranged by size:
1030 376 136
BtgI C'CryG_G
Cuts at: 0 116 1542
Size: 116 1426
BtsI GCAGTG_nn'
Cuts at: 0 1209 1542
Size: 1209 333
EaeI y'GGCC_r
Cuts at: 0 604 917 1542
Size: 604 313 625
Fragments arranged by size:
625 604 313
EagI C'GGCC_G
Cuts at: 0 917 1542
Size: 917 625
EarI CTCTTCn'nnn_
Cuts at: 0 86 494 613 1039 1542
Size: 86 408 119 426 503
Fragments arranged by size:
503 426 408 119 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 205 555 637 764 823 1014 1300
Size: 21 184 350 82 127 59 191 286
Cuts at: 1300 1396 1542
Size: 96 146
Fragments arranged by size:
350 286 191 184 146 127 96 82
59 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 991 1542
Size: 532 459 551
Fragments arranged by size:
551 532 459
EcoO109I rG'GnC_Cy
Cuts at: 0 348 372 481 671 974 1542
Size: 348 24 109 190 303 568
Fragments arranged by size:
568 348 303 190 109 24
EcoRI G'AATT_C
Cuts at: 0 1429 1542
Size: 1429 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 944 976 1542
Size: 944 32 566
Fragments arranged by size:
944 566 32
KpnI G_GTAC'C
Cuts at: 0 651 1542
Size: 651 891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1542
Size: 746 796
MslI CAynn'nnrTG
Cuts at: 0 305 457 784 787 1542
Size: 305 152 327 3 755
Fragments arranged by size:
755 327 305 152 3
MspA1I CmG'CkG
Cuts at: 0 1034 1425 1542
Size: 1034 391 117
Fragments arranged by size:
1034 391 117
PasI CC'CwG_GG
Cuts at: 0 477 1542
Size: 477 1065
PfoI T'CCnGG_A
Cuts at: 0 281 549 800 908 1542
Size: 281 268 251 108 634
Fragments arranged by size:
634 281 268 251 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1542
Size: 1012 32 498
Fragments arranged by size:
1012 498 32
PpuMI rG'GwC_Cy
Cuts at: 0 348 372 671 1542
Size: 348 24 299 871
Fragments arranged by size:
871 348 299 24
PspXI vC'TCGA_Gb
Cuts at: 0 367 1542
Size: 367 1175
PstI C_TGCA'G
Cuts at: 0 71 584 1178 1451 1542
Size: 71 513 594 273 91
Fragments arranged by size:
594 513 273 91 71
PvuII CAG'CTG
Cuts at: 0 1034 1425 1542
Size: 1034 391 117
Fragments arranged by size:
1034 391 117
RsrII CG'GwC_CG
Cuts at: 0 113 1542
Size: 113 1429
SapI GCTCTTCn'nnn_
Cuts at: 0 613 1542
Size: 613 929
SbfI CC_TGCA'GG
Cuts at: 0 1451 1542
Size: 1451 91
ScaI AGT'ACT
Cuts at: 0 838 1542
Size: 838 704
SfcI C'TryA_G
Cuts at: 0 67 468 580 1174 1447 1542
Size: 67 401 112 594 273 95
Fragments arranged by size:
594 401 273 112 95 67
SmlI C'TyrA_G
Cuts at: 0 367 1542
Size: 367 1175
StuI AGG'CCT
Cuts at: 0 538 1542
Size: 538 1004
StyI C'CwwG_G
Cuts at: 0 1434 1542
Size: 1434 108
TatI w'GTAC_w
Cuts at: 0 836 1542
Size: 836 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1043 1075 1542
Size: 1043 32 467
Fragments arranged by size:
1043 467 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 933 1542
Size: 154 779 609
Fragments arranged by size:
779 609 154
XhoI C'TCGA_G
Cuts at: 0 367 1542
Size: 367 1175
Enzymes that do cut:
AarI Acc65I AcuI AleI AloI AlwNI ApoI AvaI
BaeI BamHI BanI BanII BbsI BclI Bme1580I BmrI
BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MslI MspA1I
PasI PfoI PpiI PpuMI PspXI PstI PvuII RsrII
SapI SbfI ScaI SfcI SmlI StuI StyI TatI
TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsgI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspOMI PsrI PvuI SacI SacII SalI SanDI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XmaI XmnI
ZraI