Restriction map for
pFN21AE5625
(Linear) (Six-base) MAPSORT of: KIEE5625.seq Check: 4382 from: 1 to: 1479
KIEE5625.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AccI GT'mk_AC
Cuts at: 0 1234 1479
Size: 1234 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 998 1058 1479
Size: 254 744 60 421
Fragments arranged by size:
744 421 254 60
AlwNI CAG_nnn'CTG
Cuts at: 0 400 978 1479
Size: 400 578 501
Fragments arranged by size:
578 501 400
ApaI G_GGCC'C
Cuts at: 0 1387 1479
Size: 1387 92
ApoI r'AATT_y
Cuts at: 0 926 1479
Size: 926 553
AvaI C'yCGr_G
Cuts at: 0 725 920 1479
Size: 725 195 559
Fragments arranged by size:
725 559 195
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1210 1243 1479
Size: 1210 33 236
Fragments arranged by size:
1210 236 33
BanI G'GyrC_C
Cuts at: 0 949 1130 1479
Size: 949 181 349
Fragments arranged by size:
949 349 181
BanII G_rGCy'C
Cuts at: 0 288 1336 1387 1395 1479
Size: 288 1048 51 8 84
Fragments arranged by size:
1048 288 84 51 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 246 908 1479
Size: 210 36 662 571
Fragments arranged by size:
662 571 210 36
BbvCI CC'TCA_GC
Cuts at: 0 421 572 1479
Size: 421 151 907
Fragments arranged by size:
907 421 151
BglI GCCn_nnn'nGGC
Cuts at: 0 991 1479
Size: 991 488
BlpI GC'TnA_GC
Cuts at: 0 178 1479
Size: 178 1301
Bme1580I G_kGCm'C
Cuts at: 0 1387 1479
Size: 1387 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 906 1479
Size: 906 573
BmtI G_CTAG'C
Cuts at: 0 1405 1479
Size: 1405 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1379 1479
Size: 1379 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 898 1479
Size: 799 99 581
Fragments arranged by size:
799 581 99
Bpu10I CC'TnA_GC
Cuts at: 0 421 496 572 1394 1479
Size: 421 75 76 822 85
Fragments arranged by size:
822 421 85 76 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 742 1479
Size: 124 618 737
Fragments arranged by size:
737 618 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 958 1479
Size: 958 521
BsaBI GATnn'nnATC
Cuts at: 0 469 1479
Size: 469 1010
BsaWI w'CCGG_w
Cuts at: 0 1079 1479
Size: 1079 400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 577 796 976 1188 1342 1479
Size: 139 438 219 180 212 154 137
Fragments arranged by size:
438 219 212 180 154 139 137
BseYI C'CCAG_C
Cuts at: 0 986 1092 1320 1479
Size: 986 106 228 159
Fragments arranged by size:
986 228 159 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1479
Size: 835 644
BsiHKAI G_wGCw'C
Cuts at: 0 179 1395 1479
Size: 179 1216 84
Fragments arranged by size:
1216 179 84
Bsp1286I G_dGCh'C
Cuts at: 0 179 288 1336 1387 1395 1479
Size: 179 109 1048 51 8 84
Fragments arranged by size:
1048 179 109 84 51 8
BspHI T'CATG_A
Cuts at: 0 651 1479
Size: 651 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 466 1479
Size: 466 1013
BsrBI CCG'CTC
Cuts at: 0 106 1479
Size: 106 1373
BsrDI GCAATG_nn'
Cuts at: 0 527 605 1479
Size: 527 78 874
Fragments arranged by size:
874 527 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1260 1378 1479
Size: 1260 118 101
Fragments arranged by size:
1260 118 101
BstYI r'GATC_y
Cuts at: 0 416 646 1416 1479
Size: 416 230 770 63
Fragments arranged by size:
770 416 230 63
Bsu36I CC'TnA_GG
Cuts at: 0 1310 1479
Size: 1310 169
BtgI C'CryG_G
Cuts at: 0 698 1479
Size: 698 781
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 464 522 1479
Size: 464 58 957
Fragments arranged by size:
957 464 58
EaeI y'GGCC_r
Cuts at: 0 296 1479
Size: 296 1183
EarI CTCTTCn'nnn_
Cuts at: 0 77 714 1479
Size: 77 637 765
Fragments arranged by size:
765 637 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1279 1479
Size: 1279 200
EcoICRI GAG'CTC
Cuts at: 0 1393 1479
Size: 1393 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 799 898 998 1058 1479
Size: 254 545 99 100 60 421
Fragments arranged by size:
545 421 254 100 99 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 781 1309 1479
Size: 760 21 528 170
Fragments arranged by size:
760 528 170 21
EcoO109I rG'GnC_Cy
Cuts at: 0 1302 1383 1384 1479
Size: 1302 81 1 95
Fragments arranged by size:
1302 95 81 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 867 1449 1479
Size: 867 582 30
Fragments arranged by size:
867 582 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 1479
Size: 604 32 843
Fragments arranged by size:
843 604 32
HincII GTy'rAC
Cuts at: 0 336 1479
Size: 336 1143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1479
Size: 687 792
MscI TGG'CCA
Cuts at: 0 298 1479
Size: 298 1181
MslI CAynn'nnrTG
Cuts at: 0 1060 1479
Size: 1060 419
MspA1I CmG'CkG
Cuts at: 0 587 876 1036 1479
Size: 587 289 160 443
Fragments arranged by size:
587 443 289 160
NheI G'CTAG_C
Cuts at: 0 1401 1479
Size: 1401 78
PasI CC'CwG_GG
Cuts at: 0 888 1378 1479
Size: 888 490 101
Fragments arranged by size:
888 490 101
PspOMI G'GGCC_C
Cuts at: 0 1383 1479
Size: 1383 96
PstI C_TGCA'G
Cuts at: 0 1278 1479
Size: 1278 201
PvuII CAG'CTG
Cuts at: 0 587 876 1036 1479
Size: 587 289 160 443
Fragments arranged by size:
587 443 289 160
SacI G_AGCT'C
Cuts at: 0 1395 1479
Size: 1395 84
ScaI AGT'ACT
Cuts at: 0 847 1479
Size: 847 632
SfcI C'TryA_G
Cuts at: 0 1274 1479
Size: 1274 205
SmaI CCC'GGG
Cuts at: 0 922 1479
Size: 922 557
SmlI C'TyrA_G
Cuts at: 0 139 757 1479
Size: 139 618 722
Fragments arranged by size:
722 618 139
SspI AAT'ATT
Cuts at: 0 358 1479
Size: 358 1121
StuI AGG'CCT
Cuts at: 0 937 1479
Size: 937 542
StyI C'CwwG_G
Cuts at: 0 365 945 1442 1479
Size: 365 580 497 37
Fragments arranged by size:
580 497 365 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1312 1479
Size: 1312 167
TatI w'GTAC_w
Cuts at: 0 845 1479
Size: 845 634
Tth111I GACn'n_nGTC
Cuts at: 0 193 1479
Size: 193 1286
XbaI T'CTAG_A
Cuts at: 0 1182 1479
Size: 1182 297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 975 1479
Size: 372 603 504
Fragments arranged by size:
603 504 372
XmaI C'CCGG_G
Cuts at: 0 920 1479
Size: 920 559
XmnI GAAnn'nnTTC
Cuts at: 0 1169 1479
Size: 1169 310
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AvaI BaeI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MmeI
MscI MslI MspA1I NheI PasI PspOMI PstI PvuII
SacI ScaI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglII
BmgBI BsaAI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TstI XhoI ZraI