Restriction map for
pFN21AE5622
(Linear) (Six-base) MAPSORT of: KIEE5622.seq Check: 8182 from: 1 to: 1608
KIEE5622.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 1608
Size: 615 993
AccI GT'mk_AC
Cuts at: 0 320 1608
Size: 320 1288
AflIII A'CryG_T
Cuts at: 0 960 1608
Size: 960 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1551 1608
Size: 1551 57
AlwNI CAG_nnn'CTG
Cuts at: 0 250 1608
Size: 250 1358
BamHI G'GATC_C
Cuts at: 0 829 1608
Size: 829 779
BanI G'GyrC_C
Cuts at: 0 597 1608
Size: 597 1011
BbeI G_GCGC'C
Cuts at: 0 601 1608
Size: 601 1007
BclI T'GATC_A
Cuts at: 0 102 687 1301 1608
Size: 102 585 614 307
Fragments arranged by size:
614 585 307 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 650 1608
Size: 489 161 958
Fragments arranged by size:
958 489 161
Bpu10I CC'TnA_GC
Cuts at: 0 876 1038 1588 1608
Size: 876 162 550 20
Fragments arranged by size:
876 550 162 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1608
Size: 1020 588
BsaI GGTCTCn'nnnn_
Cuts at: 0 626 1171 1608
Size: 626 545 437
Fragments arranged by size:
626 545 437
BsaBI GATnn'nnATC
Cuts at: 0 920 1608
Size: 920 688
BsaHI Gr'CG_yC
Cuts at: 0 598 1608
Size: 598 1010
BsaWI w'CCGG_w
Cuts at: 0 1118 1608
Size: 1118 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1608
Size: 487 30 1091
Fragments arranged by size:
1091 487 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 958 1159 1608
Size: 958 201 449
Fragments arranged by size:
958 449 201
BseYI C'CCAG_C
Cuts at: 0 499 1313 1608
Size: 499 814 295
Fragments arranged by size:
814 499 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1608
Size: 587 1021
BsiHKAI G_wGCw'C
Cuts at: 0 550 1608
Size: 550 1058
Bsp1286I G_dGCh'C
Cuts at: 0 550 1608
Size: 550 1058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 1608
Size: 615 993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 343 847 1317 1579 1608
Size: 343 504 470 262 29
Fragments arranged by size:
504 470 343 262 29
BstYI r'GATC_y
Cuts at: 0 829 1608
Size: 829 779
Bsu36I CC'TnA_GG
Cuts at: 0 326 1608
Size: 326 1282
BtgI C'CryG_G
Cuts at: 0 1056 1608
Size: 1056 552
BtsI GCAGTG_nn'
Cuts at: 0 1192 1608
Size: 1192 416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1143 1178 1608
Size: 1143 35 430
Fragments arranged by size:
1143 430 35
EaeI y'GGCC_r
Cuts at: 0 990 1331 1608
Size: 990 341 277
Fragments arranged by size:
990 341 277
EarI CTCTTCn'nnn_
Cuts at: 0 2 1570 1608
Size: 2 1568 38
Fragments arranged by size:
1568 38 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 650 1608
Size: 489 161 958
Fragments arranged by size:
958 489 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1333 1608
Size: 1333 275
EcoO109I rG'GnC_Cy
Cuts at: 0 855 1608
Size: 855 753
EcoRV GAT'ATC
Cuts at: 0 386 1608
Size: 386 1222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 146 668 1608
Size: 146 522 940
Fragments arranged by size:
940 522 146
HaeII r_GCGC'y
Cuts at: 0 601 1608
Size: 601 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 676 708 1608
Size: 187 32 457 32 900
Fragments arranged by size:
900 457 187 32 32
KasI G'GCGC_C
Cuts at: 0 597 1608
Size: 597 1011
MfeI C'AATT_G
Cuts at: 0 401 1608
Size: 401 1207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1608
Size: 557 1051
MscI TGG'CCA
Cuts at: 0 992 1333 1608
Size: 992 341 275
Fragments arranged by size:
992 341 275
MspA1I CmG'CkG
Cuts at: 0 1317 1608
Size: 1317 291
NarI GG'CG_CC
Cuts at: 0 598 1608
Size: 598 1010
NcoI C'CATG_G
Cuts at: 0 1056 1608
Size: 1056 552
NdeI CA'TA_TG
Cuts at: 0 1455 1608
Size: 1455 153
NspI r_CATG'y
Cuts at: 0 167 964 1608
Size: 167 797 644
Fragments arranged by size:
797 644 167
PciI A'CATG_T
Cuts at: 0 960 1608
Size: 960 648
PfoI T'CCnGG_A
Cuts at: 0 694 1608
Size: 694 914
PpuMI rG'GwC_Cy
Cuts at: 0 855 1608
Size: 855 753
PstI C_TGCA'G
Cuts at: 0 612 1199 1608
Size: 612 587 409
Fragments arranged by size:
612 587 409
PvuII CAG'CTG
Cuts at: 0 1317 1608
Size: 1317 291
SapI GCTCTTCn'nnn_
Cuts at: 0 2 1608
Size: 2 1606
SexAI A'CCwGG_T
Cuts at: 0 953 1608
Size: 953 655
SfcI C'TryA_G
Cuts at: 0 295 608 1008 1195 1383 1608
Size: 295 313 400 187 188 225
Fragments arranged by size:
400 313 295 225 188 187
SfoI GGC'GCC
Cuts at: 0 599 1608
Size: 599 1009
SmlI C'TyrA_G
Cuts at: 0 999 1608
Size: 999 609
SspI AAT'ATT
Cuts at: 0 411 1364 1608
Size: 411 953 244
Fragments arranged by size:
953 411 244
StuI AGG'CCT
Cuts at: 0 490 1608
Size: 490 1118
StyI C'CwwG_G
Cuts at: 0 1056 1334 1608
Size: 1056 278 274
Fragments arranged by size:
1056 278 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1060 1150 1327 1608
Size: 1060 90 177 281
Fragments arranged by size:
1060 281 177 90
XmnI GAAnn'nnTTC
Cuts at: 0 1364 1608
Size: 1364 244
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI BamHI BanI BbeI
BclI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MfeI
MmeI MscI MspA1I NarI NcoI NdeI NspI PciI
PfoI PpuMI PstI PvuII SapI SexAI SfcI SfoI
SmlI SspI StuI StyI TaqII XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaAI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI FseI FspI FspAI HincII HindIII
HpaI KpnI MluI MslI NaeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI