Restriction map for
pFN21AE5621
(Linear) (Six-base) MAPSORT of: KIEE5621.seq Check: 5533 from: 1 to: 1536
KIEE5621.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1536
Size: 511 1025
AflIII A'CryG_T
Cuts at: 0 581 1536
Size: 581 955
AleI CACnn'nnGTG
Cuts at: 0 223 553 1536
Size: 223 330 983
Fragments arranged by size:
983 330 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 1536
Size: 628 908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 1536
Size: 719 32 785
Fragments arranged by size:
785 719 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1536
Size: 1033 503
ApoI r'AATT_y
Cuts at: 0 1168 1536
Size: 1168 368
AvrII C'CTAG_G
Cuts at: 0 1038 1536
Size: 1038 498
BanII G_rGCy'C
Cuts at: 0 154 181 1118 1349 1536
Size: 154 27 937 231 187
Fragments arranged by size:
937 231 187 154 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 246 1536
Size: 246 1290
BciVI GTATCCnnnnn_n'
Cuts at: 0 1339 1536
Size: 1339 197
BclI T'GATC_A
Cuts at: 0 1370 1536
Size: 1370 166
BglII A'GATC_T
Cuts at: 0 53 1536
Size: 53 1483
Bme1580I G_kGCm'C
Cuts at: 0 1496 1536
Size: 1496 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 1536
Size: 14 1522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 421 481 670 779 1536
Size: 282 139 60 189 109 757
Fragments arranged by size:
757 282 189 139 109 60
Bpu10I CC'TnA_GC
Cuts at: 0 1007 1536
Size: 1007 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1536
Size: 1180 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 928 1536
Size: 928 608
BsaWI w'CCGG_w
Cuts at: 0 290 1536
Size: 290 1246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 719 749 1536
Size: 187 30 502 30 787
Fragments arranged by size:
787 502 187 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1420 1536
Size: 1420 116
BseYI C'CCAG_C
Cuts at: 0 258 328 777 1135 1536
Size: 258 70 449 358 401
Fragments arranged by size:
449 401 358 258 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1046 1536
Size: 454 592 490
Fragments arranged by size:
592 490 454
BsiHKAI G_wGCw'C
Cuts at: 0 181 1118 1349 1536
Size: 181 937 231 187
Fragments arranged by size:
937 231 187 181
Bsp1286I G_dGCh'C
Cuts at: 0 154 181 1118 1349 1496 1536
Size: 154 27 937 231 147 40
Fragments arranged by size:
937 231 154 147 40 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 692 851 1400 1536
Size: 368 324 159 549 136
Fragments arranged by size:
549 368 324 159 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1033 1536
Size: 1033 503
BstYI r'GATC_y
Cuts at: 0 53 365 740 1130 1536
Size: 53 312 375 390 406
Fragments arranged by size:
406 390 375 312 53
BtgI C'CryG_G
Cuts at: 0 78 620 853 1121 1536
Size: 78 542 233 268 415
Fragments arranged by size:
542 415 268 233 78
BtsI GCAGTG_nn'
Cuts at: 0 143 1536
Size: 143 1393
DraIII CAC_nnn'GTG
Cuts at: 0 477 1485 1536
Size: 477 1008 51
Fragments arranged by size:
1008 477 51
EaeI y'GGCC_r
Cuts at: 0 534 815 1536
Size: 534 281 721
Fragments arranged by size:
721 534 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 1066 1536
Size: 76 990 470
Fragments arranged by size:
990 470 76
EcoICRI GAG'CTC
Cuts at: 0 179 1116 1347 1536
Size: 179 937 231 189
Fragments arranged by size:
937 231 189 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 421 481 511 670 779 1536
Size: 282 139 60 30 159 109 757
Fragments arranged by size:
757 282 159 139 109 60 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1037 1536
Size: 1037 499
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1514 1536
Size: 405 1109 22
Fragments arranged by size:
1109 405 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 1536
Size: 441 1095
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 732 764 853 885 1536
Size: 175 32 525 32 89 32 651
Fragments arranged by size:
651 525 175 89 32 32 32
HincII GTy'rAC
Cuts at: 0 357 1536
Size: 357 1179
MfeI C'AATT_G
Cuts at: 0 1247 1536
Size: 1247 289
MscI TGG'CCA
Cuts at: 0 817 1536
Size: 817 719
MslI CAynn'nnrTG
Cuts at: 0 97 223 553 1536
Size: 97 126 330 983
Fragments arranged by size:
983 330 126 97
MspA1I CmG'CkG
Cuts at: 0 111 262 1536
Size: 111 151 1274
Fragments arranged by size:
1274 151 111
NcoI C'CATG_G
Cuts at: 0 78 1121 1536
Size: 78 1043 415
Fragments arranged by size:
1043 415 78
NspI r_CATG'y
Cuts at: 0 217 459 585 981 1536
Size: 217 242 126 396 555
Fragments arranged by size:
555 396 242 217 126
PasI CC'CwG_GG
Cuts at: 0 1509 1536
Size: 1509 27
PciI A'CATG_T
Cuts at: 0 581 1536
Size: 581 955
PflMI CCAn_nnn'nTGG
Cuts at: 0 777 1536
Size: 777 759
PfoI T'CCnGG_A
Cuts at: 0 368 1536
Size: 368 1168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 719 751 1536
Size: 719 32 785
Fragments arranged by size:
785 719 32
PpuMI rG'GwC_Cy
Cuts at: 0 1514 1536
Size: 1514 22
PstI C_TGCA'G
Cuts at: 0 365 1081 1502 1536
Size: 365 716 421 34
Fragments arranged by size:
716 421 365 34
PvuII CAG'CTG
Cuts at: 0 262 1536
Size: 262 1274
SacI G_AGCT'C
Cuts at: 0 181 1118 1349 1536
Size: 181 937 231 187
Fragments arranged by size:
937 231 187 181
ScaI AGT'ACT
Cuts at: 0 1440 1536
Size: 1440 96
SfcI C'TryA_G
Cuts at: 0 361 918 1077 1498 1536
Size: 361 557 159 421 38
Fragments arranged by size:
557 421 361 159 38
SmlI C'TyrA_G
Cuts at: 0 7 1159 1536
Size: 7 1152 377
Fragments arranged by size:
1152 377 7
SphI G_CATG'C
Cuts at: 0 459 1536
Size: 459 1077
StyI C'CwwG_G
Cuts at: 0 78 1038 1121 1517 1536
Size: 78 960 83 396 19
Fragments arranged by size:
960 396 83 78 19
TatI w'GTAC_w
Cuts at: 0 1438 1536
Size: 1438 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 752 784 915 947 1536
Size: 752 32 131 32 589
Fragments arranged by size:
752 589 131 32 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApoI AvrII
BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BstAPI BstYI BtgI BtsI DraIII
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII MfeI MscI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpiI PpuMI PstI PvuII SacI
ScaI SfcI SmlI SphI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EarI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI