Restriction map for
pFN21AE5619
(Linear) (Six-base) MAPSORT of: KIEE5619.seq Check: 2043 from: 1 to: 1443
KIEE5619.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 534 1443
Size: 485 49 909
Fragments arranged by size:
909 485 49
AflIII A'CryG_T
Cuts at: 0 189 1079 1443
Size: 189 890 364
Fragments arranged by size:
890 364 189
AleI CACnn'nnGTG
Cuts at: 0 338 1443
Size: 338 1105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1214 1443
Size: 1214 229
AlwNI CAG_nnn'CTG
Cuts at: 0 142 1443
Size: 142 1301
ApaI G_GGCC'C
Cuts at: 0 534 1443
Size: 534 909
ApoI r'AATT_y
Cuts at: 0 129 955 1443
Size: 129 826 488
Fragments arranged by size:
826 488 129
AseI AT'TA_AT
Cuts at: 0 704 1443
Size: 704 739
BamHI G'GATC_C
Cuts at: 0 1277 1443
Size: 1277 166
BanI G'GyrC_C
Cuts at: 0 652 1443
Size: 652 791
BanII G_rGCy'C
Cuts at: 0 534 757 1443
Size: 534 223 686
Fragments arranged by size:
686 534 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 192 1382 1443
Size: 192 1190 61
Fragments arranged by size:
1190 192 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 217 1443
Size: 217 1226
Bme1580I G_kGCm'C
Cuts at: 0 534 1443
Size: 534 909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 491 1443
Size: 82 409 952
Fragments arranged by size:
952 409 82
Bpu10I CC'TnA_GC
Cuts at: 0 1019 1443
Size: 1019 424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 716 1443
Size: 592 124 727
Fragments arranged by size:
727 592 124
BsaAI yAC'GTr
Cuts at: 0 192 1294 1443
Size: 192 1102 149
Fragments arranged by size:
1102 192 149
BsaBI GATnn'nnATC
Cuts at: 0 850 1443
Size: 850 593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 1443
Size: 460 983
BseYI C'CCAG_C
Cuts at: 0 218 1443
Size: 218 1225
BsiHKAI G_wGCw'C
Cuts at: 0 177 1313 1443
Size: 177 1136 130
Fragments arranged by size:
1136 177 130
BsmI GAATG_Cn'
Cuts at: 0 817 1443
Size: 817 626
Bsp1286I G_dGCh'C
Cuts at: 0 177 534 757 1313 1443
Size: 177 357 223 556 130
Fragments arranged by size:
556 357 223 177 130
BsrBI CCG'CTC
Cuts at: 0 576 1443
Size: 576 867
BssSI C'ACGA_G
Cuts at: 0 599 1443
Size: 599 844
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 1443
Size: 72 1371
BstYI r'GATC_y
Cuts at: 0 470 1001 1277 1443
Size: 470 531 276 166
Fragments arranged by size:
531 470 276 166
BtgI C'CryG_G
Cuts at: 0 623 1443
Size: 623 820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1033 1068 1443
Size: 1033 35 375
Fragments arranged by size:
1033 375 35
EaeI y'GGCC_r
Cuts at: 0 180 810 867 1443
Size: 180 630 57 576
Fragments arranged by size:
630 576 180 57
EarI CTCTTCn'nnn_
Cuts at: 0 263 461 1399 1443
Size: 263 198 938 44
Fragments arranged by size:
938 263 198 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 485 491 534 1443
Size: 82 403 6 43 909
Fragments arranged by size:
909 403 82 43 6
EcoO109I rG'GnC_Cy
Cuts at: 0 531 1443
Size: 531 912
FspI TGC'GCA
Cuts at: 0 1225 1443
Size: 1225 218
FspAI rTGC'GCAy
Cuts at: 0 1225 1443
Size: 1225 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 365 382 397 414 1443
Size: 365 17 15 17 1029
Fragments arranged by size:
1029 365 17 17 15
HincII GTy'rAC
Cuts at: 0 1419 1443
Size: 1419 24
HindIII A'AGCT_T
Cuts at: 0 546 1443
Size: 546 897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1443
Size: 350 1093
MscI TGG'CCA
Cuts at: 0 812 869 1443
Size: 812 57 574
Fragments arranged by size:
812 574 57
MslI CAynn'nnrTG
Cuts at: 0 338 1443
Size: 338 1105
MspA1I CmG'CkG
Cuts at: 0 566 1443
Size: 566 877
NcoI C'CATG_G
Cuts at: 0 623 1443
Size: 623 820
NspI r_CATG'y
Cuts at: 0 1083 1443
Size: 1083 360
PciI A'CATG_T
Cuts at: 0 1079 1443
Size: 1079 364
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 1266 1443
Size: 876 390 177
Fragments arranged by size:
876 390 177
PfoI T'CCnGG_A
Cuts at: 0 1273 1443
Size: 1273 170
PspOMI G'GGCC_C
Cuts at: 0 530 1443
Size: 530 913
PvuII CAG'CTG
Cuts at: 0 566 1443
Size: 566 877
SfcI C'TryA_G
Cuts at: 0 536 950 1443
Size: 536 414 493
Fragments arranged by size:
536 493 414
SmlI C'TyrA_G
Cuts at: 0 571 731 1443
Size: 571 160 712
Fragments arranged by size:
712 571 160
SnaBI TAC'GTA
Cuts at: 0 1294 1443
Size: 1294 149
SpeI A'CTAG_T
Cuts at: 0 303 1443
Size: 303 1140
SspI AAT'ATT
Cuts at: 0 1032 1443
Size: 1032 411
StyI C'CwwG_G
Cuts at: 0 409 623 870 1260 1443
Size: 409 214 247 390 183
Fragments arranged by size:
409 390 247 214 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 670 1328 1443
Size: 670 658 115
Fragments arranged by size:
670 658 115
TatI w'GTAC_w
Cuts at: 0 735 1443
Size: 735 708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1307 1339 1443
Size: 1307 32 104
Fragments arranged by size:
1307 104 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApoI AseI
BamHI BanI BanII BbsI BciVI Bme1580I BpmI Bpu10I
BpuEI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I
BsrBI BssSI BstAPI BstYI BtgI CspCI EaeI EarI
Eco57MI EcoO109I FspI FspAI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PfoI
PspOMI PvuII SfcI SmlI SnaBI SpeI SspI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BsaI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI