Restriction map for
pFN21AE5617
(Linear) (Six-base) MAPSORT of: KIEE5617.seq Check: 5061 from: 1 to: 1638
KIEE5617.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AccI GT'mk_AC
Cuts at: 0 173 1638
Size: 173 1465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 710 923 1613 1638
Size: 341 369 213 690 25
Fragments arranged by size:
690 369 341 213 25
AflIII A'CryG_T
Cuts at: 0 1082 1638
Size: 1082 556
AlwNI CAG_nnn'CTG
Cuts at: 0 574 733 1449 1638
Size: 574 159 716 189
Fragments arranged by size:
716 574 189 159
ApaI G_GGCC'C
Cuts at: 0 502 1638
Size: 502 1136
ApoI r'AATT_y
Cuts at: 0 1300 1638
Size: 1300 338
AvaI C'yCGr_G
Cuts at: 0 746 1638
Size: 746 892
BanI G'GyrC_C
Cuts at: 0 4 55 939 1452 1638
Size: 4 51 884 513 186
Fragments arranged by size:
884 513 186 51 4
BanII G_rGCy'C
Cuts at: 0 502 1538 1638
Size: 502 1036 100
Fragments arranged by size:
1036 502 100
BbeI G_GCGC'C
Cuts at: 0 59 1638
Size: 59 1579
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 1638
Size: 821 817
BclI T'GATC_A
Cuts at: 0 1092 1638
Size: 1092 546
BglI GCCn_nnn'nGGC
Cuts at: 0 295 1638
Size: 295 1343
Bme1580I G_kGCm'C
Cuts at: 0 502 1638
Size: 502 1136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 987 1593 1638
Size: 987 606 45
Fragments arranged by size:
987 606 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1173 1522 1638
Size: 539 634 349 116
Fragments arranged by size:
634 539 349 116
Bpu10I CC'TnA_GC
Cuts at: 0 140 1638
Size: 140 1498
BsaHI Gr'CG_yC
Cuts at: 0 56 305 1638
Size: 56 249 1333
Fragments arranged by size:
1333 249 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1393 1423 1638
Size: 70 30 1293 30 215
Fragments arranged by size:
1293 215 70 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 874 910 976 1582 1638
Size: 433 441 36 66 606 56
Fragments arranged by size:
606 441 433 66 56 36
BseYI C'CCAG_C
Cuts at: 0 17 709 1638
Size: 17 692 929
Fragments arranged by size:
929 692 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1638
Size: 410 1228
BsiHKAI G_wGCw'C
Cuts at: 0 40 1538 1577 1638
Size: 40 1498 39 61
Fragments arranged by size:
1498 61 40 39
BsmI GAATG_Cn'
Cuts at: 0 692 832 1140 1638
Size: 692 140 308 498
Fragments arranged by size:
692 498 308 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 114 1638
Size: 114 1524
Bsp1286I G_dGCh'C
Cuts at: 0 40 502 1538 1577 1638
Size: 40 462 1036 39 61
Fragments arranged by size:
1036 462 61 40 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1238 1504 1638
Size: 1238 266 134
Fragments arranged by size:
1238 266 134
BsrDI GCAATG_nn'
Cuts at: 0 1310 1638
Size: 1310 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 349 1638
Size: 349 1289
BstEII G'GTnAC_C
Cuts at: 0 1149 1638
Size: 1149 489
Bsu36I CC'TnA_GG
Cuts at: 0 575 1238 1638
Size: 575 663 400
Fragments arranged by size:
663 575 400
BtgI C'CryG_G
Cuts at: 0 113 1154 1638
Size: 113 1041 484
Fragments arranged by size:
1041 484 113
BtsI GCAGTG_nn'
Cuts at: 0 427 861 1638
Size: 427 434 777
Fragments arranged by size:
777 434 427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 974 1009 1638
Size: 974 35 629
Fragments arranged by size:
974 629 35
EarI CTCTTCn'nnn_
Cuts at: 0 434 455 729 803 833 899 923 1424
Size: 434 21 274 74 30 66 24 501
Cuts at: 1424 1638
Size: 214
Fragments arranged by size:
501 434 274 214 74 66 30 24
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 814 1638
Size: 78 736 824
Fragments arranged by size:
824 736 78
EcoICRI GAG'CTC
Cuts at: 0 1536 1638
Size: 1536 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 539 710 923 1173 1522 1613 1638
Size: 341 198 171 213 250 349 91 25
Fragments arranged by size:
349 341 250 213 198 171 91 25
EcoO109I rG'GnC_Cy
Cuts at: 0 498 499 545 552 571 1176 1638
Size: 498 1 46 7 19 605 462
Fragments arranged by size:
605 498 462 46 19 7 1
EcoRI G'AATT_C
Cuts at: 0 1300 1638
Size: 1300 338
EcoRV GAT'ATC
Cuts at: 0 231 1638
Size: 231 1407
HaeII r_GCGC'y
Cuts at: 0 59 1638
Size: 59 1579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1638
Size: 772 32 834
Fragments arranged by size:
834 772 32
HincII GTy'rAC
Cuts at: 0 1170 1638
Size: 1170 468
HindIII A'AGCT_T
Cuts at: 0 1073 1638
Size: 1073 565
KasI G'GCGC_C
Cuts at: 0 55 1638
Size: 55 1583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1638
Size: 671 967
MslI CAynn'nnrTG
Cuts at: 0 1273 1638
Size: 1273 365
MspA1I CmG'CkG
Cuts at: 0 72 991 1638
Size: 72 919 647
Fragments arranged by size:
919 647 72
NarI GG'CG_CC
Cuts at: 0 56 1638
Size: 56 1582
NcoI C'CATG_G
Cuts at: 0 113 1154 1638
Size: 113 1041 484
Fragments arranged by size:
1041 484 113
NspI r_CATG'y
Cuts at: 0 1086 1638
Size: 1086 552
PasI CC'CwG_GG
Cuts at: 0 549 1638
Size: 549 1089
PciI A'CATG_T
Cuts at: 0 1082 1638
Size: 1082 556
PflMI CCAn_nnn'nTGG
Cuts at: 0 1549 1638
Size: 1549 89
PpuMI rG'GwC_Cy
Cuts at: 0 545 571 1638
Size: 545 26 1067
Fragments arranged by size:
1067 545 26
PspOMI G'GGCC_C
Cuts at: 0 498 1638
Size: 498 1140
PspXI vC'TCGA_Gb
Cuts at: 0 746 1638
Size: 746 892
SacI G_AGCT'C
Cuts at: 0 1538 1638
Size: 1538 100
SanDI GG'GwC_CC
Cuts at: 0 545 1638
Size: 545 1093
SapI GCTCTTCn'nnn_
Cuts at: 0 455 729 1424 1638
Size: 455 274 695 214
Fragments arranged by size:
695 455 274 214
SfcI C'TryA_G
Cuts at: 0 268 849 1638
Size: 268 581 789
Fragments arranged by size:
789 581 268
SfoI GGC'GCC
Cuts at: 0 57 1638
Size: 57 1581
SmlI C'TyrA_G
Cuts at: 0 746 1638
Size: 746 892
StuI AGG'CCT
Cuts at: 0 250 628 1638
Size: 250 378 1010
Fragments arranged by size:
1010 378 250
StyI C'CwwG_G
Cuts at: 0 113 623 1154 1638
Size: 113 510 531 484
Fragments arranged by size:
531 510 484 113
TatI w'GTAC_w
Cuts at: 0 206 1638
Size: 206 1432
Tth111I GACn'n_nGTC
Cuts at: 0 49 1638
Size: 49 1589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 514 556 1546 1638
Size: 514 42 990 92
Fragments arranged by size:
990 514 92 42
XhoI C'TCGA_G
Cuts at: 0 746 1638
Size: 746 892
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BclI BglI Bme1580I BplI BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BstAPI BstEII Bsu36I BtgI
BtsI CspCI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII HindIII KasI MmeI MslI
MspA1I NarI NcoI NspI PasI PciI PflMI PpuMI
PspOMI PspXI SacI SanDI SapI SfcI SfoI SmlI
StuI StyI TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I BtgZI ClaI DraI
DraIII DrdI EaeI EagI EcoNI FalI FseI FspI
FspAI HpaI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI XbaI XmaI XmnI ZraI