Restriction map for
pFN21AE5616
(Linear) (Six-base) MAPSORT of: KIEE5616.seq Check: 4133 from: 1 to: 1506
KIEE5616.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1446 1506
Size: 1446 60
AccI GT'mk_AC
Cuts at: 0 1167 1506
Size: 1167 339
Acc65I G'GTAC_C
Cuts at: 0 293 1506
Size: 293 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1264 1299 1506
Size: 468 796 35 207
Fragments arranged by size:
796 468 207 35
AleI CACnn'nnGTG
Cuts at: 0 695 1506
Size: 695 811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1153 1506
Size: 1153 353
AlwNI CAG_nnn'CTG
Cuts at: 0 1210 1332 1386 1506
Size: 1210 122 54 120
Fragments arranged by size:
1210 122 120 54
ApaI G_GGCC'C
Cuts at: 0 498 1506
Size: 498 1008
BamHI G'GATC_C
Cuts at: 0 1465 1506
Size: 1465 41
BanI G'GyrC_C
Cuts at: 0 293 1506
Size: 293 1213
BanII G_rGCy'C
Cuts at: 0 498 968 1506
Size: 498 470 538
Fragments arranged by size:
538 498 470
BbsI GAAGACnn'nnnn_
Cuts at: 0 474 566 1506
Size: 474 92 940
Fragments arranged by size:
940 474 92
BbvCI CC'TCA_GC
Cuts at: 0 263 1506
Size: 263 1243
BciVI GTATCCnnnnn_n'
Cuts at: 0 1383 1506
Size: 1383 123
BfrBI ATG'CAT
Cuts at: 0 209 1506
Size: 209 1297
BlpI GC'TnA_GC
Cuts at: 0 23 1336 1506
Size: 23 1313 170
Fragments arranged by size:
1313 170 23
Bme1580I G_kGCm'C
Cuts at: 0 498 942 1003 1506
Size: 498 444 61 503
Fragments arranged by size:
503 498 444 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 262 1506
Size: 262 1244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 295 393 797 815 824 1218 1458
Size: 110 185 98 404 18 9 394 240
Cuts at: 1458 1506
Size: 48
Fragments arranged by size:
404 394 240 185 110 98 48 18
9
Bpu10I CC'TnA_GC
Cuts at: 0 74 263 1211 1506
Size: 74 189 948 295
Fragments arranged by size:
948 295 189 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 616 1506
Size: 472 144 890
Fragments arranged by size:
890 472 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 380 1506
Size: 380 1126
BsaWI w'CCGG_w
Cuts at: 0 811 1448 1485 1506
Size: 811 637 37 21
Fragments arranged by size:
811 637 37 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1402 1432 1506
Size: 1402 30 74
Fragments arranged by size:
1402 74 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1223 1399 1506
Size: 1159 64 176 107
Fragments arranged by size:
1159 176 107 64
BsiHKAI G_wGCw'C
Cuts at: 0 1032 1444 1506
Size: 1032 412 62
Fragments arranged by size:
1032 412 62
Bsp1286I G_dGCh'C
Cuts at: 0 498 942 968 1003 1032 1444 1506
Size: 498 444 26 35 29 412 62
Fragments arranged by size:
498 444 412 62 35 29 26
BspEI T'CCGG_A
Cuts at: 0 811 1448 1506
Size: 811 637 58
Fragments arranged by size:
811 637 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1446 1506
Size: 1446 60
BsrFI r'CCGG_y
Cuts at: 0 159 1506
Size: 159 1347
BstEII G'GTnAC_C
Cuts at: 0 621 1506
Size: 621 885
BstYI r'GATC_y
Cuts at: 0 1288 1465 1506
Size: 1288 177 41
Fragments arranged by size:
1288 177 41
Bsu36I CC'TnA_GG
Cuts at: 0 1372 1506
Size: 1372 134
BtgI C'CryG_G
Cuts at: 0 8 867 1506
Size: 8 859 639
Fragments arranged by size:
859 639 8
BtsI GCAGTG_nn'
Cuts at: 0 1137 1506
Size: 1137 369
DraIII CAC_nnn'GTG
Cuts at: 0 424 840 1506
Size: 424 416 666
Fragments arranged by size:
666 424 416
EaeI y'GGCC_r
Cuts at: 0 1360 1506
Size: 1360 146
EarI CTCTTCn'nnn_
Cuts at: 0 956 1318 1506
Size: 956 362 188
Fragments arranged by size:
956 362 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 295 393 468 797 815 824 1218
Size: 110 185 98 75 329 18 9 394
Cuts at: 1218 1264 1299 1458 1506
Size: 46 35 159 48
Fragments arranged by size:
394 329 185 159 110 98 75 48
46 35 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1506
Size: 331 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 374 406 905 937 1280 1312
Size: 108 32 234 32 499 32 343 32
Cuts at: 1312 1402 1434 1457 1489 1506
Size: 90 32 23 32 17
Fragments arranged by size:
499 343 234 108 90 32 32 32
32 32 32 23 17
HincII GTy'rAC
Cuts at: 0 1168 1506
Size: 1168 338
KpnI G_GTAC'C
Cuts at: 0 297 1506
Size: 297 1209
MfeI C'AATT_G
Cuts at: 0 1268 1506
Size: 1268 238
MscI TGG'CCA
Cuts at: 0 1362 1506
Size: 1362 144
MslI CAynn'nnrTG
Cuts at: 0 397 445 695 1506
Size: 397 48 250 811
Fragments arranged by size:
811 397 250 48
MspA1I CmG'CkG
Cuts at: 0 10 106 130 695 982 1196 1506
Size: 10 96 24 565 287 214 310
Fragments arranged by size:
565 310 287 214 96 24 10
NsiI A_TGCA'T
Cuts at: 0 211 1506
Size: 211 1295
PflMI CCAn_nnn'nTGG
Cuts at: 0 868 1196 1506
Size: 868 328 310
Fragments arranged by size:
868 328 310
PfoI T'CCnGG_A
Cuts at: 0 537 1468 1506
Size: 537 931 38
Fragments arranged by size:
931 537 38
PspOMI G'GGCC_C
Cuts at: 0 494 1506
Size: 494 1012
PstI C_TGCA'G
Cuts at: 0 612 1103 1506
Size: 612 491 403
Fragments arranged by size:
612 491 403
PvuII CAG'CTG
Cuts at: 0 130 695 982 1196 1506
Size: 130 565 287 214 310
Fragments arranged by size:
565 310 287 214 130
SacII CC_GC'GG
Cuts at: 0 11 1506
Size: 11 1495
SalI G'TCGA_C
Cuts at: 0 1166 1506
Size: 1166 340
SapI GCTCTTCn'nnn_
Cuts at: 0 956 1506
Size: 956 550
SexAI A'CCwGG_T
Cuts at: 0 836 1506
Size: 836 670
SfcI C'TryA_G
Cuts at: 0 608 707 894 1099 1506
Size: 608 99 187 205 407
Fragments arranged by size:
608 407 205 187 99
SmlI C'TyrA_G
Cuts at: 0 451 595 1506
Size: 451 144 911
Fragments arranged by size:
911 451 144
StuI AGG'CCT
Cuts at: 0 139 1506
Size: 139 1367
StyI C'CwwG_G
Cuts at: 0 933 1506
Size: 933 573
TatI w'GTAC_w
Cuts at: 0 1403 1506
Size: 1403 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 1506
Size: 671 32 803
Fragments arranged by size:
803 671 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 86 421 525 649 1506
Size: 86 335 104 124 857
Fragments arranged by size:
857 335 124 104 86
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AlwNI ApaI
BamHI BanI BanII BbsI BbvCI BciVI BfrBI BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstEII BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI
Hin4I HincII KpnI MfeI MscI MslI MspA1I NsiI
PflMI PfoI PspOMI PstI PvuII SacII SalI SapI
SexAI SfcI SmlI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BclI BglI BglII BmgBI BmrI BmtI
BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI