Restriction map for
pFN21AE5611
(Linear) (Six-base) MAPSORT of: KIEE5611.seq Check: 3942 from: 1 to: 1476
KIEE5611.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 1476
Size: 742 734
AatII G_ACGT'C
Cuts at: 0 614 1476
Size: 614 862
AccI GT'mk_AC
Cuts at: 0 938 1476
Size: 938 538
AfeI AGC'GCT
Cuts at: 0 100 139 166 1476
Size: 100 39 27 1310
Fragments arranged by size:
1310 100 39 27
AflIII A'CryG_T
Cuts at: 0 239 1476
Size: 239 1237
AleI CACnn'nnGTG
Cuts at: 0 992 1476
Size: 992 484
AlwNI CAG_nnn'CTG
Cuts at: 0 117 188 402 744 1037 1196 1476
Size: 117 71 214 342 293 159 280
Fragments arranged by size:
342 293 280 214 159 117 71
ApaI G_GGCC'C
Cuts at: 0 21 69 78 209 453 1463 1476
Size: 21 48 9 131 244 1010 13
Fragments arranged by size:
1010 244 131 48 21 13 9
AvaI C'yCGr_G
Cuts at: 0 40 261 445 948 1476
Size: 40 221 184 503 528
Fragments arranged by size:
528 503 221 184 40
BanI G'GyrC_C
Cuts at: 0 730 1015 1476
Size: 730 285 461
Fragments arranged by size:
730 461 285
BanII G_rGCy'C
Cuts at: 0 7 21 69 78 209 300 453 955
Size: 7 14 48 9 131 91 153 502
Cuts at: 955 1130 1463 1476
Size: 175 333 13
Fragments arranged by size:
502 333 175 153 131 91 48 14
13 9 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 1476
Size: 300 1176
BbvCI CC'TCA_GC
Cuts at: 0 161 1476
Size: 161 1315
BciVI GTATCCnnnnn_n'
Cuts at: 0 533 1476
Size: 533 943
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1179 1476
Size: 73 1106 297
Fragments arranged by size:
1106 297 73
Bme1580I G_kGCm'C
Cuts at: 0 21 69 78 209 374 453 1463 1476
Size: 21 48 9 131 165 79 1010 13
Fragments arranged by size:
1010 165 131 79 48 21 13 9
BmgBI CAC'GTC
Cuts at: 0 240 1476
Size: 240 1236
BmrI ACTGGGnnnn_n'
Cuts at: 0 233 1476
Size: 233 1243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1411 1476
Size: 40 1371 65
Fragments arranged by size:
1371 65 40
Bpu10I CC'TnA_GC
Cuts at: 0 161 1476
Size: 161 1315
BsaHI Gr'CG_yC
Cuts at: 0 611 1028 1476
Size: 611 417 448
Fragments arranged by size:
611 448 417
BseYI C'CCAG_C
Cuts at: 0 719 767 1476
Size: 719 48 709
Fragments arranged by size:
719 709 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1476
Size: 1038 438
BsiEI CG_ry'CG
Cuts at: 0 287 1476
Size: 287 1189
BsiHKAI G_wGCw'C
Cuts at: 0 320 924 962 1130 1476
Size: 320 604 38 168 346
Fragments arranged by size:
604 346 320 168 38
BsmI GAATG_Cn'
Cuts at: 0 795 1476
Size: 795 681
Bsp1286I G_dGCh'C
Cuts at: 0 7 21 69 78 209 300 320 374
Size: 7 14 48 9 131 91 20 54
Cuts at: 374 453 924 955 962 1130 1463 1476
Size: 79 471 31 7 168 333 13
Fragments arranged by size:
471 333 168 131 91 79 54 48
31 20 14 13 9 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 1118 1476
Size: 742 376 358
Fragments arranged by size:
742 376 358
BsrBI CCG'CTC
Cuts at: 0 10 291 1476
Size: 10 281 1185
Fragments arranged by size:
1185 281 10
BsrFI r'CCGG_y
Cuts at: 0 977 1018 1476
Size: 977 41 458
Fragments arranged by size:
977 458 41
BssHII G'CGCG_C
Cuts at: 0 428 557 1476
Size: 428 129 919
Fragments arranged by size:
919 428 129
BstYI r'GATC_y
Cuts at: 0 1359 1476
Size: 1359 117
Bsu36I CC'TnA_GG
Cuts at: 0 511 1476
Size: 511 965
BtgI C'CryG_G
Cuts at: 0 1370 1476
Size: 1370 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 453 1284 1476
Size: 453 831 192
Fragments arranged by size:
831 453 192
BtsI GCAGTG_nn'
Cuts at: 0 960 1476
Size: 960 516
DraIII CAC_nnn'GTG
Cuts at: 0 534 1476
Size: 534 942
EaeI y'GGCC_r
Cuts at: 0 284 404 551 806 1476
Size: 284 120 147 255 670
Fragments arranged by size:
670 284 255 147 120
EagI C'GGCC_G
Cuts at: 0 284 1476
Size: 284 1192
EarI CTCTTCn'nnn_
Cuts at: 0 778 1476
Size: 778 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 634 1120 1476
Size: 634 486 356
Fragments arranged by size:
634 486 356
EcoICRI GAG'CTC
Cuts at: 0 1128 1476
Size: 1128 348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1411 1476
Size: 40 1371 65
Fragments arranged by size:
1371 65 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 510 1476
Size: 510 966
EcoO109I rG'GnC_Cy
Cuts at: 0 18 65 74 75 205 399 449 759
Size: 18 47 9 1 130 194 50 310
Cuts at: 759 844 1034 1459 1476
Size: 85 190 425 17
Fragments arranged by size:
425 310 194 190 130 85 50 47
18 17 9 1
FspI TGC'GCA
Cuts at: 0 353 1476
Size: 353 1123
HaeII r_GCGC'y
Cuts at: 0 40 102 141 168 547 1476
Size: 40 62 39 27 379 929
Fragments arranged by size:
929 379 62 40 39 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 428 460 507 539 1457 1476
Size: 144 32 252 32 47 32 918 19
Fragments arranged by size:
918 252 144 47 32 32 32 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 617 696 1262 1476
Size: 158 459 79 566 214
Fragments arranged by size:
566 459 214 158 79
MscI TGG'CCA
Cuts at: 0 808 1476
Size: 808 668
MslI CAynn'nnrTG
Cuts at: 0 992 1476
Size: 992 484
MspA1I CmG'CkG
Cuts at: 0 719 744 992 1137 1476
Size: 719 25 248 145 339
Fragments arranged by size:
719 339 248 145 25
PasI CC'CwG_GG
Cuts at: 0 14 651 1256 1476
Size: 14 637 605 220
Fragments arranged by size:
637 605 220 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 1037 1196 1476
Size: 534 503 159 280
Fragments arranged by size:
534 503 280 159
PfoI T'CCnGG_A
Cuts at: 0 92 605 1476
Size: 92 513 871
Fragments arranged by size:
871 513 92
PpuMI rG'GwC_Cy
Cuts at: 0 759 844 1476
Size: 759 85 632
Fragments arranged by size:
759 632 85
PspOMI G'GGCC_C
Cuts at: 0 17 65 74 205 449 1459 1476
Size: 17 48 9 131 244 1010 17
Fragments arranged by size:
1010 244 131 48 17 17 9
PvuII CAG'CTG
Cuts at: 0 992 1476
Size: 992 484
RsrII CG'GwC_CG
Cuts at: 0 714 1476
Size: 714 762
SacI G_AGCT'C
Cuts at: 0 1130 1476
Size: 1130 346
SanDI GG'GwC_CC
Cuts at: 0 844 1476
Size: 844 632
SapI GCTCTTCn'nnn_
Cuts at: 0 778 1476
Size: 778 698
SexAI A'CCwGG_T
Cuts at: 0 530 1476
Size: 530 946
SfcI C'TryA_G
Cuts at: 0 694 1476
Size: 694 782
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 73 1476
Size: 73 1403
StuI AGG'CCT
Cuts at: 0 581 1051 1476
Size: 581 470 425
Fragments arranged by size:
581 470 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 1108 1367 1476
Size: 664 444 259 109
Fragments arranged by size:
664 444 259 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 486 518 1272 1304 1476
Size: 486 32 754 32 172
Fragments arranged by size:
754 486 172 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 712 1476
Size: 712 764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 837 1245 1476
Size: 837 408 231
Fragments arranged by size:
837 408 231
XmnI GAAnn'nnTTC
Cuts at: 0 793 1476
Size: 793 683
ZraI GAC'GTC
Cuts at: 0 612 1476
Size: 612 864
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AleI AlwNI ApaI
AvaI BanI BanII BbsI BbvCI BciVI BglI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaHI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MmeI MscI MslI MspA1I PasI PflMI PfoI PpuMI
PspOMI PvuII RsrII SacI SanDI SapI SexAI SfcI
SfiI StuI TaqII TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BclI BfrBI BglII BlpI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
SacII SalI SbfI ScaI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI XbaI XhoI XmaI