Restriction map for
pFN21AE5610
(Linear) (Six-base) MAPSORT of: KIEE5610.seq Check: 3836 from: 1 to: 1752
KIEE5610.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1555 1615 1752
Size: 502 1053 60 137
Fragments arranged by size:
1053 502 137 60
AflIII A'CryG_T
Cuts at: 0 887 1752
Size: 887 865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1192 1752
Size: 1192 560
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 742 774 1752
Size: 742 32 978
Fragments arranged by size:
978 742 32
AlwNI CAG_nnn'CTG
Cuts at: 0 184 1561 1752
Size: 184 1377 191
Fragments arranged by size:
1377 191 184
AvaI C'yCGr_G
Cuts at: 0 307 1047 1072 1752
Size: 307 740 25 680
Fragments arranged by size:
740 680 307 25
BamHI G'GATC_C
Cuts at: 0 1305 1752
Size: 1305 447
BanII G_rGCy'C
Cuts at: 0 143 426 1079 1442 1752
Size: 143 283 653 363 310
Fragments arranged by size:
653 363 310 283 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 707 734 1369 1752
Size: 707 27 635 383
Fragments arranged by size:
707 635 383 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 413 1752
Size: 379 34 1339
Fragments arranged by size:
1339 379 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 248 1752
Size: 248 1504
BclI T'GATC_A
Cuts at: 0 105 1752
Size: 105 1647
BglI GCCn_nnn'nGGC
Cuts at: 0 1615 1752
Size: 1615 137
BglII A'GATC_T
Cuts at: 0 588 1752
Size: 588 1164
BlpI GC'TnA_GC
Cuts at: 0 1003 1752
Size: 1003 749
BmgBI CAC'GTC
Cuts at: 0 473 1752
Size: 473 1279
BmtI G_CTAG'C
Cuts at: 0 1592 1752
Size: 1592 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 679 1118 1237 1752
Size: 244 435 439 119 515
Fragments arranged by size:
515 439 435 244 119
BsaAI yAC'GTr
Cuts at: 0 192 697 1752
Size: 192 505 1055
Fragments arranged by size:
1055 505 192
BsaWI w'CCGG_w
Cuts at: 0 1520 1752
Size: 1520 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 877 907 1449 1479 1752
Size: 742 30 105 30 542 30 273
Fragments arranged by size:
742 542 273 105 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 685 1752
Size: 151 534 1067
Fragments arranged by size:
1067 534 151
BseYI C'CCAG_C
Cuts at: 0 1161 1752
Size: 1161 591
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1657 1752
Size: 1657 95
BsiEI CG_ry'CG
Cuts at: 0 1241 1752
Size: 1241 511
BsiHKAI G_wGCw'C
Cuts at: 0 143 308 506 1752
Size: 143 165 198 1246
Fragments arranged by size:
1246 198 165 143
Bsp1286I G_dGCh'C
Cuts at: 0 143 308 426 506 1079 1442 1752
Size: 143 165 118 80 573 363 310
Fragments arranged by size:
573 363 310 165 143 118 80
BspEI T'CCGG_A
Cuts at: 0 1520 1752
Size: 1520 232
BspHI T'CATG_A
Cuts at: 0 1355 1752
Size: 1355 397
BsrDI GCAATG_nn'
Cuts at: 0 560 814 1752
Size: 560 254 938
Fragments arranged by size:
938 560 254
BsrGI T'GTAC_A
Cuts at: 0 248 1752
Size: 248 1504
BstAPI GCAn_nnn'nTGC
Cuts at: 0 969 1752
Size: 969 783
BstEII G'GTnAC_C
Cuts at: 0 202 1509 1752
Size: 202 1307 243
Fragments arranged by size:
1307 243 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 1192 1392 1752
Size: 408 784 200 360
Fragments arranged by size:
784 408 360 200
BstYI r'GATC_y
Cuts at: 0 588 1305 1640 1752
Size: 588 717 335 112
Fragments arranged by size:
717 588 335 112
Bsu36I CC'TnA_GG
Cuts at: 0 374 1752
Size: 374 1378
BtgI C'CryG_G
Cuts at: 0 1366 1514 1752
Size: 1366 148 238
Fragments arranged by size:
1366 238 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1593 1752
Size: 1593 159
BtsI GCAGTG_nn'
Cuts at: 0 185 1230 1752
Size: 185 1045 522
Fragments arranged by size:
1045 522 185
DraIII CAC_nnn'GTG
Cuts at: 0 257 1752
Size: 257 1495
EaeI y'GGCC_r
Cuts at: 0 35 1194 1752
Size: 35 1159 558
Fragments arranged by size:
1159 558 35
EarI CTCTTCn'nnn_
Cuts at: 0 593 662 1186 1679 1752
Size: 593 69 524 493 73
Fragments arranged by size:
593 524 493 73 69
EcoICRI GAG'CTC
Cuts at: 0 141 1752
Size: 141 1611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 502 679 1118 1237 1555 1615 1752
Size: 244 258 177 439 119 318 60 137
Fragments arranged by size:
439 318 258 244 177 137 119 60
EcoO109I rG'GnC_Cy
Cuts at: 0 744 1728 1752
Size: 744 984 24
Fragments arranged by size:
984 744 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1227 1441 1752
Size: 1227 214 311
Fragments arranged by size:
1227 311 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 592 624 1290 1322 1449 1481
Size: 97 32 463 32 666 32 127 32
Cuts at: 1481 1537 1569 1752
Size: 56 32 183
Fragments arranged by size:
666 463 183 127 97 56 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 61 1360 1752
Size: 61 1299 392
Fragments arranged by size:
1299 392 61
MfeI C'AATT_G
Cuts at: 0 1469 1752
Size: 1469 283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 53 265 1752
Size: 29 24 212 1487
Fragments arranged by size:
1487 212 29 24
MscI TGG'CCA
Cuts at: 0 1196 1752
Size: 1196 556
MspA1I CmG'CkG
Cuts at: 0 460 1752
Size: 460 1292
NcoI C'CATG_G
Cuts at: 0 1366 1752
Size: 1366 386
NheI G'CTAG_C
Cuts at: 0 1588 1752
Size: 1588 164
NspI r_CATG'y
Cuts at: 0 823 891 1752
Size: 823 68 861
Fragments arranged by size:
861 823 68
PasI CC'CwG_GG
Cuts at: 0 1612 1752
Size: 1612 140
PciI A'CATG_T
Cuts at: 0 887 1752
Size: 887 865
PfoI T'CCnGG_A
Cuts at: 0 1100 1184 1428 1752
Size: 1100 84 244 324
Fragments arranged by size:
1100 324 244 84
PmlI CAC'GTG
Cuts at: 0 697 1752
Size: 697 1055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1752
Size: 742 32 978
Fragments arranged by size:
978 742 32
PshAI GACnn'nnGTC
Cuts at: 0 1516 1752
Size: 1516 236
PstI C_TGCA'G
Cuts at: 0 635 965 1752
Size: 635 330 787
Fragments arranged by size:
787 635 330
PvuI CG_AT'CG
Cuts at: 0 1241 1752
Size: 1241 511
PvuII CAG'CTG
Cuts at: 0 460 1752
Size: 460 1292
SacI G_AGCT'C
Cuts at: 0 143 1752
Size: 143 1609
SapI GCTCTTCn'nnn_
Cuts at: 0 1186 1752
Size: 1186 566
SbfI CC_TGCA'GG
Cuts at: 0 635 1752
Size: 635 1117
SexAI A'CCwGG_T
Cuts at: 0 1268 1384 1652 1752
Size: 1268 116 268 100
Fragments arranged by size:
1268 268 116 100
SfcI C'TryA_G
Cuts at: 0 428 631 961 1752
Size: 428 203 330 791
Fragments arranged by size:
791 428 330 203
SmlI C'TyrA_G
Cuts at: 0 307 1752
Size: 307 1445
StuI AGG'CCT
Cuts at: 0 13 479 1534 1752
Size: 13 466 1055 218
Fragments arranged by size:
1055 466 218 13
StyI C'CwwG_G
Cuts at: 0 8 96 876 1366 1619 1713 1752
Size: 8 88 780 490 253 94 39
Fragments arranged by size:
780 490 253 94 88 39 8
TatI w'GTAC_w
Cuts at: 0 248 1752
Size: 248 1504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 877 909 1587 1619 1752
Size: 511 32 334 32 678 32 133
Fragments arranged by size:
678 511 334 133 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1719 1752
Size: 1719 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 567 1752
Size: 408 159 1185
Fragments arranged by size:
1185 408 159
XhoI C'TCGA_G
Cuts at: 0 307 1752
Size: 307 1445
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI AvaI BamHI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI BpmI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrDI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII
MfeI MmeI MscI MspA1I NcoI NheI NspI PasI
PciI PfoI PmlI PpiI PshAI PstI PvuI PvuII
SacI SapI SbfI SexAI SfcI SmlI StuI StyI
TatI TstI XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BfrBI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsiWI BsmI
BsmBI BspMI BsrBI BsrFI BssHII BssSI BstBI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MluI MslI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PflMI PmeI PpuMI PsiI PspOMI
PspXI PsrI RsrII SacII SalI SanDI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XmaI XmnI ZraI