Restriction map for
pFN21AE5609
(Linear) (Six-base) MAPSORT of: KIEE5609.seq Check: 4382 from: 1 to: 1599
KIEE5609.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 1599
Size: 114 1485
AccI GT'mk_AC
Cuts at: 0 788 1599
Size: 788 811
ApoI r'AATT_y
Cuts at: 0 79 557 1087 1520 1599
Size: 79 478 530 433 79
Fragments arranged by size:
530 478 433 79 79
AseI AT'TA_AT
Cuts at: 0 1333 1599
Size: 1333 266
BamHI G'GATC_C
Cuts at: 0 1591 1599
Size: 1591 8
BanI G'GyrC_C
Cuts at: 0 102 1599
Size: 102 1497
BanII G_rGCy'C
Cuts at: 0 50 1599
Size: 50 1549
BciVI GTATCCnnnnn_n'
Cuts at: 0 1175 1599
Size: 1175 424
BclI T'GATC_A
Cuts at: 0 861 1599
Size: 861 738
BglII A'GATC_T
Cuts at: 0 930 1599
Size: 930 669
Bpu10I CC'TnA_GC
Cuts at: 0 496 821 1599
Size: 496 325 778
Fragments arranged by size:
778 496 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1599
Size: 911 688
BsaAI yAC'GTr
Cuts at: 0 1231 1599
Size: 1231 368
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 1599
Size: 112 1487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1399 1599
Size: 1399 200
BsiHKAI G_wGCw'C
Cuts at: 0 50 1599
Size: 50 1549
BsmI GAATG_Cn'
Cuts at: 0 201 1599
Size: 201 1398
Bsp1286I G_dGCh'C
Cuts at: 0 50 1599
Size: 50 1549
BspHI T'CATG_A
Cuts at: 0 851 1599
Size: 851 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 1599
Size: 114 1485
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1135 1599
Size: 1135 464
BstBI TT'CG_AA
Cuts at: 0 1393 1599
Size: 1393 206
BstYI r'GATC_y
Cuts at: 0 930 1591 1599
Size: 930 661 8
Fragments arranged by size:
930 661 8
BtgI C'CryG_G
Cuts at: 0 1541 1599
Size: 1541 58
BtsI GCAGTG_nn'
Cuts at: 0 807 1599
Size: 807 792
DraI TTT'AAA
Cuts at: 0 84 1599
Size: 84 1515
EarI CTCTTCn'nnn_
Cuts at: 0 1111 1599
Size: 1111 488
EcoICRI GAG'CTC
Cuts at: 0 48 1599
Size: 48 1551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 734 1287 1599
Size: 734 553 312
Fragments arranged by size:
734 553 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 1169 1201 1405 1437 1509 1541
Size: 846 32 291 32 204 32 72 32
Cuts at: 1541 1599
Size: 58
Fragments arranged by size:
846 291 204 72 58 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 206 1416 1599
Size: 206 1210 183
Fragments arranged by size:
1210 206 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1599
Size: 43 1556
MslI CAynn'nnrTG
Cuts at: 0 442 1599
Size: 442 1157
NdeI CA'TA_TG
Cuts at: 0 174 1599
Size: 174 1425
PflMI CCAn_nnn'nTGG
Cuts at: 0 706 1599
Size: 706 893
PsiI TTA'TAA
Cuts at: 0 1192 1599
Size: 1192 407
SacI G_AGCT'C
Cuts at: 0 50 1599
Size: 50 1549
SfcI C'TryA_G
Cuts at: 0 723 1599
Size: 723 876
SmlI C'TyrA_G
Cuts at: 0 926 1599
Size: 926 673
SspI AAT'ATT
Cuts at: 0 504 1331 1599
Size: 504 827 268
Fragments arranged by size:
827 504 268
StyI C'CwwG_G
Cuts at: 0 902 1599
Size: 902 697
XbaI T'CTAG_A
Cuts at: 0 756 1599
Size: 756 843
XmnI GAAnn'nnTTC
Cuts at: 0 1088 1296 1599
Size: 1088 208 303
Fragments arranged by size:
1088 303 208
Enzymes that do cut:
AarI AccI ApoI AseI BamHI BanI BanII BciVI
BclI BglII Bpu10I BpuEI BsaAI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstAPI BstBI BstYI BtgI
BtsI DraI EarI EcoICRI FalI Hin4I MfeI MmeI
MslI NdeI PflMI PsiI SacI SfcI SmlI SspI
StyI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MscI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI