Restriction map for
pFN21AE5607
(Linear) (Six-base) MAPSORT of: KIEE5607.seq Check: 1398 from: 1 to: 1758
KIEE5607.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1037 1202 1706 1758
Size: 188 849 165 504 52
Fragments arranged by size:
849 504 188 165 52
AflIII A'CryG_T
Cuts at: 0 459 1758
Size: 459 1299
ApoI r'AATT_y
Cuts at: 0 324 427 675 756 828 840 1758
Size: 324 103 248 81 72 12 918
Fragments arranged by size:
918 324 248 103 81 72 12
AvaI C'yCGr_G
Cuts at: 0 1673 1758
Size: 1673 85
AvrII C'CTAG_G
Cuts at: 0 409 1758
Size: 409 1349
BbsI GAAGACnn'nnnn_
Cuts at: 0 1190 1758
Size: 1190 568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 23 57 1758
Size: 23 34 1701
Fragments arranged by size:
1701 34 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 1318 1758
Size: 1318 440
BmrI ACTGGGnnnn_n'
Cuts at: 0 1433 1758
Size: 1433 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 314 434 950 1139 1388 1758
Size: 117 197 120 516 189 249 370
Fragments arranged by size:
516 370 249 197 189 120 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1185 1758
Size: 1185 573
BsaI GGTCTCn'nnnn_
Cuts at: 0 249 399 1615 1758
Size: 249 150 1216 143
Fragments arranged by size:
1216 249 150 143
BsaWI w'CCGG_w
Cuts at: 0 785 1758
Size: 785 973
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1273 1414 1609 1758
Size: 1273 141 195 149
Fragments arranged by size:
1273 195 149 141
BseYI C'CCAG_C
Cuts at: 0 1375 1758
Size: 1375 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 1758
Size: 17 1741
BsmBI CGTCTCn'nnnn_
Cuts at: 0 765 1758
Size: 765 993
BspEI T'CCGG_A
Cuts at: 0 785 1758
Size: 785 973
BsrBI CCG'CTC
Cuts at: 0 1233 1758
Size: 1233 525
BsrDI GCAATG_nn'
Cuts at: 0 144 878 1758
Size: 144 734 880
Fragments arranged by size:
880 734 144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 28 1758
Size: 28 1730
BstXI CCAn_nnnn'nTGG
Cuts at: 0 969 1758
Size: 969 789
BstYI r'GATC_y
Cuts at: 0 834 1012 1035 1271 1758
Size: 834 178 23 236 487
Fragments arranged by size:
834 487 236 178 23
Bsu36I CC'TnA_GG
Cuts at: 0 615 1758
Size: 615 1143
BtgI C'CryG_G
Cuts at: 0 211 1736 1758
Size: 211 1525 22
Fragments arranged by size:
1525 211 22
DraI TTT'AAA
Cuts at: 0 245 1758
Size: 245 1513
EaeI y'GGCC_r
Cuts at: 0 1131 1758
Size: 1131 627
EarI CTCTTCn'nnn_
Cuts at: 0 490 542 659 1682 1758
Size: 490 52 117 1023 76
Fragments arranged by size:
1023 490 117 76 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 188 314 434 950 1037 1139 1202
Size: 117 71 126 120 516 87 102 63
Cuts at: 1202 1388 1706 1758
Size: 186 318 52
Fragments arranged by size:
516 318 186 126 120 117 102 87
71 63 52
EcoRI G'AATT_C
Cuts at: 0 324 1758
Size: 324 1434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 257 1758
Size: 257 1501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 867 899 997 1029 1263 1295 1758
Size: 867 32 98 32 234 32 463
Fragments arranged by size:
867 463 234 98 32 32 32
HincII GTy'rAC
Cuts at: 0 1392 1758
Size: 1392 366
HpaI GTT'AAC
Cuts at: 0 1392 1758
Size: 1392 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1758
Size: 399 1359
MscI TGG'CCA
Cuts at: 0 1133 1758
Size: 1133 625
MslI CAynn'nnrTG
Cuts at: 0 701 967 1145 1758
Size: 701 266 178 613
Fragments arranged by size:
701 613 266 178
MspA1I CmG'CkG
Cuts at: 0 1159 1758
Size: 1159 599
NcoI C'CATG_G
Cuts at: 0 211 1736 1758
Size: 211 1525 22
Fragments arranged by size:
1525 211 22
NspI r_CATG'y
Cuts at: 0 359 463 747 1665 1758
Size: 359 104 284 918 93
Fragments arranged by size:
918 359 284 104 93
PciI A'CATG_T
Cuts at: 0 459 1758
Size: 459 1299
PfoI T'CCnGG_A
Cuts at: 0 1115 1758
Size: 1115 643
PsiI TTA'TAA
Cuts at: 0 1411 1758
Size: 1411 347
PstI C_TGCA'G
Cuts at: 0 150 1758
Size: 150 1608
PvuII CAG'CTG
Cuts at: 0 1159 1758
Size: 1159 599
ScaI AGT'ACT
Cuts at: 0 1198 1758
Size: 1198 560
SfcI C'TryA_G
Cuts at: 0 146 1027 1758
Size: 146 881 731
Fragments arranged by size:
881 731 146
SmlI C'TyrA_G
Cuts at: 0 1200 1673 1758
Size: 1200 473 85
Fragments arranged by size:
1200 473 85
SspI AAT'ATT
Cuts at: 0 820 1758
Size: 820 938
StyI C'CwwG_G
Cuts at: 0 211 409 1736 1749 1758
Size: 211 198 1327 13 9
Fragments arranged by size:
1327 211 198 13 9
TatI w'GTAC_w
Cuts at: 0 1196 1363 1758
Size: 1196 167 395
Fragments arranged by size:
1196 395 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 1004 1036 1758
Size: 611 32 361 32 722
Fragments arranged by size:
722 611 361 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 653 1758
Size: 564 89 1105
Fragments arranged by size:
1105 564 89
XhoI C'TCGA_G
Cuts at: 0 1673 1758
Size: 1673 85
Enzymes that do cut:
AcuI AflIII ApoI AvaI AvrII BbsI BcgI BciVI
BmrI BpmI BpuEI BsaI BsaWI BseRI BseYI BsgI
BsmBI BspEI BsrBI BsrDI BstAPI BstXI BstYI Bsu36I
BtgI DraI EaeI EarI Eco57MI EcoRI FalI Hin4I
HincII HpaI MmeI MscI MslI MspA1I NcoI NspI
PciI PfoI PsiI PstI PvuII ScaI SfcI SmlI
SspI StyI TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI BaeI BamHI BanI BanII BbeI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XbaI XmaI XmnI ZraI