Restriction map for
pFN21AE5603
(Linear) (Six-base) MAPSORT of: KIEE5603.seq Check: 4719 from: 1 to: 1416
KIEE5603.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AccI GT'mk_AC
Cuts at: 0 295 1416
Size: 295 1121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 710 1416
Size: 422 288 706
Fragments arranged by size:
706 422 288
AflIII A'CryG_T
Cuts at: 0 777 1416
Size: 777 639
ApaLI G'TGCA_C
Cuts at: 0 859 1416
Size: 859 557
ApoI r'AATT_y
Cuts at: 0 424 490 1416
Size: 424 66 926
Fragments arranged by size:
926 424 66
AvaI C'yCGr_G
Cuts at: 0 1228 1416
Size: 1228 188
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 233 266 1416
Size: 233 33 1150
Fragments arranged by size:
1150 233 33
BanII G_rGCy'C
Cuts at: 0 976 1416
Size: 976 440
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 698 1416
Size: 153 545 718
Fragments arranged by size:
718 545 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 996 1030 1416
Size: 996 34 386
Fragments arranged by size:
996 386 34
BclI T'GATC_A
Cuts at: 0 867 1416
Size: 867 549
Bme1580I G_kGCm'C
Cuts at: 0 863 1416
Size: 863 553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 1099 1254 1416
Size: 63 1036 155 162
Fragments arranged by size:
1036 162 155 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 233 494 1416
Size: 58 175 261 922
Fragments arranged by size:
922 261 175 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 1148 1416
Size: 1148 268
BsaAI yAC'GTr
Cuts at: 0 299 1255 1416
Size: 299 956 161
Fragments arranged by size:
956 299 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 206 236 1416
Size: 206 30 1180
Fragments arranged by size:
1180 206 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 399 1416
Size: 399 1017
BseYI C'CCAG_C
Cuts at: 0 141 1416
Size: 141 1275
BsiHKAI G_wGCw'C
Cuts at: 0 863 1416
Size: 863 553
BsmI GAATG_Cn'
Cuts at: 0 999 1050 1416
Size: 999 51 366
Fragments arranged by size:
999 366 51
Bsp1286I G_dGCh'C
Cuts at: 0 863 976 1416
Size: 863 113 440
Fragments arranged by size:
863 440 113
BsrGI T'GTAC_A
Cuts at: 0 1358 1416
Size: 1358 58
BssSI C'ACGA_G
Cuts at: 0 314 1416
Size: 314 1102
BstEII G'GTnAC_C
Cuts at: 0 375 1416
Size: 375 1041
BstXI CCAn_nnnn'nTGG
Cuts at: 0 349 1416
Size: 349 1067
BstYI r'GATC_y
Cuts at: 0 207 817 1133 1416
Size: 207 610 316 283
Fragments arranged by size:
610 316 283 207
BtgI C'CryG_G
Cuts at: 0 924 1416
Size: 924 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1142 1416
Size: 1142 274
BtsI GCAGTG_nn'
Cuts at: 0 28 254 1416
Size: 28 226 1162
Fragments arranged by size:
1162 226 28
DraI TTT'AAA
Cuts at: 0 719 1416
Size: 719 697
DraIII CAC_nnn'GTG
Cuts at: 0 136 1416
Size: 136 1280
EaeI y'GGCC_r
Cuts at: 0 114 1416
Size: 114 1302
EarI CTCTTCn'nnn_
Cuts at: 0 822 956 1416
Size: 822 134 460
Fragments arranged by size:
822 460 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 233 422 494 710 1416
Size: 58 175 189 72 216 706
Fragments arranged by size:
706 216 189 175 72 58
EcoO109I rG'GnC_Cy
Cuts at: 0 727 1010 1416
Size: 727 283 406
Fragments arranged by size:
727 406 283
HaeII r_GCGC'y
Cuts at: 0 19 37 1416
Size: 19 18 1379
Fragments arranged by size:
1379 19 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 1416
Size: 835 32 549
Fragments arranged by size:
835 549 32
HindIII A'AGCT_T
Cuts at: 0 1410 1416
Size: 1410 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1416
Size: 1316 100
MscI TGG'CCA
Cuts at: 0 116 1416
Size: 116 1300
MslI CAynn'nnrTG
Cuts at: 0 106 1271 1416
Size: 106 1165 145
Fragments arranged by size:
1165 145 106
NcoI C'CATG_G
Cuts at: 0 924 1416
Size: 924 492
NspI r_CATG'y
Cuts at: 0 678 781 1416
Size: 678 103 635
Fragments arranged by size:
678 635 103
PasI CC'CwG_GG
Cuts at: 0 797 1416
Size: 797 619
PciI A'CATG_T
Cuts at: 0 777 1416
Size: 777 639
PfoI T'CCnGG_A
Cuts at: 0 194 210 1416
Size: 194 16 1206
Fragments arranged by size:
1206 194 16
PmlI CAC'GTG
Cuts at: 0 1255 1416
Size: 1255 161
PpuMI rG'GwC_Cy
Cuts at: 0 727 1010 1416
Size: 727 283 406
Fragments arranged by size:
727 406 283
PsiI TTA'TAA
Cuts at: 0 905 1310 1416
Size: 905 405 106
Fragments arranged by size:
905 405 106
PstI C_TGCA'G
Cuts at: 0 23 1416
Size: 23 1393
SanDI GG'GwC_CC
Cuts at: 0 727 1416
Size: 727 689
SfcI C'TryA_G
Cuts at: 0 19 1383 1416
Size: 19 1364 33
Fragments arranged by size:
1364 33 19
SnaBI TAC'GTA
Cuts at: 0 299 1416
Size: 299 1117
StuI AGG'CCT
Cuts at: 0 1381 1416
Size: 1381 35
StyI C'CwwG_G
Cuts at: 0 415 896 924 976 1416
Size: 415 481 28 52 440
Fragments arranged by size:
481 440 415 52 28
SwaI ATTT'AAAT
Cuts at: 0 719 1416
Size: 719 697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 271 1416
Size: 271 1145
TatI w'GTAC_w
Cuts at: 0 1358 1416
Size: 1358 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 1416
Size: 122 32 1262
Fragments arranged by size:
1262 122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 705 1416
Size: 153 552 711
Fragments arranged by size:
711 552 153
XmnI GAAnn'nnTTC
Cuts at: 0 1094 1416
Size: 1094 322
Enzymes that do cut:
AccI AcuI AflIII ApaLI ApoI AvaI BaeI BanII
BbsI BcgI BclI Bme1580I BplI BpmI BsaI BsaAI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrGI BssSI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI
MscI MslI NcoI NspI PasI PciI PfoI PmlI
PpuMI PsiI PstI SanDI SfcI SnaBI StuI StyI
SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstZ17I
Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
SspI Tth111I XbaI XhoI XmaI ZraI