Restriction map for
pFN21AE5601
(Linear) (Six-base) MAPSORT of: KIEE5601.seq Check: 7193 from: 1 to: 1452
KIEE5601.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1124 1452
Size: 1124 328
AflIII A'CryG_T
Cuts at: 0 348 1452
Size: 348 1104
AlwNI CAG_nnn'CTG
Cuts at: 0 21 400 669 1167 1452
Size: 21 379 269 498 285
Fragments arranged by size:
498 379 285 269 21
ApoI r'AATT_y
Cuts at: 0 1038 1452
Size: 1038 414
AvaI C'yCGr_G
Cuts at: 0 42 71 1452
Size: 42 29 1381
Fragments arranged by size:
1381 42 29
BamHI G'GATC_C
Cuts at: 0 516 1452
Size: 516 936
BanI G'GyrC_C
Cuts at: 0 3 120 617 1452
Size: 3 117 497 835
Fragments arranged by size:
835 497 117 3
BanII G_rGCy'C
Cuts at: 0 341 410 433 968 1452
Size: 341 69 23 535 484
Fragments arranged by size:
535 484 341 69 23
BbeI G_GCGC'C
Cuts at: 0 7 124 621 1452
Size: 7 117 497 831
Fragments arranged by size:
831 497 117 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 351 1452
Size: 351 1101
BglI GCCn_nnn'nGGC
Cuts at: 0 215 1027 1180 1438 1452
Size: 215 812 153 258 14
Fragments arranged by size:
812 258 215 153 14
BlpI GC'TnA_GC
Cuts at: 0 432 1147 1452
Size: 432 715 305
Fragments arranged by size:
715 432 305
Bme1580I G_kGCm'C
Cuts at: 0 1363 1452
Size: 1363 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 370 1377 1452
Size: 370 1007 75
Fragments arranged by size:
1007 370 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 259 271 849 1072 1452
Size: 160 99 12 578 223 380
Fragments arranged by size:
578 380 223 160 99 12
BsaAI yAC'GTr
Cuts at: 0 351 1452
Size: 351 1101
BsaHI Gr'CG_yC
Cuts at: 0 4 121 618 1452
Size: 4 117 497 834
Fragments arranged by size:
834 497 117 4
BsaWI w'CCGG_w
Cuts at: 0 584 1452
Size: 584 868
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 397 427 904 934 1231 1261 1452
Size: 397 30 477 30 297 30 191
Fragments arranged by size:
477 397 297 191 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 736 739 846 1452
Size: 268 468 3 107 606
Fragments arranged by size:
606 468 268 107 3
BseYI C'CCAG_C
Cuts at: 0 30 65 183 400 884 1355 1452
Size: 30 35 118 217 484 471 97
Fragments arranged by size:
484 471 217 118 97 35 30
BsiEI CG_ry'CG
Cuts at: 0 159 201 1452
Size: 159 42 1251
Fragments arranged by size:
1251 159 42
BsiHKAI G_wGCw'C
Cuts at: 0 341 654 968 1452
Size: 341 313 314 484
Fragments arranged by size:
484 341 314 313
Bsp1286I G_dGCh'C
Cuts at: 0 341 410 433 654 968 1363 1452
Size: 341 69 23 221 314 395 89
Fragments arranged by size:
395 341 314 221 89 69 23
BsrBI CCG'CTC
Cuts at: 0 1322 1452
Size: 1322 130
BsrDI GCAATG_nn'
Cuts at: 0 912 1452
Size: 912 540
BsrFI r'CCGG_y
Cuts at: 0 284 1452
Size: 284 1168
BstEII G'GTnAC_C
Cuts at: 0 802 1452
Size: 802 650
BstXI CCAn_nnnn'nTGG
Cuts at: 0 798 821 1452
Size: 798 23 631
Fragments arranged by size:
798 631 23
BstYI r'GATC_y
Cuts at: 0 516 659 1244 1452
Size: 516 143 585 208
Fragments arranged by size:
585 516 208 143
BtgI C'CryG_G
Cuts at: 0 153 275 675 1139 1202 1239 1427 1452
Size: 153 122 400 464 63 37 188 25
Fragments arranged by size:
464 400 188 153 122 63 37 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1381 1452
Size: 1381 71
BtsI GCAGTG_nn'
Cuts at: 0 56 1452
Size: 56 1396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1184 1219 1452
Size: 1184 35 233
Fragments arranged by size:
1184 233 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 594 1452
Size: 594 858
EaeI y'GGCC_r
Cuts at: 0 53 156 198 1028 1181 1205 1430 1452
Size: 53 103 42 830 153 24 225 22
Fragments arranged by size:
830 225 153 103 53 42 24 22
EagI C'GGCC_G
Cuts at: 0 156 198 1452
Size: 156 42 1254
Fragments arranged by size:
1254 156 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 1452
Size: 196 1256
EcoICRI GAG'CTC
Cuts at: 0 339 966 1452
Size: 339 627 486
Fragments arranged by size:
627 486 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 259 271 849 1072 1452
Size: 160 99 12 578 223 380
Fragments arranged by size:
578 380 223 160 99 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 1452
Size: 526 926
EcoO109I rG'GnC_Cy
Cuts at: 0 666 938 1409 1452
Size: 666 272 471 43
Fragments arranged by size:
666 471 272 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 435 1452
Size: 435 1017
HaeII r_GCGC'y
Cuts at: 0 7 124 621 1452
Size: 7 117 497 831
Fragments arranged by size:
831 497 117 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 432 464 508 540 1181 1213
Size: 397 32 3 32 44 32 641 32
Cuts at: 1213 1452
Size: 239
Fragments arranged by size:
641 397 239 44 32 32 32 32
3
KasI G'GCGC_C
Cuts at: 0 3 120 617 1452
Size: 3 117 497 835
Fragments arranged by size:
835 497 117 3
MscI TGG'CCA
Cuts at: 0 1183 1207 1432 1452
Size: 1183 24 225 20
Fragments arranged by size:
1183 225 24 20
MslI CAynn'nnrTG
Cuts at: 0 819 1452
Size: 819 633
MspA1I CmG'CkG
Cuts at: 0 155 1355 1452
Size: 155 1200 97
Fragments arranged by size:
1200 155 97
NaeI GCC'GGC
Cuts at: 0 286 1452
Size: 286 1166
NarI GG'CG_CC
Cuts at: 0 4 121 618 1452
Size: 4 117 497 834
Fragments arranged by size:
834 497 117 4
NcoI C'CATG_G
Cuts at: 0 275 1427 1452
Size: 275 1152 25
Fragments arranged by size:
1152 275 25
NdeI CA'TA_TG
Cuts at: 0 591 1452
Size: 591 861
NgoMIV G'CCGG_C
Cuts at: 0 284 1452
Size: 284 1168
NotI GC'GGCC_GC
Cuts at: 0 198 1452
Size: 198 1254
NspI r_CATG'y
Cuts at: 0 873 1089 1227 1452
Size: 873 216 138 225
Fragments arranged by size:
873 225 216 138
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 400 669 1452
Size: 330 70 269 783
Fragments arranged by size:
783 330 269 70
PfoI T'CCnGG_A
Cuts at: 0 329 1452
Size: 329 1123
PmlI CAC'GTG
Cuts at: 0 351 1452
Size: 351 1101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 950 982 1452
Size: 712 32 206 32 470
Fragments arranged by size:
712 470 206 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 938 1452
Size: 938 514
PstI C_TGCA'G
Cuts at: 0 17 319 1273 1452
Size: 17 302 954 179
Fragments arranged by size:
954 302 179 17
PvuII CAG'CTG
Cuts at: 0 1355 1452
Size: 1355 97
SacI G_AGCT'C
Cuts at: 0 341 968 1452
Size: 341 627 484
Fragments arranged by size:
627 484 341
SacII CC_GC'GG
Cuts at: 0 156 1452
Size: 156 1296
SanDI GG'GwC_CC
Cuts at: 0 938 1452
Size: 938 514
SfcI C'TryA_G
Cuts at: 0 13 315 1068 1269 1452
Size: 13 302 753 201 183
Fragments arranged by size:
753 302 201 183 13
SfoI GGC'GCC
Cuts at: 0 5 122 619 1452
Size: 5 117 497 833
Fragments arranged by size:
833 497 117 5
SmaI CCC'GGG
Cuts at: 0 44 1452
Size: 44 1408
SphI G_CATG'C
Cuts at: 0 1227 1452
Size: 1227 225
StuI AGG'CCT
Cuts at: 0 91 994 1452
Size: 91 903 458
Fragments arranged by size:
903 458 91
StyI C'CwwG_G
Cuts at: 0 275 386 1118 1276 1427 1452
Size: 275 111 732 158 151 25
Fragments arranged by size:
732 275 158 151 111 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1452
Size: 790 662
TatI w'GTAC_w
Cuts at: 0 230 1452
Size: 230 1222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 354 367 375 386 407 644 676
Size: 335 19 13 8 11 21 237 32
Cuts at: 676 904 936 1075 1107 1231 1263 1452
Size: 228 32 139 32 124 32 189
Fragments arranged by size:
335 237 228 189 139 124 32 32
32 32 21 19 13 11 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 871 1038 1440 1452
Size: 871 167 402 12
Fragments arranged by size:
871 402 167 12
XmaI C'CCGG_G
Cuts at: 0 42 1452
Size: 42 1410
Enzymes that do cut:
AccI AflIII AlwNI ApoI AvaI BamHI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmrI BpmI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DrdI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI
PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI
SacII SanDI SfcI SfoI SmaI SphI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI DraI
DraIII EarI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NheI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI