Restriction map for
pFN21AE5593
(Linear) (Six-base) MAPSORT of: KIEE5593.seq Check: 6591 from: 1 to: 1260
KIEE5593.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AccI GT'mk_AC
Cuts at: 0 849 1260
Size: 849 411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1260
Size: 578 682
AfeI AGC'GCT
Cuts at: 0 65 1260
Size: 65 1195
AflII C'TTAA_G
Cuts at: 0 720 1260
Size: 720 540
AflIII A'CryG_T
Cuts at: 0 752 1260
Size: 752 508
ApaI G_GGCC'C
Cuts at: 0 25 1260
Size: 25 1235
ApaLI G'TGCA_C
Cuts at: 0 620 1260
Size: 620 640
AvaI C'yCGr_G
Cuts at: 0 17 486 1260
Size: 17 469 774
Fragments arranged by size:
774 469 17
BanI G'GyrC_C
Cuts at: 0 132 252 325 406 710 826 1260
Size: 132 120 73 81 304 116 434
Fragments arranged by size:
434 304 132 120 116 81 73
BanII G_rGCy'C
Cuts at: 0 25 635 1260
Size: 25 610 625
Fragments arranged by size:
625 610 25
BbeI G_GCGC'C
Cuts at: 0 136 329 410 1260
Size: 136 193 81 850
Fragments arranged by size:
850 193 136 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 1062 1260
Size: 86 976 198
Fragments arranged by size:
976 198 86
BbvCI CC'TCA_GC
Cuts at: 0 373 445 1260
Size: 373 72 815
Fragments arranged by size:
815 373 72
BfrBI ATG'CAT
Cuts at: 0 1155 1260
Size: 1155 105
BglI GCCn_nnn'nGGC
Cuts at: 0 610 1260
Size: 610 650
Bme1580I G_kGCm'C
Cuts at: 0 25 187 257 554 624 1260
Size: 25 162 70 297 70 636
Fragments arranged by size:
636 297 162 70 70 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 509 1260
Size: 509 751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1260
Size: 814 446
Bpu10I CC'TnA_GC
Cuts at: 0 373 445 1260
Size: 373 72 815
Fragments arranged by size:
815 373 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 910 1260
Size: 793 117 350
Fragments arranged by size:
793 350 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 90 1260
Size: 90 1170
BsaAI yAC'GTr
Cuts at: 0 276 753 1260
Size: 276 477 507
Fragments arranged by size:
507 477 276
BsaHI Gr'CG_yC
Cuts at: 0 42 133 326 407 1260
Size: 42 91 193 81 853
Fragments arranged by size:
853 193 91 81 42
BsaWI w'CCGG_w
Cuts at: 0 575 975 1260
Size: 575 400 285
Fragments arranged by size:
575 400 285
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 96 114 117 1260
Size: 78 18 18 3 1143
Fragments arranged by size:
1143 78 18 18 3
BseYI C'CCAG_C
Cuts at: 0 80 161 424 700 1260
Size: 80 81 263 276 560
Fragments arranged by size:
560 276 263 81 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1260
Size: 964 296
BsiEI CG_ry'CG
Cuts at: 0 343 1260
Size: 343 917
BsiHKAI G_wGCw'C
Cuts at: 0 16 205 317 624 1260
Size: 16 189 112 307 636
Fragments arranged by size:
636 307 189 112 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 510 1260
Size: 510 750
Bsp1286I G_dGCh'C
Cuts at: 0 16 25 187 205 257 317 554 624
Size: 16 9 162 18 52 60 237 70
Cuts at: 624 635 1260
Size: 11 625
Fragments arranged by size:
625 237 162 70 60 52 18 16
11 9
BspEI T'CCGG_A
Cuts at: 0 975 1260
Size: 975 285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 680 1260
Size: 272 408 580
Fragments arranged by size:
580 408 272
BsrBI CCG'CTC
Cuts at: 0 567 1260
Size: 567 693
BsrDI GCAATG_nn'
Cuts at: 0 501 1260
Size: 501 759
BsrFI r'CCGG_y
Cuts at: 0 295 322 338 956 1260
Size: 295 27 16 618 304
Fragments arranged by size:
618 304 295 27 16
BssHII G'CGCG_C
Cuts at: 0 214 399 1260
Size: 214 185 861
Fragments arranged by size:
861 214 185
BstEII G'GTnAC_C
Cuts at: 0 915 1260
Size: 915 345
Bsu36I CC'TnA_GG
Cuts at: 0 706 1260
Size: 706 554
BtgI C'CryG_G
Cuts at: 0 257 676 1260
Size: 257 419 584
Fragments arranged by size:
584 419 257
BtsI GCAGTG_nn'
Cuts at: 0 622 1260
Size: 622 638
DraIII CAC_nnn'GTG
Cuts at: 0 549 1260
Size: 549 711
DrdI GACnn_nn'nnGTC
Cuts at: 0 753 1260
Size: 753 507
EaeI y'GGCC_r
Cuts at: 0 308 340 359 461 650 1260
Size: 308 32 19 102 189 610
Fragments arranged by size:
610 308 189 102 32 19
EagI C'GGCC_G
Cuts at: 0 340 1260
Size: 340 920
EarI CTCTTCn'nnn_
Cuts at: 0 292 1260
Size: 292 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 138 147 367 625 1260
Size: 138 9 220 258 635
Fragments arranged by size:
635 258 220 138 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 814 1260
Size: 578 236 446
Fragments arranged by size:
578 446 236
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 1260
Size: 391 869
EcoO109I rG'GnC_Cy
Cuts at: 0 21 22 813 996 1260
Size: 21 1 791 183 264
Fragments arranged by size:
791 264 183 21 1
FspI TGC'GCA
Cuts at: 0 280 1260
Size: 280 980
HaeII r_GCGC'y
Cuts at: 0 67 136 329 410 1103 1260
Size: 67 69 193 81 693 157
Fragments arranged by size:
693 193 157 81 69 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1196 1228 1260
Size: 1196 32 32
Fragments arranged by size:
1196 32 32
HincII GTy'rAC
Cuts at: 0 234 1260
Size: 234 1026
HpaI GTT'AAC
Cuts at: 0 234 1260
Size: 234 1026
KasI G'GCGC_C
Cuts at: 0 132 325 406 1260
Size: 132 193 81 854
Fragments arranged by size:
854 193 132 81
MscI TGG'CCA
Cuts at: 0 652 1260
Size: 652 608
MslI CAynn'nnrTG
Cuts at: 0 595 1260
Size: 595 665
MspA1I CmG'CkG
Cuts at: 0 171 306 348 377 678 1260
Size: 171 135 42 29 301 582
Fragments arranged by size:
582 301 171 135 42 29
NaeI GCC'GGC
Cuts at: 0 324 340 1260
Size: 324 16 920
Fragments arranged by size:
920 324 16
NarI GG'CG_CC
Cuts at: 0 133 326 407 1260
Size: 133 193 81 853
Fragments arranged by size:
853 193 133 81
NgoMIV G'CCGG_C
Cuts at: 0 322 338 1260
Size: 322 16 922
Fragments arranged by size:
922 322 16
NsiI A_TGCA'T
Cuts at: 0 1157 1260
Size: 1157 103
PasI CC'CwG_GG
Cuts at: 0 884 1260
Size: 884 376
PfoI T'CCnGG_A
Cuts at: 0 869 1260
Size: 869 391
PpuMI rG'GwC_Cy
Cuts at: 0 813 996 1260
Size: 813 183 264
Fragments arranged by size:
813 264 183
PshAI GACnn'nnGTC
Cuts at: 0 41 470 1260
Size: 41 429 790
Fragments arranged by size:
790 429 41
PspOMI G'GGCC_C
Cuts at: 0 21 1260
Size: 21 1239
PstI C_TGCA'G
Cuts at: 0 629 1177 1260
Size: 629 548 83
Fragments arranged by size:
629 548 83
PvuII CAG'CTG
Cuts at: 0 306 1260
Size: 306 954
SacII CC_GC'GG
Cuts at: 0 679 1260
Size: 679 581
SbfI CC_TGCA'GG
Cuts at: 0 1177 1260
Size: 1177 83
SfcI C'TryA_G
Cuts at: 0 625 1173 1260
Size: 625 548 87
Fragments arranged by size:
625 548 87
SfoI GGC'GCC
Cuts at: 0 134 327 408 1260
Size: 134 193 81 852
Fragments arranged by size:
852 193 134 81
SmaI CCC'GGG
Cuts at: 0 19 1260
Size: 19 1241
SmlI C'TyrA_G
Cuts at: 0 720 808 925 1260
Size: 720 88 117 335
Fragments arranged by size:
720 335 117 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 1260
Size: 262 998
XbaI T'CTAG_A
Cuts at: 0 777 1260
Size: 777 483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 1260
Size: 522 738
XmaI C'CCGG_G
Cuts at: 0 17 1260
Size: 17 1243
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BstEII Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII HpaI KasI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI PasI
PfoI PpuMI PshAI PspOMI PstI PvuII SacII SbfI
SfcI SfoI SmaI SmlI TaqII XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AlfI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BglII BlpI BmgBI BmtI
BplI BsaBI BsaXI BsiWI BsmI BspHI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NspI PacI PciI PflMI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XhoI XmnI ZraI