(Linear) (Six-base) MAPSORT of: KIEE5585.seq  Check: 1376  from: 1  to: 1110



KIEE5585.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:06  ..

AccI GT'mk_AC

Cuts at:      0     257     407    1110 
   Size:        257     150     703

  Fragments arranged by size: 

                 703     257     150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     869    1110 
   Size:        106     763     241

  Fragments arranged by size: 

                 763     241     106
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     479     562    1110 
   Size:        479      83     548

  Fragments arranged by size: 

                 548     479      83
 
ApoI r'AATT_y

Cuts at:      0      92     635    1110 
   Size:         92     543     475

  Fragments arranged by size: 

                 543     475      92
 
BclI T'GATC_A

Cuts at:      0     755    1110 
   Size:        755     355
 
BfrBI ATG'CAT

Cuts at:      0     920    1110 
   Size:        920     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733    1110 
   Size:        733     377
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     564     594     924     954    1110 
   Size:        564      30     330      30     156

  Fragments arranged by size: 

                 564     330     156      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1068    1110 
   Size:       1068      42
 
BseYI C'CCAG_C

Cuts at:      0     481    1110 
   Size:        481     629
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     708    1110 
   Size:        708     402
 
BsmI GAATG_Cn'

Cuts at:      0     584     621    1110 
   Size:        584      37     489

  Fragments arranged by size: 

                 584     489      37
 
BspHI T'CATG_A

Cuts at:      0     563    1110 
   Size:        563     547
 
BsrDI GCAATG_nn'

Cuts at:      0     260     887    1110 
   Size:        260     627     223

  Fragments arranged by size: 

                 627     260     223
 
BstYI r'GATC_y

Cuts at:      0     880    1110 
   Size:        880     230
 
BtgI C'CryG_G

Cuts at:      0     509    1110 
   Size:        509     601
 
EaeI y'GGCC_r

Cuts at:      0     530    1110 
   Size:        530     580
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     561    1110 
   Size:        323     238     549

  Fragments arranged by size: 

                 549     323     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     733     869    1110 
   Size:        106     627     136     241

  Fragments arranged by size: 

                 627     241     136     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     612    1110 
   Size:        612     498
 
HaeII r_GCGC'y

Cuts at:      0     858    1110 
   Size:        858     252
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160    1110 
   Size:        128      32     950

  Fragments arranged by size: 

                 950     128      32
 
HindIII A'AGCT_T

Cuts at:      0     174    1110 
   Size:        174     936
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     114    1110 
   Size:        114     996
 
MscI TGG'CCA

Cuts at:      0     532    1110 
   Size:        532     578
 
MslI CAynn'nnrTG

Cuts at:      0      42     508     589    1110 
   Size:         42     466      81     521

  Fragments arranged by size: 

                 521     466      81      42
 
NcoI C'CATG_G

Cuts at:      0     509    1110 
   Size:        509     601
 
NsiI A_TGCA'T

Cuts at:      0     922    1110 
   Size:        922     188
 
NspI r_CATG'y

Cuts at:      0     165     285    1110 
   Size:        165     120     825

  Fragments arranged by size: 

                 825     165     120
 
PfoI T'CCnGG_A

Cuts at:      0     362    1110 
   Size:        362     748
 
SapI GCTCTTCn'nnn_

Cuts at:      0     323    1110 
   Size:        323     787
 
SspI AAT'ATT

Cuts at:      0     977    1101    1110 
   Size:        977     124       9

  Fragments arranged by size: 

                 977     124       9
 
StyI C'CwwG_G

Cuts at:      0     152     509    1110 
   Size:        152     357     601

  Fragments arranged by size: 

                 601     357     152
 
TatI w'GTAC_w

Cuts at:      0     335    1110 
   Size:        335     775
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     564     596    1110 
   Size:        564      32     514

  Fragments arranged by size: 

                 564     514      32
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI     BclI    BfrBI     BpmI    BsaXI
    BseRI    BseYI     BsgI     BsmI    BspHI    BsrDI    BstYI     BtgI
     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI     NcoI     NsiI     NspI     PfoI     SapI     SspI
     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI