Restriction map for
pFN21AE5584
(Linear) (Six-base) MAPSORT of: KIEE5584.seq Check: 5752 from: 1 to: 1095
KIEE5584.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1095
Size: 900 195
AflIII A'CryG_T
Cuts at: 0 883 1095
Size: 883 212
AhdI GACnn_n'nnGTC
Cuts at: 0 432 1095
Size: 432 663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1095
Size: 943 152
AlwNI CAG_nnn'CTG
Cuts at: 0 193 339 668 1095
Size: 193 146 329 427
Fragments arranged by size:
427 329 193 146
ApoI r'AATT_y
Cuts at: 0 196 965 1095
Size: 196 769 130
Fragments arranged by size:
769 196 130
BanII G_rGCy'C
Cuts at: 0 790 1095
Size: 790 305
BbvCI CC'TCA_GC
Cuts at: 0 256 1095
Size: 256 839
BclI T'GATC_A
Cuts at: 0 140 302 1095
Size: 140 162 793
Fragments arranged by size:
793 162 140
BlpI GC'TnA_GC
Cuts at: 0 352 1095
Size: 352 743
BmrI ACTGGGnnnn_n'
Cuts at: 0 135 181 1095
Size: 135 46 914
Fragments arranged by size:
914 135 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1095
Size: 986 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 337 445 956 1095
Size: 117 220 108 511 139
Fragments arranged by size:
511 220 139 117 108
Bpu10I CC'TnA_GC
Cuts at: 0 256 841 1095
Size: 256 585 254
Fragments arranged by size:
585 256 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1095
Size: 897 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 1095
Size: 135 960
BsaAI yAC'GTr
Cuts at: 0 884 1095
Size: 884 211
BsaBI GATnn'nnATC
Cuts at: 0 307 469 1095
Size: 307 162 626
Fragments arranged by size:
626 307 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 1095
Size: 115 30 950
Fragments arranged by size:
950 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 349 1095
Size: 193 156 746
Fragments arranged by size:
746 193 156
BseYI C'CCAG_C
Cuts at: 0 166 447 1017 1095
Size: 166 281 570 78
Fragments arranged by size:
570 281 166 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 486 692 985 1095
Size: 313 173 206 293 110
Fragments arranged by size:
313 293 206 173 110
BsiHKAI G_wGCw'C
Cuts at: 0 383 883 1095
Size: 383 500 212
Fragments arranged by size:
500 383 212
BsmI GAATG_Cn'
Cuts at: 0 624 1095
Size: 624 471
Bsp1286I G_dGCh'C
Cuts at: 0 383 790 883 1095
Size: 383 407 93 212
Fragments arranged by size:
407 383 212 93
BspHI T'CATG_A
Cuts at: 0 473 1095
Size: 473 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 1088 1095
Size: 356 732 7
Fragments arranged by size:
732 356 7
BsrDI GCAATG_nn'
Cuts at: 0 177 1095
Size: 177 918
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 408 1095
Size: 315 93 687
Fragments arranged by size:
687 315 93
Bsu36I CC'TnA_GG
Cuts at: 0 430 1095
Size: 430 665
BtgI C'CryG_G
Cuts at: 0 278 491 596 1095
Size: 278 213 105 499
Fragments arranged by size:
499 278 213 105
BtsI GCAGTG_nn'
Cuts at: 0 179 1095
Size: 179 916
DraI TTT'AAA
Cuts at: 0 17 1095
Size: 17 1078
DrdI GACnn_nn'nnGTC
Cuts at: 0 495 1095
Size: 495 600
EaeI y'GGCC_r
Cuts at: 0 275 410 536 866 953 1095
Size: 275 135 126 330 87 142
Fragments arranged by size:
330 275 142 135 126 87
EarI CTCTTCn'nnn_
Cuts at: 0 271 638 916 955 1081 1095
Size: 271 367 278 39 126 14
Fragments arranged by size:
367 278 271 126 39 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 337 445 900 956 1095
Size: 117 220 108 455 56 139
Fragments arranged by size:
455 220 139 117 108 56
EcoO109I rG'GnC_Cy
Cuts at: 0 42 405 1095
Size: 42 363 690
Fragments arranged by size:
690 363 42
EcoRI G'AATT_C
Cuts at: 0 196 965 1095
Size: 196 769 130
Fragments arranged by size:
769 196 130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 856 1095
Size: 856 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 1095
Size: 418 32 645
Fragments arranged by size:
645 418 32
MscI TGG'CCA
Cuts at: 0 277 412 538 868 955 1095
Size: 277 135 126 330 87 140
Fragments arranged by size:
330 277 140 135 126 87
MslI CAynn'nnrTG
Cuts at: 0 508 514 1095
Size: 508 6 581
Fragments arranged by size:
581 508 6
MspA1I CmG'CkG
Cuts at: 0 315 1095
Size: 315 780
NcoI C'CATG_G
Cuts at: 0 278 491 1095
Size: 278 213 604
Fragments arranged by size:
604 278 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 419 585 765 1095
Size: 419 166 180 330
Fragments arranged by size:
419 330 180 166
PmlI CAC'GTG
Cuts at: 0 884 1095
Size: 884 211
PpuMI rG'GwC_Cy
Cuts at: 0 405 1095
Size: 405 690
PstI C_TGCA'G
Cuts at: 0 703 1095
Size: 703 392
PvuII CAG'CTG
Cuts at: 0 315 1095
Size: 315 780
SfcI C'TryA_G
Cuts at: 0 699 1095
Size: 699 396
SmlI C'TyrA_G
Cuts at: 0 876 1095
Size: 876 219
StuI AGG'CCT
Cuts at: 0 322 874 1095
Size: 322 552 221
Fragments arranged by size:
552 322 221
StyI C'CwwG_G
Cuts at: 0 278 401 491 869 1052 1095
Size: 278 123 90 378 183 43
Fragments arranged by size:
378 278 183 123 90 43
TatI w'GTAC_w
Cuts at: 0 80 97 341 1095
Size: 80 17 244 754
Fragments arranged by size:
754 244 80 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 1095
Size: 115 32 948
Fragments arranged by size:
948 115 32
XbaI T'CTAG_A
Cuts at: 0 73 1095
Size: 73 1022
Enzymes that do cut:
AcuI AflIII AhdI AlfI AlwNI ApoI BanII BbvCI
BclI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BstXI Bsu36I BtgI BtsI
DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I MscI MslI MspA1I NcoI PflMI PmlI PpuMI
PstI PvuII SfcI SmlI StuI StyI TatI TstI
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmtI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraIII EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XcmI XhoI
XmaI XmnI ZraI