Restriction map for
pFN21AE5583
(Linear) (Six-base) MAPSORT of: KIEE5583.seq Check: 981 from: 1 to: 1086
KIEE5583.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AccI GT'mk_AC
Cuts at: 0 722 1086
Size: 722 364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 361 1086
Size: 33 328 725
Fragments arranged by size:
725 328 33
AleI CACnn'nnGTG
Cuts at: 0 382 991 1086
Size: 382 609 95
Fragments arranged by size:
609 382 95
AlwNI CAG_nnn'CTG
Cuts at: 0 228 396 976 1086
Size: 228 168 580 110
Fragments arranged by size:
580 228 168 110
ApoI r'AATT_y
Cuts at: 0 652 1086
Size: 652 434
BanI G'GyrC_C
Cuts at: 0 117 1086
Size: 117 969
BanII G_rGCy'C
Cuts at: 0 91 194 595 841 1086
Size: 91 103 401 246 245
Fragments arranged by size:
401 246 245 103 91
Bme1580I G_kGCm'C
Cuts at: 0 389 1086
Size: 389 697
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 994 1086
Size: 74 920 92
Fragments arranged by size:
920 92 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 205 1086
Size: 165 40 881
Fragments arranged by size:
881 165 40
Bpu10I CC'TnA_GC
Cuts at: 0 169 1086
Size: 169 917
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 363 1086
Size: 363 723
BseYI C'CCAG_C
Cuts at: 0 215 270 1086
Size: 215 55 816
Fragments arranged by size:
816 215 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 623 765 1086
Size: 301 322 142 321
Fragments arranged by size:
322 321 301 142
BsiHKAI G_wGCw'C
Cuts at: 0 143 194 666 841 1086
Size: 143 51 472 175 245
Fragments arranged by size:
472 245 175 143 51
Bsp1286I G_dGCh'C
Cuts at: 0 91 143 194 389 595 666 841 1086
Size: 91 52 51 195 206 71 175 245
Fragments arranged by size:
245 206 195 175 91 71 52 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 842 938 1086
Size: 842 96 148
Fragments arranged by size:
842 148 96
BsrGI T'GTAC_A
Cuts at: 0 254 1086
Size: 254 832
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 1086
Size: 270 816
BstYI r'GATC_y
Cuts at: 0 740 1086
Size: 740 346
Bsu36I CC'TnA_GG
Cuts at: 0 63 566 1086
Size: 63 503 520
Fragments arranged by size:
520 503 63
BtgI C'CryG_G
Cuts at: 0 377 503 1086
Size: 377 126 583
Fragments arranged by size:
583 377 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 480 1086
Size: 117 363 606
Fragments arranged by size:
606 363 117
BtsI GCAGTG_nn'
Cuts at: 0 887 1086
Size: 887 199
DraIII CAC_nnn'GTG
Cuts at: 0 736 1086
Size: 736 350
EaeI y'GGCC_r
Cuts at: 0 410 1086
Size: 410 676
EarI CTCTTCn'nnn_
Cuts at: 0 238 337 754 907 1086
Size: 238 99 417 153 179
Fragments arranged by size:
417 238 179 153 99
EcoICRI GAG'CTC
Cuts at: 0 192 839 1086
Size: 192 647 247
Fragments arranged by size:
647 247 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 165 205 361 1086
Size: 33 132 40 156 725
Fragments arranged by size:
725 156 132 40 33
HindIII A'AGCT_T
Cuts at: 0 1049 1086
Size: 1049 37
MscI TGG'CCA
Cuts at: 0 412 1086
Size: 412 674
MslI CAynn'nnrTG
Cuts at: 0 305 325 382 991 1086
Size: 305 20 57 609 95
Fragments arranged by size:
609 305 95 57 20
NcoI C'CATG_G
Cuts at: 0 503 1086
Size: 503 583
NspI r_CATG'y
Cuts at: 0 255 1086
Size: 255 831
PasI CC'CwG_GG
Cuts at: 0 846 1086
Size: 846 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 396 1086
Size: 180 216 690
Fragments arranged by size:
690 216 180
SacI G_AGCT'C
Cuts at: 0 194 841 1086
Size: 194 647 245
Fragments arranged by size:
647 245 194
SapI GCTCTTCn'nnn_
Cuts at: 0 238 337 754 1086
Size: 238 99 417 332
Fragments arranged by size:
417 332 238 99
SexAI A'CCwGG_T
Cuts at: 0 278 1086
Size: 278 808
SfcI C'TryA_G
Cuts at: 0 597 1086
Size: 597 489
StyI C'CwwG_G
Cuts at: 0 174 503 1086
Size: 174 329 583
Fragments arranged by size:
583 329 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 714 1086
Size: 339 375 372
Fragments arranged by size:
375 372 339
TatI w'GTAC_w
Cuts at: 0 254 329 1019 1086
Size: 254 75 690 67
Fragments arranged by size:
690 254 75 67
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI BanI BanII Bme1580I
BmrI BpmI Bpu10I BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI HindIII MscI MslI
NcoI NspI PasI PflMI SacI SapI SexAI SfcI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI