Restriction map for
pFN21AE5582
(Linear) (Six-base) MAPSORT of: KIEE5582.seq Check: 5066 from: 1 to: 1077
KIEE5582.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AccI GT'mk_AC
Cuts at: 0 536 1077
Size: 536 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 178 814 830 1077
Size: 158 20 636 16 247
Fragments arranged by size:
636 247 158 20 16
AflIII A'CryG_T
Cuts at: 0 835 1077
Size: 835 242
AleI CACnn'nnGTG
Cuts at: 0 955 1077
Size: 955 122
ApoI r'AATT_y
Cuts at: 0 240 1077
Size: 240 837
BanII G_rGCy'C
Cuts at: 0 730 846 1077
Size: 730 116 231
Fragments arranged by size:
730 231 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 705 819 1077
Size: 308 397 114 258
Fragments arranged by size:
397 308 258 114
BfrBI ATG'CAT
Cuts at: 0 93 1077
Size: 93 984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1077
Size: 875 202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1077
Size: 60 1017
BsaI GGTCTCn'nnnn_
Cuts at: 0 891 1077
Size: 891 186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 653 831 1077
Size: 653 178 246
Fragments arranged by size:
653 246 178
BseYI C'CCAG_C
Cuts at: 0 275 1077
Size: 275 802
BsiHKAI G_wGCw'C
Cuts at: 0 25 1077
Size: 25 1052
BsmI GAATG_Cn'
Cuts at: 0 454 1077
Size: 454 623
Bsp1286I G_dGCh'C
Cuts at: 0 25 730 846 1077
Size: 25 705 116 231
Fragments arranged by size:
705 231 116 25
BsrDI GCAATG_nn'
Cuts at: 0 473 1077
Size: 473 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 853 1077
Size: 853 224
EaeI y'GGCC_r
Cuts at: 0 785 1077
Size: 785 292
EarI CTCTTCn'nnn_
Cuts at: 0 229 889 1077
Size: 229 660 188
Fragments arranged by size:
660 229 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 178 814 830 875 1077
Size: 158 20 636 16 45 202
Fragments arranged by size:
636 202 158 45 20 16
EcoRV GAT'ATC
Cuts at: 0 657 1077
Size: 657 420
HincII GTy'rAC
Cuts at: 0 30 1077
Size: 30 1047
HpaI GTT'AAC
Cuts at: 0 30 1077
Size: 30 1047
MscI TGG'CCA
Cuts at: 0 787 1077
Size: 787 290
MslI CAynn'nnrTG
Cuts at: 0 232 892 934 955 1077
Size: 232 660 42 21 122
Fragments arranged by size:
660 232 122 42 21
NsiI A_TGCA'T
Cuts at: 0 95 1077
Size: 95 982
NspI r_CATG'y
Cuts at: 0 525 543 583 839 1077
Size: 525 18 40 256 238
Fragments arranged by size:
525 256 238 40 18
PciI A'CATG_T
Cuts at: 0 835 1077
Size: 835 242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 920 1077
Size: 888 32 157
Fragments arranged by size:
888 157 32
PstI C_TGCA'G
Cuts at: 0 188 1077
Size: 188 889
SfcI C'TryA_G
Cuts at: 0 184 984 1077
Size: 184 800 93
Fragments arranged by size:
800 184 93
SmlI C'TyrA_G
Cuts at: 0 39 1077
Size: 39 1038
SspI AAT'ATT
Cuts at: 0 944 1077
Size: 944 133
StyI C'CwwG_G
Cuts at: 0 399 1077
Size: 399 678
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 543 1077
Size: 543 534
TatI w'GTAC_w
Cuts at: 0 153 293 1077
Size: 153 140 784
Fragments arranged by size:
784 153 140
XbaI T'CTAG_A
Cuts at: 0 111 1077
Size: 111 966
XmnI GAAnn'nnTTC
Cuts at: 0 163 452 1077
Size: 163 289 625
Fragments arranged by size:
625 289 163
Enzymes that do cut:
AccI AcuI AflIII AleI ApoI BanII BbsI BfrBI
BpmI BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I
BsrDI BstXI EaeI EarI Eco57MI EcoRV HincII HpaI
MscI MslI NsiI NspI PciI PpiI PstI SfcI
SmlI SspI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HindIII KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XcmI XhoI XmaI ZraI