(Linear) (Six-base) MAPSORT of: KIEE5579.seq  Check: 7343  from: 1  to: 1065



KIEE5579.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:06  ..

AccI GT'mk_AC

Cuts at:      0    1013    1065 
   Size:       1013      52
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     809    1065 
   Size:        179     630     256

  Fragments arranged by size: 

                 630     256     179
 
AgeI A'CCGG_T

Cuts at:      0     214    1065 
   Size:        214     851
 
ApaI G_GGCC'C

Cuts at:      0     696    1065 
   Size:        696     369
 
ApoI r'AATT_y

Cuts at:      0     373     399     447    1041    1065 
   Size:        373      26      48     594      24

  Fragments arranged by size: 

                 594     373      48      26      24
 
AseI AT'TA_AT

Cuts at:      0     424    1065 
   Size:        424     641
 
BanII G_rGCy'C

Cuts at:      0     696    1065 
   Size:        696     369
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    1065 
   Size:        857     208
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     552    1065 
   Size:        552     513
 
BglII A'GATC_T

Cuts at:      0     328     792     903    1065 
   Size:        328     464     111     162

  Fragments arranged by size: 

                 464     328     162     111
 
BlpI GC'TnA_GC

Cuts at:      0      44    1065 
   Size:         44    1021
 
Bme1580I G_kGCm'C

Cuts at:      0     696    1065 
   Size:        696     369
 
BsaBI GATnn'nnATC

Cuts at:      0     734    1065 
   Size:        734     331
 
BsaWI w'CCGG_w

Cuts at:      0     214     775    1065 
   Size:        214     561     290

  Fragments arranged by size: 

                 561     290     214
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     167     691    1065 
   Size:        167     524     374

  Fragments arranged by size: 

                 524     374     167
 
BsiHKAI G_wGCw'C

Cuts at:      0      45    1065 
   Size:         45    1020
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     696    1065 
   Size:         45     651     369

  Fragments arranged by size: 

                 651     369      45
 
BspEI T'CCGG_A

Cuts at:      0     775    1065 
   Size:        775     290
 
BspHI T'CATG_A

Cuts at:      0      15    1065 
   Size:         15    1050
 
BsrDI GCAATG_nn'

Cuts at:      0     249    1065 
   Size:        249     816
 
BsrFI r'CCGG_y

Cuts at:      0     214    1065 
   Size:        214     851
 
BstYI r'GATC_y

Cuts at:      0     328     792     903    1065 
   Size:        328     464     111     162

  Fragments arranged by size: 

                 464     328     162     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     770    1065 
   Size:        770     295
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     161     545    1065 
   Size:        122      39     384     520

  Fragments arranged by size: 

                 520     384     122      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     809    1065 
   Size:        179     630     256

  Fragments arranged by size: 

                 630     256     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0     692     693    1065 
   Size:        692       1     372

  Fragments arranged by size: 

                 692     372       1
 
EcoRI G'AATT_C

Cuts at:      0     399    1065 
   Size:        399     666
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     190    1065 
   Size:        190     875
 
HincII GTy'rAC

Cuts at:      0    1014    1065 
   Size:       1014      51
 
MfeI C'AATT_G

Cuts at:      0     104     959    1065 
   Size:        104     855     106

  Fragments arranged by size: 

                 855     106     104
 
MspA1I CmG'CkG

Cuts at:      0    1009    1065 
   Size:       1009      56
 
NspI r_CATG'y

Cuts at:      0     440     765    1065 
   Size:        440     325     300

  Fragments arranged by size: 

                 440     325     300
 
PsiI TTA'TAA

Cuts at:      0     347    1065 
   Size:        347     718
 
PspOMI G'GGCC_C

Cuts at:      0     692    1065 
   Size:        692     373
 
PstI C_TGCA'G

Cuts at:      0     536    1065 
   Size:        536     529
 
SalI G'TCGA_C

Cuts at:      0    1012    1065 
   Size:       1012      53
 
ScaI AGT'ACT

Cuts at:      0     747    1065 
   Size:        747     318
 
SfcI C'TryA_G

Cuts at:      0     532    1065 
   Size:        532     533
 
SphI G_CATG'C

Cuts at:      0     765    1065 
   Size:        765     300
 
SspI AAT'ATT

Cuts at:      0      79    1065 
   Size:         79     986
 
TatI w'GTAC_w

Cuts at:      0     745     999    1065 
   Size:        745     254      66

  Fragments arranged by size: 

                 745     254      66
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     ApaI     ApoI     AseI    BanII     BbsI
    BciVI    BglII     BlpI Bme1580I    BsaBI    BsaWI    BseRI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BsrDI    BsrFI    BstYI    BtgZI     EarI
  Eco57MI EcoO109I    EcoRI     FalI   HincII     MfeI   MspA1I     NspI
     PsiI   PspOMI     PstI     SalI     ScaI     SfcI     SphI     SspI
     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI