Restriction map for
pFN21AE5574
(Linear) (Six-base) MAPSORT of: KIEE5574.seq Check: 6730 from: 1 to: 1362
KIEE5574.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AatII G_ACGT'C
Cuts at: 0 1194 1362
Size: 1194 168
AccI GT'mk_AC
Cuts at: 0 1262 1362
Size: 1262 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 669 1362
Size: 617 52 693
Fragments arranged by size:
693 617 52
ApaI G_GGCC'C
Cuts at: 0 212 1362
Size: 212 1150
ApaLI G'TGCA_C
Cuts at: 0 1237 1362
Size: 1237 125
ApoI r'AATT_y
Cuts at: 0 43 164 797 1362
Size: 43 121 633 565
Fragments arranged by size:
633 565 121 43
AvaI C'yCGr_G
Cuts at: 0 332 1362
Size: 332 1030
BamHI G'GATC_C
Cuts at: 0 1168 1362
Size: 1168 194
BanI G'GyrC_C
Cuts at: 0 912 1362
Size: 912 450
BanII G_rGCy'C
Cuts at: 0 212 809 881 967 1362
Size: 212 597 72 86 395
Fragments arranged by size:
597 395 212 86 72
BbeI G_GCGC'C
Cuts at: 0 916 1362
Size: 916 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 179 213 221 255 981 1015 1362
Size: 179 34 8 34 726 34 347
Fragments arranged by size:
726 347 179 34 34 34 8
BfrBI ATG'CAT
Cuts at: 0 1133 1362
Size: 1133 229
BglI GCCn_nnn'nGGC
Cuts at: 0 244 1362
Size: 244 1118
BlpI GC'TnA_GC
Cuts at: 0 599 1362
Size: 599 763
Bme1580I G_kGCm'C
Cuts at: 0 212 405 1241 1362
Size: 212 193 836 121
Fragments arranged by size:
836 212 193 121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 614 1362
Size: 614 748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 610 823 951 1362
Size: 42 568 213 128 411
Fragments arranged by size:
568 411 213 128 42
Bpu10I CC'TnA_GC
Cuts at: 0 589 671 961 1349 1362
Size: 589 82 290 388 13
Fragments arranged by size:
589 388 290 82 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 567 1362
Size: 567 795
BsaHI Gr'CG_yC
Cuts at: 0 913 1191 1362
Size: 913 278 171
Fragments arranged by size:
913 278 171
BsaWI w'CCGG_w
Cuts at: 0 1196 1362
Size: 1196 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 900 930 979 1009 1362
Size: 900 30 49 30 353
Fragments arranged by size:
900 353 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 871 1362
Size: 871 491
BseYI C'CCAG_C
Cuts at: 0 205 211 265 1362
Size: 205 6 54 1097
Fragments arranged by size:
1097 205 54 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 628 948 1131 1362
Size: 202 426 320 183 231
Fragments arranged by size:
426 320 231 202 183
BsiHKAI G_wGCw'C
Cuts at: 0 595 809 967 1241 1362
Size: 595 214 158 274 121
Fragments arranged by size:
595 274 214 158 121
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1198 1362
Size: 1198 164
Bsp1286I G_dGCh'C
Cuts at: 0 212 405 595 809 881 967 1241 1362
Size: 212 193 190 214 72 86 274 121
Fragments arranged by size:
274 214 212 193 190 121 86 72
BspHI T'CATG_A
Cuts at: 0 305 1362
Size: 305 1057
BsrBI CCG'CTC
Cuts at: 0 956 1362
Size: 956 406
BsrFI r'CCGG_y
Cuts at: 0 1084 1362
Size: 1084 278
BssSI C'ACGA_G
Cuts at: 0 604 1362
Size: 604 758
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 1362
Size: 265 1097
BstYI r'GATC_y
Cuts at: 0 1168 1362
Size: 1168 194
BtgI C'CryG_G
Cuts at: 0 482 1172 1362
Size: 482 690 190
Fragments arranged by size:
690 482 190
BtsI GCAGTG_nn'
Cuts at: 0 222 1239 1362
Size: 222 1017 123
Fragments arranged by size:
1017 222 123
EcoICRI GAG'CTC
Cuts at: 0 807 965 1362
Size: 807 158 397
Fragments arranged by size:
807 397 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 610 617 669 823 951 1362
Size: 42 568 7 52 154 128 411
Fragments arranged by size:
568 411 154 128 52 42 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 291 1362
Size: 291 1071
EcoO109I rG'GnC_Cy
Cuts at: 0 62 621 1362
Size: 62 559 741
Fragments arranged by size:
741 559 62
HaeII r_GCGC'y
Cuts at: 0 481 916 1362
Size: 481 435 446
Fragments arranged by size:
481 446 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 900 932 979 1011 1260 1292
Size: 85 32 783 32 47 32 249 32
Cuts at: 1292 1362
Size: 70
Fragments arranged by size:
783 249 85 70 47 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 912 1362
Size: 912 450
MfeI C'AATT_G
Cuts at: 0 535 1362
Size: 535 827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1362
Size: 668 694
MslI CAynn'nnrTG
Cuts at: 0 842 1362
Size: 842 520
MspA1I CmG'CkG
Cuts at: 0 186 265 1362
Size: 186 79 1097
Fragments arranged by size:
1097 186 79
NaeI GCC'GGC
Cuts at: 0 1086 1362
Size: 1086 276
NarI GG'CG_CC
Cuts at: 0 913 1362
Size: 913 449
NgoMIV G'CCGG_C
Cuts at: 0 1084 1362
Size: 1084 278
NsiI A_TGCA'T
Cuts at: 0 1135 1362
Size: 1135 227
PasI CC'CwG_GG
Cuts at: 0 150 1362
Size: 150 1212
PpuMI rG'GwC_Cy
Cuts at: 0 62 1362
Size: 62 1300
PshAI GACnn'nnGTC
Cuts at: 0 1190 1362
Size: 1190 172
PspOMI G'GGCC_C
Cuts at: 0 208 1362
Size: 208 1154
PstI C_TGCA'G
Cuts at: 0 477 511 1076 1362
Size: 477 34 565 286
Fragments arranged by size:
565 477 286 34
PvuII CAG'CTG
Cuts at: 0 186 265 1362
Size: 186 79 1097
Fragments arranged by size:
1097 186 79
SacI G_AGCT'C
Cuts at: 0 809 967 1362
Size: 809 158 395
Fragments arranged by size:
809 395 158
SfcI C'TryA_G
Cuts at: 0 288 473 507 781 1072 1362
Size: 288 185 34 274 291 290
Fragments arranged by size:
291 290 288 274 185 34
SfoI GGC'GCC
Cuts at: 0 914 1362
Size: 914 448
SmlI C'TyrA_G
Cuts at: 0 332 1362
Size: 332 1030
StyI C'CwwG_G
Cuts at: 0 143 983 1004 1362
Size: 143 840 21 358
Fragments arranged by size:
840 358 143 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 38 1362
Size: 38 1324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1135 1362
Size: 1135 227
XhoI C'TCGA_G
Cuts at: 0 332 1362
Size: 332 1030
ZraI GAC'GTC
Cuts at: 0 1192 1362
Size: 1192 170
Enzymes that do cut:
AatII AccI AcuI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI
BssSI BstXI BstYI BtgI BtsI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MfeI MmeI MslI MspA1I
NaeI NarI NgoMIV NsiI PasI PpuMI PshAI PspOMI
PstI PvuII SacI SfcI SfoI SmlI StyI TaqII
XcmI XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BclI BglII BmgBI BmrI
BmtI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BspEI
BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MluI MscI NcoI
NdeI NheI NotI NruI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XmaI
XmnI