Restriction map for
pFN21AE5570
(Linear) (Six-base) MAPSORT of: KIEE5570.seq Check: 7571 from: 1 to: 1365
KIEE5570.orf
With 182 enzymes: SgfI *
July 1, 2014 11:00 ..
AclI AA'CG_TT
Cuts at: 0 413 1365
Size: 413 952
AflIII A'CryG_T
Cuts at: 0 488 1365
Size: 488 877
AhdI GACnn_n'nnGTC
Cuts at: 0 402 1365
Size: 402 963
AlwNI CAG_nnn'CTG
Cuts at: 0 1180 1365
Size: 1180 185
ApoI r'AATT_y
Cuts at: 0 34 1224 1365
Size: 34 1190 141
Fragments arranged by size:
1190 141 34
BanI G'GyrC_C
Cuts at: 0 13 457 1365
Size: 13 444 908
Fragments arranged by size:
908 444 13
BfrBI ATG'CAT
Cuts at: 0 354 1188 1365
Size: 354 834 177
Fragments arranged by size:
834 354 177
BglII A'GATC_T
Cuts at: 0 443 1365
Size: 443 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 799 1365
Size: 506 293 566
Fragments arranged by size:
566 506 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 892 1365
Size: 385 507 473
Fragments arranged by size:
507 473 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 389 1365
Size: 389 976
BsaBI GATnn'nnATC
Cuts at: 0 311 1365
Size: 311 1054
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 835 865 1365
Size: 772 30 33 30 500
Fragments arranged by size:
772 500 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 313 1033 1060 1348 1351 1365
Size: 130 183 720 27 288 3 14
Fragments arranged by size:
720 288 183 130 27 14 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1365
Size: 1079 286
BsmI GAATG_Cn'
Cuts at: 0 356 1188 1365
Size: 356 832 177
Fragments arranged by size:
832 356 177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 1365
Size: 11 1354
BssSI C'ACGA_G
Cuts at: 0 798 1365
Size: 798 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 1365
Size: 205 1160
BstYI r'GATC_y
Cuts at: 0 443 1365
Size: 443 922
BtgI C'CryG_G
Cuts at: 0 511 1104 1365
Size: 511 593 261
Fragments arranged by size:
593 511 261
DraI TTT'AAA
Cuts at: 0 618 944 1365
Size: 618 326 421
Fragments arranged by size:
618 421 326
DrdI GACnn_nn'nnGTC
Cuts at: 0 972 1365
Size: 972 393
EaeI y'GGCC_r
Cuts at: 0 748 1365
Size: 748 617
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 856 1365
Size: 856 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 799 1365
Size: 506 293 566
Fragments arranged by size:
566 506 293
EcoO109I rG'GnC_Cy
Cuts at: 0 609 1365
Size: 609 756
EcoRI G'AATT_C
Cuts at: 0 1224 1365
Size: 1224 141
EcoRV GAT'ATC
Cuts at: 0 107 1155 1365
Size: 107 1048 210
Fragments arranged by size:
1048 210 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 388 420 772 804 1056 1088
Size: 75 32 281 32 352 32 252 32
Cuts at: 1088 1144 1176 1365
Size: 56 32 189
Fragments arranged by size:
352 281 252 189 75 56 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1266 1365
Size: 1266 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1001 1365
Size: 480 521 364
Fragments arranged by size:
521 480 364
NcoI C'CATG_G
Cuts at: 0 511 1104 1365
Size: 511 593 261
Fragments arranged by size:
593 511 261
NdeI CA'TA_TG
Cuts at: 0 365 1365
Size: 365 1000
NsiI A_TGCA'T
Cuts at: 0 356 1190 1365
Size: 356 834 175
Fragments arranged by size:
834 356 175
NspI r_CATG'y
Cuts at: 0 492 1212 1365
Size: 492 720 153
Fragments arranged by size:
720 492 153
PciI A'CATG_T
Cuts at: 0 488 1365
Size: 488 877
PfoI T'CCnGG_A
Cuts at: 0 776 1365
Size: 776 589
PpuMI rG'GwC_Cy
Cuts at: 0 609 1365
Size: 609 756
PstI C_TGCA'G
Cuts at: 0 1127 1365
Size: 1127 238
SfcI C'TryA_G
Cuts at: 0 1123 1365
Size: 1123 242
SmlI C'TyrA_G
Cuts at: 0 400 871 1365
Size: 400 471 494
Fragments arranged by size:
494 471 400
StyI C'CwwG_G
Cuts at: 0 494 511 1104 1365
Size: 494 17 593 261
Fragments arranged by size:
593 494 261 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 789 821 835 867 1365
Size: 789 32 14 32 498
Fragments arranged by size:
789 498 32 32 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 1365
Size: 1137 228
Enzymes that do cut:
AclI AflIII AhdI AlwNI ApoI BanI BfrBI BglII
BpmI BpuEI BsaI BsaBI BsaXI BseRI BsgI BsmI
BsmBI BssSI BstXI BstYI BtgI DraI DrdI EaeI
EciI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII MmeI
NcoI NdeI NsiI NspI PciI PfoI PpuMI PstI
SfcI SmlI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII EagI EarI EcoICRI
EcoNI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI