Restriction map for
pFN21AE5568
(Linear) (Six-base) MAPSORT of: KIEE5568.seq Check: 7300 from: 1 to: 1293
KIEE5568.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 480 1117 1293
Size: 480 637 176
Fragments arranged by size:
637 480 176
Acc65I G'GTAC_C
Cuts at: 0 103 1293
Size: 103 1190
AclI AA'CG_TT
Cuts at: 0 384 1293
Size: 384 909
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 1181 1293
Size: 711 470 112
Fragments arranged by size:
711 470 112
AflIII A'CryG_T
Cuts at: 0 511 1293
Size: 511 782
AleI CACnn'nnGTG
Cuts at: 0 1032 1293
Size: 1032 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 1293
Size: 691 602
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1095 1222 1293
Size: 1015 80 127 71
Fragments arranged by size:
1015 127 80 71
ApaI G_GGCC'C
Cuts at: 0 768 1293
Size: 768 525
AvaI C'yCGr_G
Cuts at: 0 462 818 1293
Size: 462 356 475
Fragments arranged by size:
475 462 356
AvrII C'CTAG_G
Cuts at: 0 63 1293
Size: 63 1230
BanI G'GyrC_C
Cuts at: 0 103 504 1129 1293
Size: 103 401 625 164
Fragments arranged by size:
625 401 164 103
BanII G_rGCy'C
Cuts at: 0 185 344 551 768 904 967 1027 1293
Size: 185 159 207 217 136 63 60 266
Fragments arranged by size:
266 217 207 185 159 136 63 60
BbeI G_GCGC'C
Cuts at: 0 508 1293
Size: 508 785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1055 1089 1293
Size: 1055 34 204
Fragments arranged by size:
1055 204 34
BfrBI ATG'CAT
Cuts at: 0 555 1293
Size: 555 738
BglI GCCn_nnn'nGGC
Cuts at: 0 851 1293
Size: 851 442
Bme1580I G_kGCm'C
Cuts at: 0 768 1134 1293
Size: 768 366 159
Fragments arranged by size:
768 366 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 764 968 1293
Size: 764 204 325
Fragments arranged by size:
764 325 204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 923 1293
Size: 923 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 309 382 502 1142 1293
Size: 66 243 73 120 640 151
Fragments arranged by size:
640 243 151 120 73 66
Bpu10I CC'TnA_GC
Cuts at: 0 1145 1293
Size: 1145 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 501 1293
Size: 22 479 792
Fragments arranged by size:
792 479 22
BsaAI yAC'GTr
Cuts at: 0 392 512 1293
Size: 392 120 781
Fragments arranged by size:
781 392 120
BsaHI Gr'CG_yC
Cuts at: 0 505 682 1293
Size: 505 177 611
Fragments arranged by size:
611 505 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 931 961 1293
Size: 931 30 332
Fragments arranged by size:
931 332 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 687 1293
Size: 687 606
BseYI C'CCAG_C
Cuts at: 0 33 343 561 1236 1293
Size: 33 310 218 675 57
Fragments arranged by size:
675 310 218 57 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1293
Size: 1085 208
BsiEI CG_ry'CG
Cuts at: 0 241 594 1293
Size: 241 353 699
Fragments arranged by size:
699 353 241
BsiHKAI G_wGCw'C
Cuts at: 0 551 581 1293
Size: 551 30 712
Fragments arranged by size:
712 551 30
Bsp1286I G_dGCh'C
Cuts at: 0 185 344 551 581 768 904 967 1027
Size: 185 159 207 30 187 136 63 60
Cuts at: 1027 1134 1293
Size: 107 159
Fragments arranged by size:
207 187 185 159 159 136 107 63
60 30
BspHI T'CATG_A
Cuts at: 0 543 1293
Size: 543 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 480 1117 1293
Size: 480 637 176
Fragments arranged by size:
637 480 176
BsrBI CCG'CTC
Cuts at: 0 907 1293
Size: 907 386
BsrFI r'CCGG_y
Cuts at: 0 731 1293
Size: 731 562
BssSI C'ACGA_G
Cuts at: 0 574 1293
Size: 574 719
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 1034 1293
Size: 892 142 259
Fragments arranged by size:
892 259 142
BstYI r'GATC_y
Cuts at: 0 626 1293
Size: 626 667
Bsu36I CC'TnA_GG
Cuts at: 0 131 1293
Size: 131 1162
BtgI C'CryG_G
Cuts at: 0 284 864 1293
Size: 284 580 429
Fragments arranged by size:
580 429 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 804 1293
Size: 804 489
BtsI GCAGTG_nn'
Cuts at: 0 483 1293
Size: 483 810
EaeI y'GGCC_r
Cuts at: 0 238 783 1163 1293
Size: 238 545 380 130
Fragments arranged by size:
545 380 238 130
EagI C'GGCC_G
Cuts at: 0 238 1293
Size: 238 1055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 967 1293
Size: 967 326
EcoICRI GAG'CTC
Cuts at: 0 549 1293
Size: 549 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 309 382 502 711 1142 1181 1293
Size: 66 243 73 120 209 431 39 112
Fragments arranged by size:
431 243 209 120 112 73 66 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1077 1124 1293
Size: 220 857 47 169
Fragments arranged by size:
857 220 169 47
EcoO109I rG'GnC_Cy
Cuts at: 0 309 765 822 860 875 1012 1293
Size: 309 456 57 38 15 137 281
Fragments arranged by size:
456 309 281 137 57 38 15
FspI TGC'GCA
Cuts at: 0 90 1293
Size: 90 1203
HaeII r_GCGC'y
Cuts at: 0 73 508 1293
Size: 73 435 785
Fragments arranged by size:
785 435 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 682 714 931 963 1293
Size: 682 32 217 32 330
Fragments arranged by size:
682 330 217 32 32
HincII GTy'rAC
Cuts at: 0 615 1293
Size: 615 678
KasI G'GCGC_C
Cuts at: 0 504 1293
Size: 504 789
KpnI G_GTAC'C
Cuts at: 0 107 1293
Size: 107 1186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 852 1293
Size: 276 576 441
Fragments arranged by size:
576 441 276
MslI CAynn'nnrTG
Cuts at: 0 445 556 1032 1293
Size: 445 111 476 261
Fragments arranged by size:
476 445 261 111
MspA1I CmG'CkG
Cuts at: 0 86 930 1293
Size: 86 844 363
Fragments arranged by size:
844 363 86
NaeI GCC'GGC
Cuts at: 0 733 1293
Size: 733 560
NarI GG'CG_CC
Cuts at: 0 505 1293
Size: 505 788
NcoI C'CATG_G
Cuts at: 0 284 1293
Size: 284 1009
NgoMIV G'CCGG_C
Cuts at: 0 731 1293
Size: 731 562
NsiI A_TGCA'T
Cuts at: 0 557 1293
Size: 557 736
NspI r_CATG'y
Cuts at: 0 559 678 1293
Size: 559 119 615
Fragments arranged by size:
615 559 119
PfoI T'CCnGG_A
Cuts at: 0 188 359 958 1293
Size: 188 171 599 335
Fragments arranged by size:
599 335 188 171
PmlI CAC'GTG
Cuts at: 0 392 512 1293
Size: 392 120 781
Fragments arranged by size:
781 392 120
PpuMI rG'GwC_Cy
Cuts at: 0 309 822 875 1293
Size: 309 513 53 418
Fragments arranged by size:
513 418 309 53
PshAI GACnn'nnGTC
Cuts at: 0 258 1293
Size: 258 1035
PspOMI G'GGCC_C
Cuts at: 0 764 1293
Size: 764 529
PstI C_TGCA'G
Cuts at: 0 844 929 1011 1293
Size: 844 85 82 282
Fragments arranged by size:
844 282 85 82
PvuII CAG'CTG
Cuts at: 0 86 1293
Size: 86 1207
SacI G_AGCT'C
Cuts at: 0 551 1293
Size: 551 742
SanDI GG'GwC_CC
Cuts at: 0 875 1293
Size: 875 418
SbfI CC_TGCA'GG
Cuts at: 0 1011 1293
Size: 1011 282
SfcI C'TryA_G
Cuts at: 0 840 925 1007 1293
Size: 840 85 82 286
Fragments arranged by size:
840 286 85 82
SfoI GGC'GCC
Cuts at: 0 506 1293
Size: 506 787
SmaI CCC'GGG
Cuts at: 0 820 1293
Size: 820 473
SmlI C'TyrA_G
Cuts at: 0 462 1293
Size: 462 831
StuI AGG'CCT
Cuts at: 0 145 1072 1119 1293
Size: 145 927 47 174
Fragments arranged by size:
927 174 145 47
StyI C'CwwG_G
Cuts at: 0 25 63 177 284 1293
Size: 25 38 114 107 1009
Fragments arranged by size:
1009 114 107 38 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 277 443 1022 1293
Size: 18 259 166 579 271
Fragments arranged by size:
579 271 259 166 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 786 818 1293
Size: 786 32 475
Fragments arranged by size:
786 475 32
XhoI C'TCGA_G
Cuts at: 0 462 1293
Size: 462 831
XmaI C'CCGG_G
Cuts at: 0 818 1293
Size: 818 475
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI AvaI AvrII BanI BanII BbeI BcgI BfrBI
BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SbfI SfcI SfoI SmaI SmlI StuI
StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI BaeI BamHI BbsI BbvCI
BciVI BclI BglII BlpI BmgBI BmtI BpuEI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EarI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI MfeI MluI MscI NdeI NheI NotI NruI
PacI PasI PciI PflMI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XmnI ZraI